BRENDA - Enzyme Database

The methylaspartase reaction probed using 2H- and 15N-isotope E ects for three substrates: A flip from a concerted to a carbocationic amino-enzyme elimination mechanism upon changing the C-3 stereochemistry in the substrate from R to S

Gani, D.; Archer, C.H.; Botting, N.P.; Pollard, J.R.; Bioorg. Med. Chem. 7, 977-990 (1999)

Data extracted from this reference:

Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
4.3.1.2
N-ethylmaleimide
irreversible
Clostridium tetanomorphum
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
4.3.1.2
K+
-
Clostridium tetanomorphum
4.3.1.2
Mg2+
-
Clostridium tetanomorphum
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
4.3.1.2
L-threo-3-methylaspartate
Clostridium tetanomorphum
-
mesaconate + NH3
-
Clostridium tetanomorphum
r
4.3.1.2
L-threo-3-methylaspartate
Clostridium tetanomorphum
-
?
-
-
-
4.3.1.2
L-threo-3-methylaspartate
Clostridium tetanomorphum H1 / ATCC 15920
-
mesaconate + NH3
-
Clostridium tetanomorphum H1 / ATCC 15920
r
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
4.3.1.2
Clostridium tetanomorphum
-
; ATCC 15920
-
4.3.1.2
Clostridium tetanomorphum H1 / ATCC 15920
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
4.3.1.2
-
Clostridium tetanomorphum
Reaction
EC Number
Reaction
Commentary
Organism
4.3.1.2
L-threo-3-methylaspartate = mesaconate + NH3
carbon mechanism; mechanism
Clostridium tetanomorphum
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4.3.1.2
(2S)-aspartate
-
6064
Clostridium tetanomorphum
fumarate + NH3
-
6064
Clostridium tetanomorphum
?
4.3.1.2
erythro-3-methylaspartate
(2S,3R)-3-methylaspartic acid
6064
Clostridium tetanomorphum
?
-
-
-
-
4.3.1.2
L-aspartate
(2S)-aspartate
6064
Clostridium tetanomorphum
fumarate + NH3
-
-
-
-
4.3.1.2
L-aspartate
(2S)-aspartate
6064
Clostridium tetanomorphum H1 / ATCC 15920
fumarate + NH3
-
-
-
-
4.3.1.2
L-erythro-3-methylaspartate
i.e. (2S,3R)-3-methylaspartate, slow syn elimination
6064
Clostridium tetanomorphum
mesaconate + NH3
-
6064
Clostridium tetanomorphum
?
4.3.1.2
L-threo-3-methylaspartate
-
6064
Clostridium tetanomorphum
mesaconate + NH3
-
6064
Clostridium tetanomorphum
r
4.3.1.2
L-threo-3-methylaspartate
r
6064
Clostridium tetanomorphum
mesaconate + NH3
-
6064
Clostridium tetanomorphum
-
4.3.1.2
L-threo-3-methylaspartate
(2S,3S)-3-methylaspartic acid
6064
Clostridium tetanomorphum
mesaconate + NH3
-
6064
Clostridium tetanomorphum
-
4.3.1.2
L-threo-3-methylaspartate
i.e. (2S,3S)-3-methylaspartate
6064
Clostridium tetanomorphum
mesaconate + NH3
-
6064
Clostridium tetanomorphum
r
4.3.1.2
L-threo-3-methylaspartate
-
6064
Clostridium tetanomorphum H1 / ATCC 15920
mesaconate + NH3
-
6064
Clostridium tetanomorphum H1 / ATCC 15920
r
4.3.1.2
L-threo-3-methylaspartate
r
6064
Clostridium tetanomorphum H1 / ATCC 15920
mesaconate + NH3
-
6064
Clostridium tetanomorphum H1 / ATCC 15920
-
4.3.1.2
L-threo-3-methylaspartate
(2S,3S)-3-methylaspartic acid
6064
