BRENDA - Enzyme Database

Catechol oxidase from green olives: properties and partial purification

Ben-Shalom, N.; Kahn, V.; Harel, E.; Mayer, A.M.; Phytochemistry 16, 1153-1158 (1977)
No PubMed abstract available

Data extracted from this reference:

Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
1.14.18.1
2-mercaptoethanol
0.029 mM, 50% inhibition
Olea europaea
1.14.18.1
8-hydroxyquinoline
1.3 mM, 50% inhibition
Olea europaea
1.14.18.1
Benzoic acid
0.2 mM, 50% inhibition
Olea europaea
1.14.18.1
citrate
-
Olea europaea
1.14.18.1
Cl-
6 mM, 50% inhibition at pH 4.5, 34 mM, 50% inhibition at pH 5
Olea europaea
1.14.18.1
diethyldithiocarbamate
0.0051 mM, 50% inhibition
Olea europaea
1.14.18.1
Salicylaldoxime
0.43 mM, 50% inhibition
Olea europaea
1.14.18.1
Sodium bisulfite
0.062 mM, 50% inhibition
Olea europaea
1.14.18.1
Thiourea
0.8 mM, 50% inhibition
Olea europaea
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.14.18.1
3.3
-
4-methylcatechol
-
Olea europaea
1.14.18.1
5
-
chlorogenic acid
-
Olea europaea
1.14.18.1
8.3
-
catechol
-
Olea europaea
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.14.18.1
42000
-
gel filtration
Olea europaea
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.14.18.1
Olea europaea
-
green olive
-
Purification (Commentary)
EC Number
Commentary
Organism
1.14.18.1
ammonium sulfate, Sephadex G-100, DEAE cellulose, partially purified
Olea europaea
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
1.14.18.1
fruit
-
Olea europaea
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.14.18.1
additional information
-
0.281 units, 1 unit is defined as 0.0024 ml O2/min
Olea europaea
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.14.18.1
4-methylcatechol + O2
-
636403
Olea europaea
4-methyl-o-benzoquinone + H2O
-
636403
Olea europaea
?
1.14.18.1
catechol + 1/2 O2
-
636403
Olea europaea
1,2-benzoquinone + H2O
-
636403
Olea europaea
?
1.14.18.1
chlorogenic acid + O2
-
636403
Olea europaea
chlorogenoquinone + H2O
-
636403
Olea europaea
?
1.14.18.1
D-catechin + O2
-
636403
Olea europaea
?
-
-
-
?
1.14.18.1
dopamine + 1/2 O2
-
636403
Olea europaea
dopamine quinone + H2O
-
636403
Olea europaea
?
1.14.18.1
protocatechuic acid + O2
-
636403
Olea europaea
?
-
-
-
?
Temperature Stability [°C]
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
1.14.18.1
70
-
half-life 15 min
Olea europaea
1.14.18.1
75
-
half-life 19 min
Olea europaea
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.14.18.1
4.5
-
-
Olea europaea
pH Range
EC Number
pH Minimum
pH Maximum
Commentary
Organism
1.14.18.1
3.5
6
about 40% of maximal activity at pH 3.4 and pH 6.0, catechol oxidase in Na-acetate buffer
Olea europaea
Ki Value [mM]
EC Number
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
1.14.18.1
0.2
-
Benzoic acid
-
Olea europaea
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
1.14.18.1
2-mercaptoethanol
0.029 mM, 50% inhibition
Olea europaea
1.14.18.1
8-hydroxyquinoline
1.3 mM, 50% inhibition
Olea europaea
1.14.18.1
Benzoic acid
0.2 mM, 50% inhibition
Olea europaea
1.14.18.1
citrate
-
Olea europaea
1.14.18.1
Cl-
6 mM, 50% inhibition at pH 4.5, 34 mM, 50% inhibition at pH 5
Olea europaea
1.14.18.1
diethyldithiocarbamate
0.0051 mM, 50% inhibition
Olea europaea
1.14.18.1
Salicylaldoxime
0.43 mM, 50% inhibition
Olea europaea
1.14.18.1
Sodium bisulfite
0.062 mM, 50% inhibition
Olea europaea
1.14.18.1
Thiourea
0.8 mM, 50% inhibition
Olea europaea
Ki Value [mM] (protein specific)
EC Number
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
1.14.18.1
0.2
-
Benzoic acid
-
Olea europaea
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.14.18.1
3.3
-
4-methylcatechol
-
Olea europaea
1.14.18.1
5
-
chlorogenic acid
-
Olea europaea
1.14.18.1
8.3
-
catechol
-
Olea europaea
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.14.18.1
42000
-
gel filtration
Olea europaea
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
1.14.18.1
ammonium sulfate, Sephadex G-100, DEAE cellulose, partially purified
Olea europaea
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
1.14.18.1
fruit
-
Olea europaea
-
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.14.18.1
additional information
-
0.281 units, 1 unit is defined as 0.0024 ml O2/min
Olea europaea
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.14.18.1
4-methylcatechol + O2
-
636403
Olea europaea
4-methyl-o-benzoquinone + H2O
-
636403
Olea europaea
?
1.14.18.1
catechol + 1/2 O2
-
636403
Olea europaea
1,2-benzoquinone + H2O
-
636403
Olea europaea
?
1.14.18.1
chlorogenic acid + O2
-
636403
Olea europaea
chlorogenoquinone + H2O
-
636403
Olea europaea
?
1.14.18.1
D-catechin + O2
-
636403
Olea europaea
?
-
-
-
?
1.14.18.1
dopamine + 1/2 O2
-
636403
Olea europaea
dopamine quinone + H2O
-
636403
Olea europaea
?
1.14.18.1
protocatechuic acid + O2
-
636403
Olea europaea
?
-
-
-
?
Temperature Stability [°C] (protein specific)
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
1.14.18.1
70
-
half-life 15 min
Olea europaea
1.14.18.1
75
-
half-life 19 min
Olea europaea
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.14.18.1
4.5
-
-
Olea europaea
pH Range (protein specific)
EC Number
pH Minimum
pH Maximum
Commentary
Organism
1.14.18.1
3.5
6
about 40% of maximal activity at pH 3.4 and pH 6.0, catechol oxidase in Na-acetate buffer
Olea europaea