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Literature summary extracted from

  • Fujitsuka, N.; Yokozawa, T.; Oura, H.; Akao, T.; Kobashi, K.; Ienaga, K.; Nakamura, K.
    L-Gulono-gamma-lactone oxidase is the enzyme responsible for the production of methylguanidine in the rat liver (1993), Nephron, 63, 445-451.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.3.8 0.005
-
 L-gulono-1,4-lactone at pH 7.4 Rattus norvegicus
1.1.3.8 4.28
-
 creatol at pH 9.0 Rattus norvegicus
1.1.3.8 12.8
-
 creatol at pH 7.4 Rattus norvegicus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.1.3.8 microsome
-
 Rattus norvegicus
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.3.8 50000
-
 SDS-PAGE Rattus norvegicus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.3.8 L-gulono-1,4-lactone + O2 Rattus norvegicus
-
 L-xylo-hex-3-ulonolactone + H2O2
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.3.8 Rattus norvegicus
-
-
-

Reaction

EC Number Reaction Comment Organism Reaction ID
1.1.3.8 L-gulono-1,4-lactone + O2 = L-xylo-hex-2-ulono-1,4-lactone + H2O2 product isomerizes spontaneously to L-ascorbate Rattus norvegicus
a

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.3.8 liver
-
 Rattus norvegicus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.3.8 0.078
-
 methylguanidine synthesis Rattus norvegicus
1.1.3.8 1.11
-
 L-ascorbate synthesis Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.3.8 creatol + O2 creatone A and creatone B are intermediates Rattus norvegicus methylguanidine + ?
-
 ?
1.1.3.8 L-gulono-1,4-lactone + O2
-
 Rattus norvegicus L-xylo-hex-3-ulonolactone + H2O2
-
 ?
1.1.3.8 L-gulono-1,4-lactone + O2
-
 Rattus norvegicus L-xylo-hex-3-ulonolactone + H2O2 product isomerizes spontaneously to L-ascorbate ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.3.8 37
-
-
 Rattus norvegicus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.3.8 7.5
-
 L-ascorbate synthesis Rattus norvegicus
1.1.3.8 9
-
 synthesis of methylguanidine Rattus norvegicus

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.3.8 flavin covalently bound, flavoenzyme Rattus norvegicus