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Literature summary extracted from

  • Wittenberg, C.; Triplett, E.L.
    A detergent-activated tyrosinase from Xenopus laevis. I. Purification and partial characterization (1985), J. Biol. Chem., 260, 12535-12541.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.14.18.1 SDS 3.0 mM, stimulates most effective of the anionic detergents tested Xenopus laevis
1.14.18.1 sodium deoxycholate approx. 60% activation Xenopus laevis
1.14.18.1 sodium dodecyl deoxycholate 3.0 mM, stimulates Xenopus laevis
1.14.18.1 sodium dodecyl sarcosinate 3.0 mM, stimulates Xenopus laevis
1.14.18.1 sodium dodecylsarcosinate approx. 70% activation Xenopus laevis
1.14.18.1 sodium dodecylsulfate 1 mM, 90% activation Xenopus laevis

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.14.18.1 5-hydroxyindole
-
 Xenopus laevis
1.14.18.1 5-hydroxytryptophan
-
 Xenopus laevis
1.14.18.1 CN- noncompetitive vs. dopa and tyrosine Xenopus laevis
1.14.18.1 diethyldithiocarbamate noncompetitive vs. dopa and tyrosine Xenopus laevis
1.14.18.1 additional information not inhibited by 2,2'-dipyridyl Xenopus laevis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.14.18.1 0.4
-
 L-tyrosine
-
 Xenopus laevis
1.14.18.1 0.6
-
 Dopa
-
 Xenopus laevis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.14.18.1 175000
-
 1 * 175000, SDS-PAGE Xenopus laevis
1.14.18.1 175000 230000 SDS-PAGE in the presence of urea Xenopus laevis
1.14.18.1 220000 229000 gel filtration in the absence and presence of SDS Xenopus laevis

Organism

EC Number Organism UniProt Comment Textmining
1.14.18.1 Xenopus laevis
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
1.14.18.1 glycoprotein
-
 Xenopus laevis

Purification (Commentary)

EC Number Purification (Comment) Organism
1.14.18.1
-
 Xenopus laevis

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.14.18.1 skin
-
 Xenopus laevis
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.14.18.1 1.43
-
 tyrosine hydroxylase activity Xenopus laevis
1.14.18.1 10.4
-
 dopa oxidation Xenopus laevis

Storage Stability

EC Number Storage Stability Organism
1.14.18.1 liquid N2 or -70°C, phosphate buffer pH 7.2, 150 mM NaCl, several months, no loss of activity Xenopus laevis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.18.1 Dopa + O2
-
 Xenopus laevis dopaquinone + H2O
-
 r
1.14.18.1 L-tyrosine + O2
-
 Xenopus laevis dopaquinone + H2O
-
 r
1.14.18.1 L-tyrosine + O2
-
 Xenopus laevis L-DOPA + H2O
-
 r

Subunits

EC Number Subunits Comment Organism
1.14.18.1 monomer 1 * 175000, SDS-PAGE Xenopus laevis

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.14.18.1 3.33
-
 L-tyrosine tyrosine hydroxylase activity Xenopus laevis
1.14.18.1 26.7
-
 Dopa dopa oxidase activity Xenopus laevis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.14.18.1 5.5 7.3 dopa oxidase activity Xenopus laevis
1.14.18.1 6.8 7.8 tyrosine hydroxylase activity Xenopus laevis

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.14.18.1 0.005
-
 5-hydroxyindole dopa oxidase inhibition Xenopus laevis
1.14.18.1 0.03
-
 diethyldithiocarbamate dopa oxidase inhibition Xenopus laevis
1.14.18.1 0.03
-
 diethyldithiocarbamate tyrosine hydroxylase Xenopus laevis
1.14.18.1 0.08
-
 CN- tyrosine hydroxylase inhibition Xenopus laevis
1.14.18.1 0.09
-
 CN- dopa oxidase inhibition Xenopus laevis