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Literature summary extracted from
- Partial purification and some properties of polyphenoloxidases from sago palm (1988), Agric. Biol. Chem., 52, 2215-2222.
No PubMed abstract available
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.14.18.1 | CN- | 0.2 mM, 50% inhibition of isoenzyme I, 0.13 mM, 50% inhibition of isoenzyme III | Metroxylon sagu |  |
| 1.14.18.1 | diethyldithiocarbamate | 0.5 mM, 50% inhibition of isoenzyme I, 0.19 mM, 50% inhibition of isoenzyme III | Metroxylon sagu | |
| 1.14.18.1 | EDTA | no inhibition EDTA | Metroxylon sagu | |
| 1.14.18.1 | Sodium sulfate | - |
Metroxylon sagu | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.14.18.1 | 3 | - |
D-catechin | isoenzyme III | Metroxylon sagu | |
| 1.14.18.1 | 3.03 | - |
DL-epicatechin | isoenzyme I | Metroxylon sagu | |
| 1.14.18.1 | 3.22 | - |
D-epicatechin | isoenzyme III | Metroxylon sagu | |
| 1.14.18.1 | 3.85 | - |
L-epicatechin | isoenzyme I | Metroxylon sagu | |
| 1.14.18.1 | 5 | - |
D-catechin | isoenzyme I | Metroxylon sagu | |
| 1.14.18.1 | 5.26 | - |
DL-epicatechin | isoenzyme III | Metroxylon sagu | |
| 1.14.18.1 | 6.67 | - |
D-epicatechin | isoenzyme I | Metroxylon sagu | |
| 1.14.18.1 | 11.1 | - |
L-epicatechin | isoenzyme III | Metroxylon sagu | |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.14.18.1 | Co2+ | 0.9 mM, slight activation of isoenzymes I and III | Metroxylon sagu | |
| 1.14.18.1 | copper | 0.1 mM, approx. 3fold activation of isoenzymes I and III | Metroxylon sagu | |
| 1.14.18.1 | Mn2+ | slight activation of isoenzymes I and III | Metroxylon sagu | |
| 1.14.18.1 | Ni2+ | 0.9 mM, slight activation of isoenzymes I and III | Metroxylon sagu | |
| 1.14.18.1 | Zn2+ | 0.9 mM, approx. 2fold activation of isoenzymes I and III | Metroxylon sagu | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 1.14.18.1 | 40000 | - |
isoenzymes I and III, gel filtration | Metroxylon sagu |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.14.18.1 | Metroxylon sagu | - |
sago palm, isoenzymes I and III | - |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 1.14.18.1 | isoenzyme I, hydroxylapatite, DEAE-Cellulose, Sephadex G-100, isoenzyme III, hydroxylapatite, Sephadex G-100 | Metroxylon sagu |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 1.14.18.1 | pith | - |
Metroxylon sagu | - |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.14.18.1 | 4-methylcatechol + O2 | - |
Metroxylon sagu | 4-methyl-o-benzoquinone + H2O | - |
? | |
| 1.14.18.1 | catechol + 1/2 O2 | - |
Metroxylon sagu | 1,2-benzoquinone + H2O | - |
? | |
| 1.14.18.1 | chlorogenic acid + O2 | - |
Metroxylon sagu | chlorogenoquinone + H2O | - |
? | |
| 1.14.18.1 | D-catechin + O2 | - |
Metroxylon sagu | ? | - |
? | |
| 1.14.18.1 | DL-epicatechin + O2 | - |
Metroxylon sagu | ? | - |
? | |
| 1.14.18.1 | epicatechin + O2 | - |
Metroxylon sagu | ? | - |
? | |
| 1.14.18.1 | L-epicatechin + O2 | - |
Metroxylon sagu | ? | - |
? | |
| 1.14.18.1 | L-tyrosine + O2 + AH2 | no activity with tyrosine | Metroxylon sagu | L-dopa + H2O + A | - |
? | |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.14.18.1 | 35 | - |
isoenzyme I | Metroxylon sagu |
| 1.14.18.1 | 45 | - |
isoenzyme III | Metroxylon sagu |
Temperature Range [°C]
| EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.14.18.1 | 25 | 65 | 25°C: about 50% of maximal activity, 65°C: about 45% of maximal activity, polyphenol oxidase I and II | Metroxylon sagu |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.14.18.1 | 40 | - |
inactivation of isozyme PPO I | Metroxylon sagu |
| 1.14.18.1 | 50 | - |
gradual inactivation of isozyme PPO III | Metroxylon sagu |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.14.18.1 | 6.5 | - |
isoenzymes I and III | Metroxylon sagu |
pH Range
| EC Number | pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.14.18.1 | 4.5 | 8 | pH 4.5: about 70% of maximal activity, pH 8.0: about 50% of maximal activity, negligible activity below pH 4 and above pH 9, isoenzyme PPO I | Metroxylon sagu |
| 1.14.18.1 | 5 | 8 | pH 5.0: about 30% of maximal activity, pH 8.0: about 50% of maximal activity, negligible activity below pH 4 and above pH 9, isoenzyme PPO II | Metroxylon sagu |
pH Stability
| EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.14.18.1 | 3 | 8 | isozyme PPO III, 90% activity after 18 h at pH 3.0 | Metroxylon sagu |
| 1.14.18.1 | 5 | 10 | isozyme PPO I, 90% activity after 18 h at pH 10.0 | Metroxylon sagu |
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