Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Malaoui, H.; Marczak, R.
    Separation and characterization of the 1,3-propanediol and glycerol dehydrogenase activities from Clostridium butyricum E5 wild-type and mutant D (2001), J. Appl. Microbiol., 90, 1006-1014.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.6 0.08
-
 NADH pH 9.1, 37°C, wild-type enzyme Clostridium butyricum
1.1.1.6 0.12
-
 NADH pH 9.1, 37°C, mutant D Clostridium butyricum
1.1.1.6 1.18
-
 dihydroxyacetone pH 9.1, 37°C, wild-type enzyme Clostridium butyricum
1.1.1.6 1.18
-
 dihydroxyacetone wild type enzyme, at pH 9.1 and 37°C Clostridium butyricum
1.1.1.6 2
-
 dihydroxyacetone pH 9.1, 37°C, mutant D Clostridium butyricum
1.1.1.6 2
-
 dihydroxyacetone mutant enzyme E5-MD, at pH 9.1 and 37°C Clostridium butyricum
1.1.1.6 4.07
-
 NAD+ pH 9.7, 37°C, wild-type enzyme Clostridium butyricum
1.1.1.6 4.7
-
 NAD+ pH 9.7, 37°C, mutant D Clostridium butyricum
1.1.1.6 73.3
-
 glycerol pH 9.7, 37°C, mutant D 76.3 mM Clostridium butyricum
1.1.1.6 91.7
-
 glycerol pH 9.7, 37°C, wild-type enzyme Clostridium butyricum

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.1.1.6 cytoplasm soluble, isolation from crude cell extract Clostridium butyricum 5737
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.1.1.6 Ca2+ 1 mM, stimulates 64.5% compared to 1 mM Mn2+ Clostridium butyricum
1.1.1.6 Fe2+ 1 mM, stimulates 26.3% compared to 1 mM Mn2+ Clostridium butyricum
1.1.1.6 Mn2+ 1 mM, activates Clostridium butyricum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.6 181000
-
 gel filtration Clostridium butyricum

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.6 Clostridium butyricum
-
 mutant D
-
1.1.1.6 Clostridium butyricum E5
-
 mutant D
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.6
-
 Clostridium butyricum

Source Tissue

EC Number Source Tissue Comment Organism Textmining

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.6 1,3-propanediol + NAD+
-
 Clostridium butyricum ?
-
 ?
1.1.1.6 1,3-propanediol + NAD+
-
 Clostridium butyricum E5 ?
-
 ?
1.1.1.6 2,3-butanediol + NAD+
-
 Clostridium butyricum 3-hydroxybutane-2-one + NADH
-
 ?
1.1.1.6 2,3-butanediol + NAD+
-
 Clostridium butyricum E5 3-hydroxybutane-2-one + NADH
-
 ?
1.1.1.6 3-hydroxypropionaldehyde + NADH
-
 Clostridium butyricum propan-1,3-diol + NAD+
-
 ?
1.1.1.6 acetaldehyde + NADH + H+
-
 Clostridium butyricum ethanol + NAD+
-
 ?
1.1.1.6 acetaldehyde + NADH + H+
-
 Clostridium butyricum E5 ethanol + NAD+
-
 ?
1.1.1.6 dihydroxyacetone + NADH + H+
-
 Clostridium butyricum glycerol + NAD+
-
 r
1.1.1.6 DL-glyceraldehyde + NADH
-
 Clostridium butyricum glycerol + NAD+
-
 ?
1.1.1.6 DL-glyceraldehyde + NADH
-
 Clostridium butyricum E5 glycerol + NAD+
-
 ?
1.1.1.6 N-butyraldehyde + NADH
-
 Clostridium butyricum 1-butanol + NAD+
-
 ?
1.1.1.6 propionaldehyde + NADH
-
 Clostridium butyricum 1-propanol + NAD+
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.6 7.2
-
 oxidation of glycerol, wild-type enzyme Clostridium butyricum
1.1.1.6 7.5
-
 oxidation of glycerol, mutant D Clostridium butyricum
1.1.1.6 8.3
-
 reduction of dihydroxyacetone, mutant D Clostridium butyricum
1.1.1.6 8.6
-
 reduction of dihydroxyacetone, wild-type enzyme Clostridium butyricum