Clostridium tetanomorphum H1 / ATCC 15920
mesaconate + NH3
-
6064
Clostridium tetanomorphum H1 / ATCC 15920
-
4.3.1.2
L-threo-3-methylaspartate
i.e. (2S,3S)-3-methylaspartate
6064
Clostridium tetanomorphum H1 / ATCC 15920
mesaconate + NH3
-
6064
Clostridium tetanomorphum H1 / ATCC 15920
r
4.3.1.2
L-threo-3-methylaspartate
-
6064
Clostridium tetanomorphum
?
-
-
-
-
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
4.3.1.2
N-ethylmaleimide
irreversible
Clostridium tetanomorphum
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
4.3.1.2
K+
-
Clostridium tetanomorphum
4.3.1.2
Mg2+
-
Clostridium tetanomorphum
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
4.3.1.2
L-threo-3-methylaspartate
Clostridium tetanomorphum
-
mesaconate + NH3
-
Clostridium tetanomorphum
r
4.3.1.2
L-threo-3-methylaspartate
Clostridium tetanomorphum
-
?
-
-
-
4.3.1.2
L-threo-3-methylaspartate
Clostridium tetanomorphum H1 / ATCC 15920
-
mesaconate + NH3
-
Clostridium tetanomorphum H1 / ATCC 15920
r
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
4.3.1.2
-
Clostridium tetanomorphum
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4.3.1.2
(2S)-aspartate
-
6064
Clostridium tetanomorphum
fumarate + NH3
-
6064
Clostridium tetanomorphum
?
4.3.1.2
erythro-3-methylaspartate
(2S,3R)-3-methylaspartic acid
6064
Clostridium tetanomorphum
?
-
-
-
-
4.3.1.2
L-aspartate
(2S)-aspartate
6064
Clostridium tetanomorphum
fumarate + NH3
-
-
-
-
4.3.1.2
L-aspartate
(2S)-aspartate
6064
Clostridium tetanomorphum H1 / ATCC 15920
fumarate + NH3
-
-
-
-
4.3.1.2
L-erythro-3-methylaspartate
i.e. (2S,3R)-3-methylaspartate, slow syn elimination
6064
Clostridium tetanomorphum
mesaconate + NH3
-
6064
Clostridium tetanomorphum
?
4.3.1.2
L-threo-3-methylaspartate
-
6064
Clostridium tetanomorphum
mesaconate + NH3
-
6064
Clostridium tetanomorphum
r
4.3.1.2
L-threo-3-methylaspartate
r
6064
Clostridium tetanomorphum
mesaconate + NH3
-
6064
Clostridium tetanomorphum
-
4.3.1.2
L-threo-3-methylaspartate
(2S,3S)-3-methylaspartic acid
6064
Clostridium tetanomorphum
mesaconate + NH3
-
6064
Clostridium tetanomorphum
-
4.3.1.2
L-threo-3-methylaspartate
i.e. (2S,3S)-3-methylaspartate
6064
Clostridium tetanomorphum
mesaconate + NH3
-
6064
Clostridium tetanomorphum
r
4.3.1.2
L-threo-3-methylaspartate
-
6064
Clostridium tetanomorphum H1 / ATCC 15920
mesaconate + NH3
-
6064
Clostridium tetanomorphum H1 / ATCC 15920
r
4.3.1.2
L-threo-3-methylaspartate
r
6064
Clostridium tetanomorphum H1 / ATCC 15920
mesaconate + NH3
-
6064
Clostridium tetanomorphum H1 / ATCC 15920
-
4.3.1.2
L-threo-3-methylaspartate
(2S,3S)-3-methylaspartic acid
6064
Clostridium tetanomorphum H1 / ATCC 15920
mesaconate + NH3
-
6064
Clostridium tetanomorphum H1 / ATCC 15920
-
4.3.1.2
L-threo-3-methylaspartate
i.e. (2S,3S)-3-methylaspartate
6064
Clostridium tetanomorphum H1 / ATCC 15920
mesaconate + NH3
-
6064
Clostridium tetanomorphum H1 / ATCC 15920
r
4.3.1.2
L-threo-3-methylaspartate
-
6064
Clostridium tetanomorphum
?
-
-
-
-