BRENDA - Enzyme Database

N-acetyl-6-hydroxytryptophan oxidase, a developmentally controlled phenol oxidase from Aspergillus nidulans

Birse, C.E.; Clutterbuck, A.J.; J. Gen. Microbiol. 136, 1725-1730 (1990)

Data extracted from this reference:

Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
1.14.18.1
1-Phenyl-2-thiourea
1 mM, 94% inhibition
Aspergillus nidulans
1.14.18.1
8-hydroxyquinoline
3 mM, 44% inhibition
Aspergillus nidulans
1.14.18.1
azide
no inhibition by sodium azide
Aspergillus nidulans
1.14.18.1
diethyldithiocarbamate
0.1 mM, complete inhibition
Aspergillus nidulans
1.14.18.1
Metabisulfite
0.1 mM, 99% inhibition
Aspergillus nidulans
1.14.18.1
o-phenanthroline hydrate
3 mM, 30% inhibition
Aspergillus nidulans
1.14.18.1
phenylhydrazine
1 mM, 86% inhibition, noncompetitive inhibition
Aspergillus nidulans
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.14.18.1
2
14
Hydroquinone monomethylether
range of estimated values for crude or purified extracts
Aspergillus nidulans
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
1.14.18.1
extracellular
-
Aspergillus nidulans
-
-
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
1.14.18.1
copper
2.1 copper atoms per polypeptide, enzyme form B
Aspergillus nidulans
1.14.18.1
Zn2+
0.9 mol per polypeptide
Aspergillus nidulans
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.14.18.1
48000
-
x * 48000, enzyme form B, SDS-PAGE
Aspergillus nidulans
1.14.18.1
50000
-
x * 50000, enzyme form A, SDS-PAGE
Aspergillus nidulans
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.14.18.1
N-acetyl-6-hydroxytryptophan + O2
Aspergillus nidulans
-
?
-
-
-
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.14.18.1
Aspergillus nidulans
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
1.14.18.1
DEAE-Sepharose, Phenyl Sepharose, enzyme form A and B
Aspergillus nidulans
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
1.14.18.1
conidiophore
-
Aspergillus nidulans
-
1.14.18.1
culture filtrate
-
Aspergillus nidulans
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [Ámol/min/mg]
Specific Activity Maximum [Ámol/min/mg]
Commentary
Organism
1.14.18.1
additional information
-
enzyme form A, 480.0 DELTA absorbance 470 nm/min/mg, enzyme form B, 258.6 DELTA absorbance 470 nm/min/mg
Aspergillus nidulans
Storage Stability
EC Number
Storage Stability
Organism
1.14.18.1
4░C, 3 weeks, no loss of activity
Aspergillus nidulans
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.14.18.1
4-methylcatechol + O2
-
636338
Aspergillus nidulans
4-methyl-o-benzoquinone + H2O
-
636338
Aspergillus nidulans
?
1.14.18.1
catechol + 1/2 O2
-
636338
Aspergillus nidulans
1,2-benzoquinone + H2O
-
636338
Aspergillus nidulans
?
1.14.18.1
chlorogenic acid + O2
-
636338
Aspergillus nidulans
chlorogenoquinone + H2O
-
636338
Aspergillus nidulans
?
1.14.18.1
D-catechin + O2
-
636338
Aspergillus nidulans
?
-
-
-
?
1.14.18.1
hydroquinone monomethylether + O2
-
636338
Aspergillus nidulans
quinone monomethylether
-
636338
Aspergillus nidulans
?
1.14.18.1
N-acetyl-6-hydroxytryptophan + O2
-
636338
Aspergillus nidulans
?
-
-
-
-
1.14.18.1
N-acetyl-6-hydroxytryptophan + O2
-
636338
Aspergillus nidulans
?
-
636338
Aspergillus nidulans
?
1.14.18.1
o-methoxyphenol + O2 + AH2
trivial name guaiacol
636338
Aspergillus nidulans
1,2-dihydroxy-3-methoxybenzene + H2O + A
-
636338
Aspergillus nidulans
?
Subunits
EC Number
Subunits
Commentary
Organism
1.14.18.1
?
x * 48000, enzyme form B, SDS-PAGE; x * 50000, enzyme form A, SDS-PAGE
