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Literature summary extracted from

  • Mai, X.; Adams, M.W.W.
    Purification and characterization of two reversible and ADP-dependent acetyl coenzyme A synthetases from the hyperthermophilic archaeon Pyrococcus furiosus (1996), J. Bacteriol., 178, 5897-5903.
    View publication on PubMedView publication on EuropePMC

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.2.1.13 0.004
-
 phenylacetyl-CoA isoenzyme ACS II Pyrococcus furiosus
6.2.1.13 0.012
-
 isobutyryl-CoA isoenzyme ACS II Pyrococcus furiosus
6.2.1.13 0.018
-
 CoA isoenzyme ACS I Pyrococcus furiosus
6.2.1.13 0.025
-
 acetyl-CoA isoenzyme ACS I Pyrococcus furiosus
6.2.1.13 0.026
-
 acetyl-CoA isoenzyme ACS II Pyrococcus furiosus
6.2.1.13 0.029
-
 isobutyryl-CoA isoenzyme ACS I Pyrococcus furiosus
6.2.1.13 0.061
-
 ADP isoenzyme ACS II Pyrococcus furiosus
6.2.1.13 0.132
-
 GDP isoenzyme ACS I Pyrococcus furiosus
6.2.1.13 0.15
-
 ADP isoenzyme ACS I Pyrococcus furiosus
6.2.1.13 0.236
-
 GDP isoenzyme ACS II Pyrococcus furiosus
6.2.1.13 0.396
-
 phosphate isoenzyme ACS I Pyrococcus furiosus
6.2.1.13 0.43
-
 GTP isoenzyme ACS I Pyrococcus furiosus
6.2.1.13 0.457
-
 Isobutyrate isoenzyme ACS I Pyrococcus furiosus
6.2.1.13 0.477
-
 ATP isoenzyme ACS I Pyrococcus furiosus
6.2.1.13 0.58
-
 phosphate isoenzyme ACS II Pyrococcus furiosus
6.2.1.13 1.1
-
 acetate isoenzyme ACS I Pyrococcus furiosus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
6.2.1.13 additional information contains neither iron nor other metals, such as copper, zinc, or magnesium Pyrococcus furiosus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
6.2.1.13 23000
-
 2 * 45000 (alpha) + 2 * 23000 (beta), SDS-PAGE Pyrococcus furiosus
6.2.1.13 45000
-
 2 * 45000 (alpha) + 2 * 23000 (beta), SDS-PAGE Pyrococcus furiosus
6.2.1.13 140000
-
 gel filtration Pyrococcus furiosus

Organism

EC Number Organism UniProt Comment Textmining
6.2.1.13 Pyrococcus furiosus
-
 2 isoenzymes: ACS I and ACS II
-

Oxidation Stability

EC Number Oxidation Stability Organism
6.2.1.13 stable to oxygen for 24 h Pyrococcus furiosus

Purification (Commentary)

EC Number Purification (Comment) Organism
6.2.1.13 isoenzymes ACS I and ACS II Pyrococcus furiosus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
6.2.1.13 30
-
 isoenzyme ACS II Pyrococcus furiosus
6.2.1.13 64.6
-
 isoenzyme ACS I Pyrococcus furiosus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.2.1.13 ADP + phosphate + acetyl-CoA r Pyrococcus furiosus ATP + acetate + CoA
-
 ?
6.2.1.13 ADP + phosphate + acetyl-CoA isoenzymes ACS I and ACS II Pyrococcus furiosus ATP + acetate + CoA
-
 ?
6.2.1.13 ADP + phosphate + indoleacetyl-CoA r, isoenzyme ACS II is active, ACS I not Pyrococcus furiosus ATP + indoleacetate + CoA
-
 ?
6.2.1.13 ADP + phosphate + phenylacetyl-CoA r, isoenzyme ACS II is active, ACS I not Pyrococcus furiosus ATP + phenylacetate + CoA
-
 ?
6.2.1.13 ADP + phosphate + succinyl-CoA no activity with isoenzymes ACS I and ACS II Pyrococcus furiosus ATP + succinate + CoA
-
 ?
6.2.1.13 ATP + isobutyrate + CoA r Pyrococcus furiosus ADP + phosphate + isobutyryl-CoA
-
 ?
6.2.1.13 ATP + isobutyrate + CoA isoenzymes ACS I and ACS II Pyrococcus furiosus ADP + phosphate + isobutyryl-CoA
-
 ?
6.2.1.13 GDP + phosphate + acetyl-CoA
-
 Pyrococcus furiosus GTP + acetate + CoA
-
 ?

