BRENDA - Enzyme Database

Partial purification and some properties of polyphenoloxidases from sago palm

Okamoto, A.; Imagawa, H.; Arai, Y.; Ozawa, T.; Agric. Biol. Chem. 52, 2215-2222 (1988)
No PubMed abstract available

Data extracted from this reference:

Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
1.14.18.1
CN-
0.2 mM, 50% inhibition of isoenzyme I, 0.13 mM, 50% inhibition of isoenzyme III
Metroxylon sagu
1.14.18.1
diethyldithiocarbamate
0.5 mM, 50% inhibition of isoenzyme I, 0.19 mM, 50% inhibition of isoenzyme III
Metroxylon sagu
1.14.18.1
EDTA
no inhibition EDTA
Metroxylon sagu
1.14.18.1
Sodium sulfate
-
Metroxylon sagu
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.14.18.1
3
-
D-catechin
isoenzyme III
Metroxylon sagu
1.14.18.1
3.03
-
DL-epicatechin
isoenzyme I
Metroxylon sagu
1.14.18.1
3.22
-
D-epicatechin
isoenzyme III
Metroxylon sagu
1.14.18.1
3.85
-
L-epicatechin
isoenzyme I
Metroxylon sagu
1.14.18.1
5
-
D-catechin
isoenzyme I
Metroxylon sagu
1.14.18.1
5.26
-
DL-epicatechin
isoenzyme III
Metroxylon sagu
1.14.18.1
6.67
-
D-epicatechin
isoenzyme I
Metroxylon sagu
1.14.18.1
11.1
-
L-epicatechin
isoenzyme III
Metroxylon sagu
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
1.14.18.1
Co2+
0.9 mM, slight activation of isoenzymes I and III
Metroxylon sagu
1.14.18.1
copper
0.1 mM, approx. 3fold activation of isoenzymes I and III
Metroxylon sagu
1.14.18.1
Mn2+
slight activation of isoenzymes I and III
Metroxylon sagu
1.14.18.1
Ni2+
0.9 mM, slight activation of isoenzymes I and III
Metroxylon sagu
1.14.18.1
Zn2+
0.9 mM, approx. 2fold activation of isoenzymes I and III
Metroxylon sagu
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.14.18.1
40000
-
isoenzymes I and III, gel filtration
Metroxylon sagu
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.14.18.1
Metroxylon sagu
-
sago palm, isoenzymes I and III
-
Purification (Commentary)
EC Number
Commentary
Organism
1.14.18.1
isoenzyme I, hydroxylapatite, DEAE-Cellulose, Sephadex G-100, isoenzyme III, hydroxylapatite, Sephadex G-100
Metroxylon sagu
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
1.14.18.1
pith
-
Metroxylon sagu
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.14.18.1
4-methylcatechol + O2
-
636356
Metroxylon sagu
4-methyl-o-benzoquinone + H2O
-
636356
Metroxylon sagu
?
1.14.18.1
catechol + 1/2 O2
-
636356
Metroxylon sagu
1,2-benzoquinone + H2O
-
636356
Metroxylon sagu
?
1.14.18.1
chlorogenic acid + O2
-
636356
Metroxylon sagu
chlorogenoquinone + H2O
-
636356
Metroxylon sagu
?
1.14.18.1
D-catechin + O2
-
636356
Metroxylon sagu
?
-
-
-
?
1.14.18.1
DL-epicatechin + O2
-
636356
Metroxylon sagu
?
-
-
-
?
1.14.18.1
epicatechin + O2
-
636356
Metroxylon sagu
?
-
-
-
-
1.14.18.1
L-epicatechin + O2
-
636356
Metroxylon sagu
?
-
-
-
?
1.14.18.1
L-tyrosine + O2 + AH2
no activity with tyrosine
636356
Metroxylon sagu
L-dopa + H2O + A
-
-
-
-
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
1.14.18.1
35
-
isoenzyme I
Metroxylon sagu
1.14.18.1
45
-
isoenzyme III
Metroxylon sagu
Temperature Range [°C]
EC Number
Temperature Minimum [°C]
Temperature Maximum [°C]
Commentary
Organism
1.14.18.1
25
65
25°C: about 50% of maximal activity, 65°C: about 45% of maximal activity, polyphenol oxidase I and II
Metroxylon sagu
Temperature Stability [°C]
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
1.14.18.1
40
-
inactivation of isozyme PPO I
Metroxylon sagu
1.14.18.1
50
-
gradual inactivation of isozyme PPO III
Metroxylon sagu
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.14.18.1
6.5
-
isoenzymes I and III
Metroxylon sagu
pH Range
EC Number
pH Minimum
pH Maximum
Commentary
Organism
1.14.18.1
4.5
8
pH 4.5: about 70% of maximal activity, pH 8.0: about 50% of maximal activity, negligible activity below pH 4 and above pH 9, isoenzyme PPO I
Metroxylon sagu
1.14.18.1
5
8
pH 5.0: about 30% of maximal activity, pH 8.0: about 50% of maximal activity, negligible activity below pH 4 and above pH 9, isoenzyme PPO II
Metroxylon sagu
pH Stability
EC Number
pH Stability
pH Stability Maximum
Commentary
Organism
1.14.18.1
3
