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Literature summary extracted from

  • Scharschmidt, M.; Pfleiderer, G.; Metz, H.; Brummer, W.
    Isolierung und Charakterisierung von Glycerol-Dehydrogenase aus Bacillus megaterium (1983), Hoppe-Seyler's Z. Physiol. Chem., 364, 911-921.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.6 1,10-phenanthroline
-
 Priestia megaterium
1.1.1.6 2,2'-bipyridyl
-
 Priestia megaterium
1.1.1.6 3,4-dimercaptotoluol
-
 Priestia megaterium
1.1.1.6 8-hydroxyquinoline
-
 Priestia megaterium
1.1.1.6 diphosphate
-
 Priestia megaterium
1.1.1.6 EDTA
-
 Priestia megaterium
1.1.1.6 Imidazol
-
 Priestia megaterium
1.1.1.6 pyridin-2,6-dicarboxylic acid
-
 Priestia megaterium

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.6 0.3
-
 NAD+ pH 9.0, 25°C Priestia megaterium
1.1.1.6 1.4
-
 glycerol pH 9.0, 25°C Priestia megaterium

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.1.1.6 cytoplasm soluble, isolation from crude cell extract Priestia megaterium 5737
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.1.1.6 Zn2+ 1.6 mol Zn2+ bound per subunit Priestia megaterium

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.6 38000
-
 4 * 38000, SDS-PAGE Priestia megaterium
1.1.1.6 156000 160000 density gradient centrifugation, gel filtration Priestia megaterium

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.6 glycerol + NAD+ Priestia megaterium
-
 dihydroxyacetone + NADH + H+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.6 Priestia megaterium
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.6
-
 Priestia megaterium

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.6 58.1
-
-
 Priestia megaterium

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.6 1,2-propanediol + NAD+
-
 Priestia megaterium hydroxyacetone + NADH
-
 ?
1.1.1.6 erythrite + NAD+
-
 Priestia megaterium ?
-
 ?
1.1.1.6 glycerol + NAD+
-
 Priestia megaterium dihydroxyacetone + NADH + H+
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.1.6 tetramer 4 * 38000, SDS-PAGE Priestia megaterium

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.1.1.6 70
-
 30 min stable Priestia megaterium

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.6 9
-
-
 Priestia megaterium

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.6 NAD+
-
 Priestia megaterium
1.1.1.6 NADH
-
 Priestia megaterium