Subunits

EC Number Subunits Comment Organism
6.2.1.13 tetramer 2 * 45000 (alpha) + 2 * 23000 (beta), SDS-PAGE Pyrococcus furiosus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
6.2.1.13 90
-
 above, at pH 8.0 Pyrococcus furiosus

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
6.2.1.13 25
-
 isoenzymes ACS I and ACS II are inactive at Pyrococcus furiosus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
6.2.1.13 80
-
 t1/2: 18 h for isoenzyme ACS I, 8 h for isoenzyme ACS II Pyrococcus furiosus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
6.2.1.13 8
-
 isobutyryl-CoA isoenzyme ACS II Pyrococcus furiosus
6.2.1.13 21
-
 GDP isoenzyme ACS II Pyrococcus furiosus
6.2.1.13 22
-
 Isobutyrate isoenzyme ACS II Pyrococcus furiosus
6.2.1.13 27
-
 GTP isoenzyme ACS II Pyrococcus furiosus
6.2.1.13 42
-
 acetyl-CoA isoenzyme ACS II Pyrococcus furiosus
6.2.1.13 55
-
 Isobutyrate isoenzyme ACS I Pyrococcus furiosus
6.2.1.13 65
-
 CoA isoenzyme ACS I Pyrococcus furiosus
6.2.1.13 66
-
 indoleacetate isoenzyme ACS II Pyrococcus furiosus
6.2.1.13 67
-
 acetate isoenzyme ACS II Pyrococcus furiosus
6.2.1.13 68
-
 ATP isoenzyme ACS II Pyrococcus furiosus
6.2.1.13 70
-
 CoA isoenzyme ACS II Pyrococcus furiosus
6.2.1.13 73
-
 CoA isoenzyme ACS I Pyrococcus furiosus
6.2.1.13 82
-
 ATP isoenzyme ACS I Pyrococcus furiosus
6.2.1.13 89
-
 phenylacetate isoenzyme ACS II Pyrococcus furiosus
6.2.1.13 115
-
 ADP isoenzyme ACS II Pyrococcus furiosus
6.2.1.13 117
-
 phosphate isoenzyme ACS II Pyrococcus furiosus
6.2.1.13 121
-
 isobutyryl-CoA GTP, isoenzyme ACS I Pyrococcus furiosus
6.2.1.13 138
-
 phenylacetyl-CoA isoenzyme ACS II Pyrococcus furiosus
6.2.1.13 157
-
 acetyl-CoA isoenzyme ACS I Pyrococcus furiosus
6.2.1.13 182
-
 phosphate isoenzyme ACS I Pyrococcus furiosus
6.2.1.13 203
-
 ADP isoenzyme ACS I Pyrococcus furiosus
6.2.1.13 411
-
 GDP isoenzyme ACS I Pyrococcus furiosus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
6.2.1.13 9
-
 at 80°C Pyrococcus furiosus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
6.2.1.13 6 11 6: about 60% of maximal activity, 11: about 40% of maximal activity, isoenzyme ACS I Pyrococcus furiosus
6.2.1.13 7 11 7: about 60% of maximal activity, 11: about 65% of maximal activity, isoenzyme ACS II Pyrococcus furiosus