BRENDA - Enzyme Database

Purification and properties of crystalline 3-methylaspartase from two facultative anaerobes, Citrobacter sp. strain YG-0504 and Morganella morganii strain YG-0601

Kato, Y.; Asano, Y.; Biosci. Biotechnol. Biochem. 59, 93-99 (1995)

Data extracted from this reference:

Crystallization (Commentary)
EC Number
Crystallization
Organism
4.3.1.2
-
Citrobacter freundii
4.3.1.2
-
Morganella morganii
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
4.3.1.2
5,5'-dithiobis(2-nitrobenzoate)
1 mM, complete
Citrobacter freundii
4.3.1.2
5,5'-dithiobis(2-nitrobenzoate)
1 mM 54%, loss of activity
Morganella morganii
4.3.1.2
8-hydroxyquinoline
5 mM, 44% loss of activity
Citrobacter freundii
4.3.1.2
8-hydroxyquinoline
5 mM, 88% loss of activity
Morganella morganii
4.3.1.2
Ag+
complete
Citrobacter freundii
4.3.1.2
Ag+
complete
Morganella morganii
4.3.1.2
Al3+
5 mM AlCl3, 95% loss of activity
Morganella morganii
4.3.1.2
Ca2+
CaCl2 5 mM, 83% loss of activity
Citrobacter freundii
4.3.1.2
Ca2+
-
Clostridium tetanomorphum
4.3.1.2
Ca2+
5 mM CaCl2, 73% loss of activity
Morganella morganii
4.3.1.2
Cd2+
1 mM CdCl2, complete inhibition
Morganella morganii
4.3.1.2
Co2+
1 mM CoCl2, 60% loss of activity
Morganella morganii
4.3.1.2
Cu2+
1 mM CuSO4, 47% loss of activity
Citrobacter freundii
4.3.1.2
Cu2+
-
Clostridium tetanomorphum
4.3.1.2
Cu2+
1 mM CuSO4, 73% loss of activity
Morganella morganii
4.3.1.2
Fe2+
5 mM FeSO4, 56% loss of activity
Citrobacter freundii
4.3.1.2
Fe2+
5 mM FeSO4, 89% loss of activity
Morganella morganii
4.3.1.2
Fe3+
1 mM, 75% loss of activity
Citrobacter freundii
4.3.1.2
Fe3+
1 mM, 69% loss of activity
Morganella morganii
4.3.1.2
Hg+
complete
Citrobacter freundii
4.3.1.2
Hg+
complete
Morganella morganii
4.3.1.2
Hg2+
complete
Citrobacter freundii
4.3.1.2
Hg2+
complete
Morganella morganii
4.3.1.2
iodoacetate
1 mM, 86% loss of activity
Morganella morganii
4.3.1.2
additional information
-
Citrobacter freundii
4.3.1.2
additional information
-
Morganella morganii
4.3.1.2
N-ethylmaleimide
complete
Citrobacter freundii
4.3.1.2
N-ethylmaleimide
-
Clostridium tetanomorphum
4.3.1.2
N-ethylmaleimide
complete
Morganella morganii
4.3.1.2
p-chloromercuribenzoate
complete
Citrobacter freundii
4.3.1.2
p-chloromercuribenzoate
complete
Morganella morganii
4.3.1.2
Pb2+
5 mM PbCl2, 88% loss of activity
Morganella morganii
4.3.1.2
Sn2+
1 mM SnCl2, 51% loss of activity
Morganella morganii
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
4.3.1.2
Cs+
-
Citrobacter freundii
4.3.1.2
Cs+
-
Morganella morganii
4.3.1.2
Li+
-
Citrobacter freundii
4.3.1.2
Li+
-
Morganella morganii
4.3.1.2
Mg2+
-
Citrobacter freundii
4.3.1.2
Mg2+
-
Morganella morganii
4.3.1.2
Mg2+
-
Clostridium tetanomorphum
4.3.1.2
Mn2+
activates
Citrobacter freundii
4.3.1.2
Mn2+
activates
Clostridium tetanomorphum
4.3.1.2
Mn2+
activates
Morganella morganii
4.3.1.2
Na+
-
Citrobacter freundii
4.3.1.2
Na+
-
Morganella morganii
4.3.1.2
Ni2+
activates
Citrobacter freundii
4.3.1.2
Ni2+
activates
Clostridium tetanomorphum
4.3.1.2
Ni2+
activates
Morganella morganii
4.3.1.2
Rb+
-
Citrobacter freundii
4.3.1.2
Rb+
-
Morganella morganii
4.3.1.2
Zn2+
activates
Citrobacter freundii
4.3.1.2
Zn2+
activates
Clostridium tetanomorphum
4.3.1.2
Zn2+
activates
Morganella morganii
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
4.3.1.2
40000
-
2 * 40000, SDS-PAGE
Citrobacter freundii
4.3.1.2
44000
-
2 * 44000, SDS-PAGE
Morganella morganii
4.3.1.2
70000
-
gel filtration
Morganella morganii
4.3.1.2
79000
-
gel filtration
Citrobacter freundii
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
4.3.1.2
L-threo-3-methylaspartate
Citrobacter freundii
-
?
-
-
-
4.3.1.2
L-threo-3-methylaspartate
Clostridium tetanomorphum
-
?
-
-
-
4.3.1.2
L-threo-3-methylaspartate
Morganella morganii
-
?
-
-
-
4.3.1.2
additional information
Clostridium tetanomorphum
enzyme is associated with an anaerobic degradation of the (S)-glutamic acid in obligat anaerobes
?
-
-
-
4.3.1.2
additional information
Clostridium tetanomorphum NCIMB 11547
