BRENDA - Enzyme Database

Mono- and diphenolase activity from fruit of Malus pumila

Goodenough, P.W.; Kessell, S.; Lea, A.G.H.; Loeffler, T.; Phytochemistry 22, 359-363 (1983)
No PubMed abstract available

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
1.14.18.1
L-Dopa
and analogs, activation
Malus sp.
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
1.14.18.1
O2
at concentrations above 30%
Malus sp.
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.14.18.1
additional information
-
additional information
4.3% O2
Malus sp.
1.14.18.1
0.2
-
3-hydroxyphloridzin
-
Malus sp.
1.14.18.1
0.2
-
caffeic acid
-
Malus sp.
1.14.18.1
2.7
-
L-Dopa
-
Malus sp.
1.14.18.1
3.2
-
4-methylcatechol
-
Malus sp.
1.14.18.1
4
-
p-coumaric acid
-
Malus sp.
1.14.18.1
4.7
-
catechin
-
Malus sp.
1.14.18.1
5.7
-
epicatechin
-
Malus sp.
1.14.18.1
5.9
-
chlorogenic acid
-
Malus sp.
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.14.18.1
26000
-
x * 26000, SDS-PAGE
Malus sp.
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.14.18.1
Malus sp.
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
1.14.18.1
-
Malus sp.
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
1.14.18.1
fruit
-
Malus sp.
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.14.18.1
3-hydroxyphloridzin + O2
-
636382
Malus sp.
?
-
-
-
?
1.14.18.1
4-methylcatechol + O2
-
636382
Malus sp.
4-methyl-o-benzoquinone + H2O
-
-
-
-
1.14.18.1
caffeic acid + 1/2 O2
-
636382
Malus sp.
caffeoyl quinone + H2O
-
636382
Malus sp.
?
1.14.18.1
chlorogenic acid + O2
-
636382
Malus sp.
chlorogenoquinone + H2O
-
-
-
-
1.14.18.1
D-catechin + O2
-
636382
Malus sp.
?
-
-
-
?
1.14.18.1
epicatechin + O2
-
636382
Malus sp.
?
-
-
-
-
1.14.18.1
L-epicatechin + O2
-
636382
Malus sp.
?
-
-
-
?
1.14.18.1
p-coumaric acid + O2 + AH2
-
636382
Malus sp.
caffeic acid + H2O + A
-
636382
Malus sp.
?
1.14.18.1
phloridzin + O2
-
636382
Malus sp.
?
-
-
-
?
Subunits
EC Number
Subunits
Commentary
Organism
1.14.18.1
?
x * 26000, SDS-PAGE
Malus sp.
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.14.18.1
4.5
-
minimal activity at pH 5.5
Malus sp.
pH Range
EC Number
pH Minimum
pH Maximum
Commentary
Organism
1.14.18.1
5.5
-
no activity below
Malus sp.
pH Stability
EC Number
pH Stability
pH Stability Maximum
Commentary
Organism
1.14.18.1
3.5
4.5
optimal stability at pH 4.5, less stable above pH 6.0
Malus sp.
1.14.18.1
6
-
4 days at 2C, 70% inactivation
Malus sp.
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
1.14.18.1
L-Dopa
and analogs, activation
Malus sp.
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
1.14.18.1
O2
at concentrations above 30%
Malus sp.
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.14.18.1
additional information
-
additional information
4.3% O2
Malus sp.
1.14.18.1
0.2
-
3-hydroxyphloridzin
-
Malus sp.
1.14.18.1
0.2
-
caffeic acid
-
Malus sp.
1.14.18.1
2.7
-
L-Dopa
-
Malus sp.
1.14.18.1
3.2
-
4-methylcatechol
-
Malus sp.
1.14.18.1
4
-
p-coumaric acid
-
Malus sp.
1.14.18.1
4.7
-
catechin
-
Malus sp.
1.14.18.1
5.7
-
epicatechin
-
Malus sp.
1.14.18.1
5.9
-
chlorogenic acid
-
Malus sp.
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.14.18.1
26000
-
x * 26000, SDS-PAGE
Malus sp.
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
1.14.18.1
-
Malus sp.
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
1.14.18.1
fruit
-
Malus sp.
-
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.14.18.1
3-hydroxyphloridzin + O2
-
636382
Malus sp.
?
-
-
-
?
1.14.18.1
4-methylcatechol + O2
-
636382
Malus sp.
4-methyl-o-benzoquinone + H2O
-
-
-
-
1.14.18.1
caffeic acid + 1/2 O2
-
636382
Malus sp.
caffeoyl quinone + H2O
-
636382
Malus sp.
?
1.14.18.1
chlorogenic acid + O2
-
636382
Malus sp.
chlorogenoquinone + H2O
-
-
-
-
1.14.18.1
D-catechin + O2
-
636382
Malus sp.
?
-
-
-
?
1.14.18.1
epicatechin + O2
-
636382
Malus sp.
?
-
-
-
-
1.14.18.1
L-epicatechin + O2
-
636382
Malus sp.
?
-
-
-
?
1.14.18.1
p-coumaric acid + O2 + AH2
-
636382
Malus sp.
caffeic acid + H2O + A
-
636382
Malus sp.
?
1.14.18.1
phloridzin + O2
-
636382
Malus sp.
?
-
-
-
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
1.14.18.1
?
x * 26000, SDS-PAGE
Malus sp.
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.14.18.1
4.5
-
minimal activity at pH 5.5
Malus sp.
pH Range (protein specific)
EC Number
pH Minimum
pH Maximum
Commentary
Organism
1.14.18.1
5.5
-
no activity below
Malus sp.
pH Stability (protein specific)
EC Number
pH Stability
pH Stability Maximum
Commentary
Organism
1.14.18.1
3.5
4.5
optimal stability at pH 4.5, less stable above pH 6.0
Malus sp.
1.14.18.1
6
-
4 days at 2C, 70% inactivation
Malus sp.