BRENDA - Enzyme Database

Cloning, nucleotide sequencing, and expression of the 3-methylaspartate ammonia-lyase gene from Citrobacter amalonaticus strain YG-1002

Kato, Y.; Asano, Y.; Appl. Microbiol. Biotechnol. 50, 468-474 (1998)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
4.3.1.2
expression in Escherichia coli
Citrobacter amalonaticus
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
4.3.1.2
L-threo-3-methylaspartate
Clostridium tetanomorphum
-
?
-
-
-
4.3.1.2
L-threo-3-methylaspartate
Citrobacter amalonaticus
-
?
-
-
-
4.3.1.2
L-threo-3-methylaspartate
Citrobacter amalonaticus YG-1002
-
?
-
-
-
4.3.1.2
L-threo-3-methylaspartate
Citrobacter amalonaticus TPU 6323
-
?
-
-
-
4.3.1.2
L-threo-3-methylaspartate
Clostridium tetanomorphum NCIMB 11547
-
?
-
-
-
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
4.3.1.2
Citrobacter amalonaticus
-
TPU 6323; YG-1002
-
4.3.1.2
Citrobacter amalonaticus TPU 6323
-
TPU 6323
-
4.3.1.2
Citrobacter amalonaticus YG-1002
-
YG-1002
-
4.3.1.2
Clostridium tetanomorphum
-
NCIMB 11547
-
4.3.1.2
Clostridium tetanomorphum NCIMB 11547
-
NCIMB 11547
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
4.3.1.2
additional information
-
-
Citrobacter amalonaticus
4.3.1.2
additional information
-
-
Clostridium tetanomorphum
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4.3.1.2
L-threo-3-methylaspartate
r
6062
Clostridium tetanomorphum
mesaconate + NH3
-
6062
Clostridium tetanomorphum
-
4.3.1.2
L-threo-3-methylaspartate
r
6062
Citrobacter amalonaticus
mesaconate + NH3
-
6062
Citrobacter amalonaticus
-
4.3.1.2
L-threo-3-methylaspartate
r
6062
Citrobacter amalonaticus YG-1002
mesaconate + NH3
-
6062
Citrobacter amalonaticus YG-1002
-
4.3.1.2
L-threo-3-methylaspartate
r
6062
Citrobacter amalonaticus TPU 6323
mesaconate + NH3
-
6062
Citrobacter amalonaticus TPU 6323
-
4.3.1.2
L-threo-3-methylaspartate
r
6062
Clostridium tetanomorphum NCIMB 11547
mesaconate + NH3
-
6062
Clostridium tetanomorphum NCIMB 11547
-
4.3.1.2
L-threo-3-methylaspartate
-
6062
Clostridium tetanomorphum
?
-
-
-
-
4.3.1.2
L-threo-3-methylaspartate
-
6062
Citrobacter amalonaticus
?
-
-
-
-
4.3.1.2
L-threo-3-methylaspartate
-
6062
Citrobacter amalonaticus YG-1002
?
-
-
-
-
4.3.1.2
L-threo-3-methylaspartate
-
6062
Citrobacter amalonaticus TPU 6323
?
-
-
-
-
4.3.1.2
L-threo-3-methylaspartate
-
6062
Clostridium tetanomorphum NCIMB 11547
?
-
-
-
-
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
4.3.1.2
expression in Escherichia coli
Citrobacter amalonaticus
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
4.3.1.2
L-threo-3-methylaspartate
Clostridium tetanomorphum
-
?
-
-
-
4.3.1.2
L-threo-3-methylaspartate
Citrobacter amalonaticus
-
?
-
-
-
4.3.1.2
L-threo-3-methylaspartate
Citrobacter amalonaticus YG-1002
-
?
-
-
-
4.3.1.2
L-threo-3-methylaspartate
Citrobacter amalonaticus TPU 6323
-
?
-
-
-
4.3.1.2
L-threo-3-methylaspartate
Clostridium tetanomorphum NCIMB 11547
-
?
-
-
-
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
4.3.1.2
additional information
-
-
Citrobacter amalonaticus
4.3.1.2
additional information
-
-
Clostridium tetanomorphum
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4.3.1.2
L-threo-3-methylaspartate
r
6062
Clostridium tetanomorphum
mesaconate + NH3
-
6062
Clostridium tetanomorphum
-
4.3.1.2
L-threo-3-methylaspartate
r
6062
Citrobacter amalonaticus
mesaconate + NH3
-
6062
Citrobacter amalonaticus
-
4.3.1.2
L-threo-3-methylaspartate
r
6062
Citrobacter amalonaticus YG-1002
mesaconate + NH3
-
6062
Citrobacter amalonaticus YG-1002
-
4.3.1.2
L-threo-3-methylaspartate
r
6062
Citrobacter amalonaticus TPU 6323
mesaconate + NH3
-
6062
Citrobacter amalonaticus TPU 6323
-
4.3.1.2
L-threo-3-methylaspartate
r
6062
Clostridium tetanomorphum NCIMB 11547
mesaconate + NH3
-
6062
Clostridium tetanomorphum NCIMB 11547
-
4.3.1.2
L-threo-3-methylaspartate
-
6062
Clostridium tetanomorphum
?
-
-
-
-
4.3.1.2
L-threo-3-methylaspartate
-
6062
Citrobacter amalonaticus
?
-
-
-
-
4.3.1.2
L-threo-3-methylaspartate
-
6062
Citrobacter amalonaticus YG-1002
?
-
-
-
-
4.3.1.2
L-threo-3-methylaspartate
-
6062
Citrobacter amalonaticus TPU 6323
?
-
-
-
-
4.3.1.2
L-threo-3-methylaspartate
-
6062
Clostridium tetanomorphum NCIMB 11547
?
-
-
-
-