BRENDA - Enzyme Database

Purification and characterization of the tyrosinase isozymes of pine needles

Kong, K.H.; Lee, J.L.; Park, H.J.; Cho, S.H.; Biochem. Mol. Biol. Int. 45, 717-724 (1998)

Data extracted from this reference:

Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
1.14.18.1
L-ascorbic acid
0.04 mM, complete inhibition
Pinus densiflora
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.14.18.1
8.7
-
L-Dopa
isoenzyme 1
Pinus densiflora
1.14.18.1
9.3
-
L-Dopa
isoenzyme 2
Pinus densiflora
1.14.18.1
10
-
L-Dopa
isoenzyme 3
Pinus densiflora
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
1.14.18.1
copper
1 mM, increase in activity
Pinus densiflora
1.14.18.1
Fe3+
1 mM, increase in activity
Pinus densiflora
1.14.18.1
Mg2+
1 mM, increase in activity
Pinus densiflora
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.14.18.1
45000
-
x * 45000, isoenzyme 3, SDS-PAGE
Pinus densiflora
1.14.18.1
55000
-
x * 55000, isoenzyme 2, SDS-PAGE
Pinus densiflora
1.14.18.1
65000
-
x * 65000, isoenzyme 1, SDS-PAGE
Pinus densiflora
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.14.18.1
Pinus densiflora
-
3 isoenzymes
-
Purification (Commentary)
EC Number
Commentary
Organism
1.14.18.1
Sephacryl S-200, Sephadex G-75, DEAE-Sephacel, isoenzymes 1-3
Pinus densiflora
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.14.18.1
(RS)-catechin + O2
isoenzymes 1-3, 96, 104, and 168% of activity with L-dopa respectively
636434
Pinus densiflora
?
-
-
-
?
1.14.18.1
3,4-dihydroxybenzoic acid + 1/2 O2
isoenzymes 1-3, 22%, 13%, and 13% of L-dopa activity respectively
636434
Pinus densiflora
(3,4-dioxocyclohexa-1,5-dien-1-yl)acetic acid + H2O
-
636434
Pinus densiflora
?
1.14.18.1
caffeic acid + 1/2 O2
-
636434
Pinus densiflora
caffeoyl quinone + H2O
-
636434
Pinus densiflora
?
1.14.18.1
catechol + 1/2 O2
-
636434
Pinus densiflora
1,2-benzoquinone + H2O
-
636434
Pinus densiflora
?
1.14.18.1
chlorogenic acid + O2
-
636434
Pinus densiflora
chlorogenoquinone + H2O
-
636434
Pinus densiflora
?
1.14.18.1
D-catechin + O2
isoenzymes 1, 2 and 3, 90%, 86% and 188% of L-dopa activity respectively
636434
Pinus densiflora
?
-
-
-
?
1.14.18.1
dopa + 1/2 O2
-
636434
Pinus densiflora
dopaquinone + H2O
-
636434
Pinus densiflora
?
1.14.18.1
L-epicatechin + O2
isoenzyme 1, 150% of L-dopa activity, isoenzyme 2 and 3, 170% and 175% of activity with L-dopa respectively
636434
Pinus densiflora
?
-
-
-
?
1.14.18.1
orcin + O2
isoenzymes 1-3, 41%, 33%, and 25% of activity with L-dopa respectively
636434
Pinus densiflora
?
-
-
-
?
1.14.18.1
p-coumaric acid + O2 + AH2
-
636434
Pinus densiflora
caffeic acid + H2O + A
-
636434
Pinus densiflora
?
1.14.18.1
pyrogallol + O2
isoenzymes 1-3, 210%, 263%, and 225% of activity with L-dopa respectively
636434
Pinus densiflora
?
-
-
-
?
1.14.18.1
resorcinol + O2
isoenzymes 1-3, 96%, 129%, and 100% of activity with L-dopa respectively
636434
Pinus densiflora
?
-
-
-
?
1.14.18.1
tyramine + O2
isoenzymes 1, 20% of L-dopa activity, isoenzyme2, 13% of activity with L-dopa, isoenzyme 3, 25% of activity with L-dopa
636434
Pinus densiflora
4-(2-aminoethyl)cyclohexa-3,5-diene-1,2-dione + H2O
-
636434
Pinus densiflora
?
Subunits
EC Number
Subunits
Commentary
Organism
1.14.18.1
?
x * 45000, isoenzyme 3, SDS-PAGE; x * 55000, isoenzyme 2, SDS-PAGE; x * 65000, isoenzyme 1, SDS-PAGE
Pinus densiflora
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
