BRENDA - Enzyme Database

Polyphenoloxidase from apple, partial purification and some properties

Janovitz-Klapp, A.; Richard, F.; Nicholas, J.; Phytochemistry 28, 2903-2907 (1989)
No PubMed abstract available

Data extracted from this reference:

KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.14.18.1
4.1
-
chlorogenic acid
at pH 4.0, slight increase in Km above pH 5.0
Malus sp.
1.14.18.1
4.9
-
chlorogenic acid
at pH 5.5, slight increase in Km above pH 5.0
Malus sp.
1.14.18.1
5.2
-
4-methylcatechol
at pH 3.5-5.0, strong increase in Km above pH 5.0
Malus sp.
1.14.18.1
6.1
-
(+)-catechin
at pH 4.0, slight increase in Km above pH 4.0
Malus sp.
1.14.18.1
6.6
-
(+)-catechin
at pH 5.5, slight increase in Km above pH 4.0
Malus sp.
1.14.18.1
20.5
-
4-methylcatechol
at pH 6.3, strong increase in Km above pH 5.0
Malus sp.
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.14.18.1
46000
-
gel filtration
Malus sp.
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.14.18.1
Malus sp.
-
apple, var. red delicious
-
Purification (Commentary)
EC Number
Commentary
Organism
1.14.18.1
ammonium sulfate, Phenyl Sepharose
Malus sp.
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
1.14.18.1
fruit
peel and cortex
Malus sp.
-
1.14.18.1
peel
-
Malus sp.
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.14.18.1
11330
-
-
Malus sp.
Storage Stability
EC Number
Storage Stability
Organism
1.14.18.1
4°C, 15 days, 10% loss of activity
Malus sp.
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.14.18.1
4-methylcatechol + O2
-
636336
Malus sp.
4-methyl-o-benzoquinone + H2O
-
636336
Malus sp.
?
1.14.18.1
chlorogenic acid + O2
-
636336
Malus sp.
chlorogenoquinone + H2O
-
636336
Malus sp.
?
1.14.18.1
D-catechin + O2
-
636336
Malus sp.
?
-
-
-
-
Temperature Stability [°C]
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
1.14.18.1
30
-
4 h, pH 4.5, 50% loss of activity
Malus sp.
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.14.18.1
4.5
5
with 4-methylcatechol, chlorogenic acid and (+)-catechin
Malus sp.
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.14.18.1
4.1
-
chlorogenic acid
at pH 4.0, slight increase in Km above pH 5.0
Malus sp.
1.14.18.1
4.9
-
chlorogenic acid
at pH 5.5, slight increase in Km above pH 5.0
Malus sp.
1.14.18.1
5.2
-
4-methylcatechol
at pH 3.5-5.0, strong increase in Km above pH 5.0
Malus sp.
1.14.18.1
6.1
-
(+)-catechin
at pH 4.0, slight increase in Km above pH 4.0
Malus sp.
1.14.18.1
6.6
-
(+)-catechin
at pH 5.5, slight increase in Km above pH 4.0
Malus sp.
1.14.18.1
20.5
-
4-methylcatechol
at pH 6.3, strong increase in Km above pH 5.0
Malus sp.
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.14.18.1
46000
-
gel filtration
Malus sp.
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
1.14.18.1
ammonium sulfate, Phenyl Sepharose
Malus sp.
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
1.14.18.1
fruit
peel and cortex
Malus sp.
-
1.14.18.1
peel
-
Malus sp.
-
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.14.18.1
11330
-
-
Malus sp.
Storage Stability (protein specific)
EC Number
Storage Stability
Organism
1.14.18.1
4°C, 15 days, 10% loss of activity
Malus sp.
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.14.18.1
4-methylcatechol + O2
-
636336
Malus sp.
4-methyl-o-benzoquinone + H2O
-
636336
Malus sp.
?
1.14.18.1
chlorogenic acid + O2
-
636336
Malus sp.
chlorogenoquinone + H2O
-
636336
Malus sp.
?
1.14.18.1
D-catechin + O2
-
636336
Malus sp.
?
-
-
-
-
Temperature Stability [°C] (protein specific)
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
1.14.18.1
30
-
4 h, pH 4.5, 50% loss of activity
Malus sp.
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.14.18.1
4.5
5
with 4-methylcatechol, chlorogenic acid and (+)-catechin
Malus sp.