Aspergillus nidulans
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.14.18.1
7
-
with hydroquinone monomethylether
Aspergillus nidulans
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
1.14.18.1
1-Phenyl-2-thiourea
1 mM, 94% inhibition
Aspergillus nidulans
1.14.18.1
8-hydroxyquinoline
3 mM, 44% inhibition
Aspergillus nidulans
1.14.18.1
azide
no inhibition by sodium azide
Aspergillus nidulans
1.14.18.1
diethyldithiocarbamate
0.1 mM, complete inhibition
Aspergillus nidulans
1.14.18.1
Metabisulfite
0.1 mM, 99% inhibition
Aspergillus nidulans
1.14.18.1
o-phenanthroline hydrate
3 mM, 30% inhibition
Aspergillus nidulans
1.14.18.1
phenylhydrazine
1 mM, 86% inhibition, noncompetitive inhibition
Aspergillus nidulans
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.14.18.1
2
14
Hydroquinone monomethylether
range of estimated values for crude or purified extracts
Aspergillus nidulans
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
1.14.18.1
extracellular
-
Aspergillus nidulans
-
-
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
1.14.18.1
copper
2.1 copper atoms per polypeptide, enzyme form B
Aspergillus nidulans
1.14.18.1
Zn2+
0.9 mol per polypeptide
Aspergillus nidulans
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.14.18.1
48000
-
x * 48000, enzyme form B, SDS-PAGE
Aspergillus nidulans
1.14.18.1
50000
-
x * 50000, enzyme form A, SDS-PAGE
Aspergillus nidulans
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.14.18.1
N-acetyl-6-hydroxytryptophan + O2
Aspergillus nidulans
-
?
-
-
-
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
1.14.18.1
DEAE-Sepharose, Phenyl Sepharose, enzyme form A and B
Aspergillus nidulans
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
1.14.18.1
conidiophore
-
Aspergillus nidulans
-
1.14.18.1
culture filtrate
-
Aspergillus nidulans
-
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [Ámol/min/mg]
Specific Activity Maximum [Ámol/min/mg]
Commentary
Organism
1.14.18.1
additional information
-
enzyme form A, 480.0 DELTA absorbance 470 nm/min/mg, enzyme form B, 258.6 DELTA absorbance 470 nm/min/mg
Aspergillus nidulans
Storage Stability (protein specific)
EC Number
Storage Stability
Organism
1.14.18.1
4░C, 3 weeks, no loss of activity
Aspergillus nidulans
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.14.18.1
4-methylcatechol + O2
-
636338
Aspergillus nidulans
4-methyl-o-benzoquinone + H2O
-
636338
Aspergillus nidulans
?
1.14.18.1
catechol + 1/2 O2
-
636338
Aspergillus nidulans
1,2-benzoquinone + H2O
-
636338
Aspergillus nidulans
?
1.14.18.1
chlorogenic acid + O2
-
636338
Aspergillus nidulans
chlorogenoquinone + H2O
-
636338
Aspergillus nidulans
?
1.14.18.1
D-catechin + O2
-
636338
Aspergillus nidulans
?
-
-
-
?
1.14.18.1
hydroquinone monomethylether + O2
-
636338
Aspergillus nidulans
quinone monomethylether
-
636338
Aspergillus nidulans
?
1.14.18.1
N-acetyl-6-hydroxytryptophan + O2
-
636338
Aspergillus nidulans
?
-
-
-
-
1.14.18.1
N-acetyl-6-hydroxytryptophan + O2
-
636338
Aspergillus nidulans
?
-
636338
Aspergillus nidulans
?
1.14.18.1
o-methoxyphenol + O2 + AH2
trivial name guaiacol
636338
Aspergillus nidulans
1,2-dihydroxy-3-methoxybenzene + H2O + A
-
636338
Aspergillus nidulans
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
1.14.18.1
?
x * 48000, enzyme form B, SDS-PAGE; x * 50000, enzyme form A, SDS-PAGE
Aspergillus nidulans
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.14.18.1
7
-
with hydroquinone monomethylether
Aspergillus nidulans