1.14.18.1
55
60
isoenzymes 1-3
Pinus densiflora
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.14.18.1
9
9.6
isoenzymes 1-3
Pinus densiflora
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
1.14.18.1
L-ascorbic acid
0.04 mM, complete inhibition
Pinus densiflora
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.14.18.1
8.7
-
L-Dopa
isoenzyme 1
Pinus densiflora
1.14.18.1
9.3
-
L-Dopa
isoenzyme 2
Pinus densiflora
1.14.18.1
10
-
L-Dopa
isoenzyme 3
Pinus densiflora
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
1.14.18.1
copper
1 mM, increase in activity
Pinus densiflora
1.14.18.1
Fe3+
1 mM, increase in activity
Pinus densiflora
1.14.18.1
Mg2+
1 mM, increase in activity
Pinus densiflora
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.14.18.1
45000
-
x * 45000, isoenzyme 3, SDS-PAGE
Pinus densiflora
1.14.18.1
55000
-
x * 55000, isoenzyme 2, SDS-PAGE
Pinus densiflora
1.14.18.1
65000
-
x * 65000, isoenzyme 1, SDS-PAGE
Pinus densiflora
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
1.14.18.1
Sephacryl S-200, Sephadex G-75, DEAE-Sephacel, isoenzymes 1-3
Pinus densiflora
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.14.18.1
(RS)-catechin + O2
isoenzymes 1-3, 96, 104, and 168% of activity with L-dopa respectively
636434
Pinus densiflora
?
-
-
-
?
1.14.18.1
3,4-dihydroxybenzoic acid + 1/2 O2
isoenzymes 1-3, 22%, 13%, and 13% of L-dopa activity respectively
636434
Pinus densiflora
(3,4-dioxocyclohexa-1,5-dien-1-yl)acetic acid + H2O
-
636434
Pinus densiflora
?
1.14.18.1
caffeic acid + 1/2 O2
-
636434
Pinus densiflora
caffeoyl quinone + H2O
-
636434
Pinus densiflora
?
1.14.18.1
catechol + 1/2 O2
-
636434
Pinus densiflora
1,2-benzoquinone + H2O
-
636434
Pinus densiflora
?
1.14.18.1
chlorogenic acid + O2
-
636434
Pinus densiflora
chlorogenoquinone + H2O
-
636434
Pinus densiflora
?
1.14.18.1
D-catechin + O2
isoenzymes 1, 2 and 3, 90%, 86% and 188% of L-dopa activity respectively
636434
Pinus densiflora
?
-
-
-
?
1.14.18.1
dopa + 1/2 O2
-
636434
Pinus densiflora
dopaquinone + H2O
-
636434
Pinus densiflora
?
1.14.18.1
L-epicatechin + O2
isoenzyme 1, 150% of L-dopa activity, isoenzyme 2 and 3, 170% and 175% of activity with L-dopa respectively
636434
Pinus densiflora
?
-
-
-
?
1.14.18.1
orcin + O2
isoenzymes 1-3, 41%, 33%, and 25% of activity with L-dopa respectively
636434
Pinus densiflora
?
-
-
-
?
1.14.18.1
p-coumaric acid + O2 + AH2
-
636434
Pinus densiflora
caffeic acid + H2O + A
-
636434
Pinus densiflora
?
1.14.18.1
pyrogallol + O2
isoenzymes 1-3, 210%, 263%, and 225% of activity with L-dopa respectively
636434
Pinus densiflora
?
-
-
-
?
1.14.18.1
resorcinol + O2
isoenzymes 1-3, 96%, 129%, and 100% of activity with L-dopa respectively
636434
Pinus densiflora
?
-
-
-
?
1.14.18.1
tyramine + O2
isoenzymes 1, 20% of L-dopa activity, isoenzyme2, 13% of activity with L-dopa, isoenzyme 3, 25% of activity with L-dopa
636434
Pinus densiflora
4-(2-aminoethyl)cyclohexa-3,5-diene-1,2-dione + H2O
-
636434
Pinus densiflora
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
1.14.18.1
?
x * 45000, isoenzyme 3, SDS-PAGE; x * 55000, isoenzyme 2, SDS-PAGE; x * 65000, isoenzyme 1, SDS-PAGE
Pinus densiflora
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
1.14.18.1
55
60
isoenzymes 1-3
Pinus densiflora
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.14.18.1
9
9.6
isoenzymes 1-3
Pinus densiflora