Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Pomerantz, S.H.; Murthy, V.V.
    Purification and properties of tyrosinases from Vibrio tyrosinaticus (1974), Arch. Biochem. Biophys., 160, 73-82.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.14.18.1 CN-
-
 Vibrio tyrosinaticus
1.14.18.1 diethyldithiocarbamate 10 mM, 80% inactivation, activity is restored by incubation with 0.01 mM Cu2+, Mn2+, Cd2+ or Fe2+ Vibrio tyrosinaticus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.14.18.1 2.2
-
 D-tyrosine isoenzyme A Vibrio tyrosinaticus
1.14.18.1 3.1
-
 L-tyrosine isoenzyme A and B Vibrio tyrosinaticus
1.14.18.1 36
-
 L-Dopa isoenzyme A Vibrio tyrosinaticus
1.14.18.1 67
-
 L-Dopa isoenzyme B Vibrio tyrosinaticus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.14.18.1 38500
-
 isoenzyme B, gel filtration Vibrio tyrosinaticus
1.14.18.1 40000
-
 isoenzyme A, gel filtration Vibrio tyrosinaticus

Organism

EC Number Organism UniProt Comment Textmining
1.14.18.1 Vibrio tyrosinaticus
-
 2 isoenzymes
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.14.18.1
-
 Vibrio tyrosinaticus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.14.18.1 46
-
 isoenzyme A Vibrio tyrosinaticus
1.14.18.1 137
-
 isoenzyme B Vibrio tyrosinaticus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.18.1 2 L-dopa + O2
-
 Vibrio tyrosinaticus 2 dopaquinone + 2 H2O
-
 r
1.14.18.1 D-tyrosine + O2
-
 Vibrio tyrosinaticus ?
-
 r
1.14.18.1 L-dopa + 1/2 O2
-
 Vibrio tyrosinaticus dopaquinone + H2O
-
 r
1.14.18.1 L-DOPA + O2
-
 Vibrio tyrosinaticus dopaquinone + H2O
-
 r
1.14.18.1 L-tyrosine + O2
-
 Vibrio tyrosinaticus dopaquinone + H2O
-
 r
1.14.18.1 L-tyrosine + O2
-
 Vibrio tyrosinaticus L-DOPA + H2O
-
 r

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.14.18.1 75
-
 L-Dopa isoenzyme A Vibrio tyrosinaticus
1.14.18.1 90
-
 L-Dopa isoenzyme B Vibrio tyrosinaticus
1.14.18.1 100
-
 L-tyrosine isoenzyme A and B Vibrio tyrosinaticus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.14.18.1 6.6 7.8
-
 Vibrio tyrosinaticus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.14.18.1 5.7 7.8 pH 5.7: 50% of maximal activity, pH 6.6-7.8: optimum Vibrio tyrosinaticus

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.14.18.1 0.095
-
 CN- competitive vs. tyrosine Vibrio tyrosinaticus
1.14.18.1 0.267
-
 diethyldithiocarbamate noncompetitive vs. tyrosine Vibrio tyrosinaticus