8
isozyme PPO III, 90% activity after 18 h at pH 3.0
Metroxylon sagu
1.14.18.1
5
10
isozyme PPO I, 90% activity after 18 h at pH 10.0
Metroxylon sagu
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
1.14.18.1
CN-
0.2 mM, 50% inhibition of isoenzyme I, 0.13 mM, 50% inhibition of isoenzyme III
Metroxylon sagu
1.14.18.1
diethyldithiocarbamate
0.5 mM, 50% inhibition of isoenzyme I, 0.19 mM, 50% inhibition of isoenzyme III
Metroxylon sagu
1.14.18.1
EDTA
no inhibition EDTA
Metroxylon sagu
1.14.18.1
Sodium sulfate
-
Metroxylon sagu
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.14.18.1
3
-
D-catechin
isoenzyme III
Metroxylon sagu
1.14.18.1
3.03
-
DL-epicatechin
isoenzyme I
Metroxylon sagu
1.14.18.1
3.22
-
D-epicatechin
isoenzyme III
Metroxylon sagu
1.14.18.1
3.85
-
L-epicatechin
isoenzyme I
Metroxylon sagu
1.14.18.1
5
-
D-catechin
isoenzyme I
Metroxylon sagu
1.14.18.1
5.26
-
DL-epicatechin
isoenzyme III
Metroxylon sagu
1.14.18.1
6.67
-
D-epicatechin
isoenzyme I
Metroxylon sagu
1.14.18.1
11.1
-
L-epicatechin
isoenzyme III
Metroxylon sagu
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
1.14.18.1
Co2+
0.9 mM, slight activation of isoenzymes I and III
Metroxylon sagu
1.14.18.1
copper
0.1 mM, approx. 3fold activation of isoenzymes I and III
Metroxylon sagu
1.14.18.1
Mn2+
slight activation of isoenzymes I and III
Metroxylon sagu
1.14.18.1
Ni2+
0.9 mM, slight activation of isoenzymes I and III
Metroxylon sagu
1.14.18.1
Zn2+
0.9 mM, approx. 2fold activation of isoenzymes I and III
Metroxylon sagu
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.14.18.1
40000
-
isoenzymes I and III, gel filtration
Metroxylon sagu
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
1.14.18.1
isoenzyme I, hydroxylapatite, DEAE-Cellulose, Sephadex G-100, isoenzyme III, hydroxylapatite, Sephadex G-100
Metroxylon sagu
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
1.14.18.1
pith
-
Metroxylon sagu
-
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.14.18.1
4-methylcatechol + O2
-
636356
Metroxylon sagu
4-methyl-o-benzoquinone + H2O
-
636356
Metroxylon sagu
?
1.14.18.1
catechol + 1/2 O2
-
636356
Metroxylon sagu
1,2-benzoquinone + H2O
-
636356
Metroxylon sagu
?
1.14.18.1
chlorogenic acid + O2
-
636356
Metroxylon sagu
chlorogenoquinone + H2O
-
636356
Metroxylon sagu
?
1.14.18.1
D-catechin + O2
-
636356
Metroxylon sagu
?
-
-
-
?
1.14.18.1
DL-epicatechin + O2
-
636356
Metroxylon sagu
?
-
-
-
?
1.14.18.1
epicatechin + O2
-
636356
Metroxylon sagu
?
-
-
-
-
1.14.18.1
L-epicatechin + O2
-
636356
Metroxylon sagu
?
-
-
-
?
1.14.18.1
L-tyrosine + O2 + AH2
no activity with tyrosine
636356
Metroxylon sagu
L-dopa + H2O + A
-
-
-
-
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
1.14.18.1
35
-
isoenzyme I
Metroxylon sagu
1.14.18.1
45
-
isoenzyme III
Metroxylon sagu
Temperature Range [°C] (protein specific)
EC Number
Temperature Minimum [°C]
Temperature Maximum [°C]
Commentary
Organism
1.14.18.1
25
65
25°C: about 50% of maximal activity, 65°C: about 45% of maximal activity, polyphenol oxidase I and II
Metroxylon sagu
Temperature Stability [°C] (protein specific)
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
1.14.18.1
40
-
inactivation of isozyme PPO I
Metroxylon sagu
1.14.18.1
50
-
gradual inactivation of isozyme PPO III
Metroxylon sagu
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.14.18.1
6.5
-
isoenzymes I and III
Metroxylon sagu
pH Range (protein specific)
EC Number
pH Minimum
pH Maximum
Commentary
Organism
1.14.18.1
4.5
8
pH 4.5: about 70% of maximal activity, pH 8.0: about 50% of maximal activity, negligible activity below pH 4 and above pH 9, isoenzyme PPO I
Metroxylon sagu
1.14.18.1
5
8
pH 5.0: about 30% of maximal activity, pH 8.0: about 50% of maximal activity, negligible activity below pH 4 and above pH 9, isoenzyme PPO II
Metroxylon sagu
pH Stability (protein specific)
EC Number
pH Stability
pH Stability Maximum
Commentary
Organism
1.14.18.1
3
8
isozyme PPO III, 90% activity after 18 h at pH 3.0
Metroxylon sagu
1.14.18.1
5
10
isozyme PPO I, 90% activity after 18 h at pH 10.0
Metroxylon sagu