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Literature summary extracted from

  • Sugisawa, T.; Ojima, S.; Matzinger, P.K.; Hoshino, T.
    Isolation and characterization of a new vitamin C producing enzyme (L-gulono-gamma-lactone dehydrogenase) of bacterial origin (1995), Biosci. Biotechnol. Biochem., 59, 190-196.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.3.8 Cu(NO3)2 0.19 mM concentration 12% inhibition, 0.38 mM concentration 76% inhibition and 0.89 mM concentration 100% inhibition Gluconobacter oxydans
1.1.3.8 CuCl2 * 2H2O 0.19 mM concentration 16% inhibition, 0.38 mM concentration 92% inhibition and 0.89 mM concentration 100% inhibition Gluconobacter oxydans
1.1.3.8 CuSO4 0.19 mM concentration 20% inhibition, 0.38 mM concentration 80% inhibition and 0.89 mM concentration 100% inhibition Gluconobacter oxydans
1.1.3.8 Fe2(SO4)3 sub mM concentration slight inhibition Gluconobacter oxydans
1.1.3.8 MgCl2 * 6H2O sub mM concentration slight inhibition Gluconobacter oxydans
1.1.3.8 MgSO4 * 7H2O sub mM concentration slight inhibition Gluconobacter oxydans
1.1.3.8 MnCl2 * 4H2O 0.38 mM and 0.89 mM concentration 100% inhibition Gluconobacter oxydans
1.1.3.8 MnSO4 * 4-6H2O 0.38 mM and 0.89 mM concentration 100% inhibition Gluconobacter oxydans
1.1.3.8 additional information not inhibitory are: EDTA, sodium azide, monoiodoacetate, p-chloromercuribenzoate, NaHAsO4, sodium fluoride, KCN, hydroxyl ammonium sulfate, hydroxylamine, hydrazine monohydrate, hydrazinium dichloride, and 1,4-benzoquinone Gluconobacter oxydans
1.1.3.8 NiSO4 sub mM concentration slight inhibition Gluconobacter oxydans
1.1.3.8 TiCl2 sub mM concentration slight inhibition Gluconobacter oxydans
1.1.3.8 ZnCl2 sub mM concentration slight inhibition Gluconobacter oxydans
1.1.3.8 ZnSO4 sub mM concentration slight inhibition Gluconobacter oxydans

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.1.3.8 soluble
-
Gluconobacter oxydans
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.3.8 16500
-
alpha,beta,gamma, 1 * 61000 + 1 * 32500 + 1 * 16500, the largest is a flavoprotein, gel filtration with 0.1% SDS in buffer and SDS-PAGE Gluconobacter oxydans
1.1.3.8 32500
-
alpha,beta,gamma, 1 * 61000 + 1 * 32500 + 1 * 16500, the largest is a flavoprotein, gel filtration with 0.1% SDS in buffer and SDS-PAGE Gluconobacter oxydans
1.1.3.8 61000
-
alpha,beta,gamma, 1 * 61000 + 1 * 32500 + 1 * 16500, the largest is a flavoprotein, gel filtration with 0.1% SDS in buffer and SDS-PAGE Gluconobacter oxydans
1.1.3.8 110000
-
gel filtration Gluconobacter oxydans

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.3.8 L-gulono-1,4-lactone + O2 Gluconobacter oxydans
-
L-xylo-hex-3-ulonolactone + H2O2
-
?

Organism

EC Number Organism UniProt Comment Textmining
1.1.3.8 Gluconobacter oxydans
-
strain DSM 4025
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.3.8
-
Gluconobacter oxydans

Reaction

EC Number Reaction Comment Organism Reaction ID
1.1.3.8 L-gulono-1,4-lactone + O2 = L-xylo-hex-2-ulono-1,4-lactone + H2O2 product isomerizes spontaneously to L-ascorbate Gluconobacter oxydans

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.3.8 D-glucose + phenazine methosulfate + O2 23.4% as active as L-gulono-1,4-lactone Gluconobacter oxydans D-glucosone + ?
-
?
1.1.3.8 D-mannose + phenazine methosulfate + O2 6.4% as active as L-gulono-1,4-lactone Gluconobacter oxydans ?
-
?
1.1.3.8 D-xylose + phenazine methosulfate + O2 6.4% as active as L-gulono-1,4-lactone Gluconobacter oxydans D-xylosone + ?
-
?
1.1.3.8 L-glucurono-1,4-lactone + phenazine methosulfate + O2 6.4% as active as L-gulono-1,4-lactone Gluconobacter oxydans ?
-
?
1.1.3.8 L-gulono-1,4-lactone + 2,6-dichlorophenolindophenol + O2 enzyme is a dehydrogenase, not an oxidase Gluconobacter oxydans L-ascorbic acid + ?
-
?
1.1.3.8 L-gulono-1,4-lactone + O2
-
Gluconobacter oxydans L-xylo-hex-3-ulonolactone + H2O2
-
?
1.1.3.8 L-gulono-1,4-lactone + O2
-
Gluconobacter oxydans L-xylo-hex-3-ulonolactone + H2O2 product isomerizes spontaneously to L-ascorbate ?
1.1.3.8 L-gulono-1,4-lactone + phenazine methosulfate + O2 enzyme is a dehydrogenase, not an oxidase Gluconobacter oxydans L-ascorbic acid + ?
-
?

Subunits

EC Number Subunits Comment Organism
1.1.3.8 trimer alpha,beta,gamma, 1 * 61000 + 1 * 32500 + 1 * 16500, the largest is a flavoprotein, gel filtration with 0.1% SDS in buffer and SDS-PAGE Gluconobacter oxydans

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
1.1.3.8 25 55 no detectable optimum Gluconobacter oxydans

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.1.3.8
-
55 53% activity at 55 C Gluconobacter oxydans

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.3.8 7.5
-
-
Gluconobacter oxydans

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.1.3.8 6.5 9.2
-
Gluconobacter oxydans