enzyme is associated with an anaerobic degradation of the (S)-glutamic acid in obligat anaerobes
?
-
-
-
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
4.3.1.2
Citrobacter freundii
-
Citrobacter sp. strain YG-0504
-
4.3.1.2
Clostridium tetanomorphum
-
NCIMB 11547
-
4.3.1.2
Clostridium tetanomorphum NCIMB 11547
-
NCIMB 11547
-
4.3.1.2
Morganella morganii
-
-
-
4.3.1.2
Morganella morganii YG-0601
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
4.3.1.2
-
Citrobacter freundii
4.3.1.2
-
Morganella morganii
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
4.3.1.2
additional information
-
-
Citrobacter freundii
4.3.1.2
additional information
-
-
Clostridium tetanomorphum
4.3.1.2
additional information
-
-
Morganella morganii
4.3.1.2
162
-
-
Citrobacter freundii
4.3.1.2
296
-
-
Morganella morganii
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4.3.1.2
(2S,3S)-3-ethylaspartic acid
-
6060
Citrobacter freundii
?
-
-
-
-
4.3.1.2
(2S,3S)-3-ethylaspartic acid
-
6060
Clostridium tetanomorphum
?
-
-
-
-
4.3.1.2
(2S,3S)-3-ethylaspartic acid
-
6060
Morganella morganii
?
-
-
-
-
4.3.1.2
3-chloroaspartate
-
6060
Clostridium tetanomorphum
chlorofumarate + NH3
-
6060
Clostridium tetanomorphum
-
4.3.1.2
3-chloroaspartate
(2R,3S)-3-chloroaspartic acid
6060
Citrobacter freundii
chlorofumarate + NH3
-
6060
Citrobacter freundii
-
4.3.1.2
3-chloroaspartate
(2R,3S)-3-chloroaspartic acid
6060
Morganella morganii
chlorofumarate + NH3
-
6060
Morganella morganii
-
4.3.1.2
3-chloroaspartate
-
6060
Clostridium tetanomorphum NCIMB 11547
chlorofumarate + NH3
-
6060
Clostridium tetanomorphum NCIMB 11547
-
4.3.1.2
3-chloroaspartate
(2R,3S)-3-chloroaspartic acid
6060
Morganella morganii YG-0601
chlorofumarate + NH3
-
6060
Morganella morganii YG-0601
-
4.3.1.2
? + NH4Cl
-
6060
Citrobacter freundii
?
-
-
-
-
4.3.1.2
? + NH4Cl
-
6060
Morganella morganii
?
-
-
-
-
4.3.1.2
ethylfumarate + NH3
-
6060
Citrobacter freundii
?
-
-
-
-
4.3.1.2
ethylfumarate + NH3
-
6060
Clostridium tetanomorphum
?
-
-
-
-
4.3.1.2
ethylfumarate + NH3
-
6060
Morganella morganii
?
-
-
-
-
4.3.1.2
ethylfumarate + NH3
-
6060
Morganella morganii YG-0601
?
-
-
-
-
4.3.1.2
L-threo-3-methylaspartate
r
6060
Citrobacter freundii
mesaconate + NH3
-
6060
Citrobacter freundii
-
4.3.1.2
L-threo-3-methylaspartate
r
6060
Clostridium tetanomorphum
mesaconate + NH3
-
6060
Clostridium tetanomorphum
-
4.3.1.2
L-threo-3-methylaspartate
r
6060
Morganella morganii
mesaconate + NH3
-
6060
Morganella morganii
-
4.3.1.2
L-threo-3-methylaspartate
(2S,3S)-3-methylaspartic acid
6060
Citrobacter freundii
mesaconate + NH3
-
6060
Citrobacter freundii
-
4.3.1.2
L-threo-3-methylaspartate
(2S,3S)-3-methylaspartic acid
6060
Clostridium tetanomorphum
mesaconate + NH3
-
6060
Clostridium tetanomorphum
-
4.3.1.2
L-threo-3-methylaspartate
(2S,3S)-3-methylaspartic acid
6060
Morganella morganii
mesaconate + NH3
-
6060
Morganella morganii
-
4.3.1.2
L-threo-3-methylaspartate
r
6060
Clostridium tetanomorphum NCIMB 11547
mesaconate + NH3
-
6060
Clostridium tetanomorphum NCIMB 11547
-
4.3.1.2
L-threo-3-methylaspartate
(2S,3S)-3-methylaspartic acid
6060
Clostridium tetanomorphum NCIMB 11547
mesaconate + NH3
-
6060
Clostridium tetanomorphum NCIMB 11547
-
4.3.1.2
L-threo-3-methylaspartate
r
6060
Morganella morganii YG-0601
mesaconate + NH3
-
6060
Morganella morganii YG-0601
-
4.3.1.2
L-threo-3-methylaspartate
(2S,3S)-3-methylaspartic acid
6060
Morganella morganii YG-0601
mesaconate + NH3
-
6060
Morganella morganii YG-0601
-
4.3.1.2
L-threo-3-methylaspartate
-
6060
Citrobacter freundii
?
-
-
-
-
4.3.1.2
L-threo-3-methylaspartate
-
6060
Clostridium tetanomorphum
?
-
-
-
-
4.3.1.2
L-threo-3-methylaspartate
-
6060
Morganella morganii
?
-
-
-
-
4.3.1.2
additional information
-
6060
Citrobacter freundii
?
-
-
-
-
4.3.1.2
additional information
-
6060
Clostridium tetanomorphum
?
-
-
-
-
4.3.1.2
additional information
-
6060
Morganella morganii
?
-
-
-
-
4.3.1.2
additional information
enzyme is associated with an anaerobic degradation of the (S)-glutamic acid in obligat anaerobes
6060
Clostridium tetanomorphum
?
-
-
-
-
4.3.1.2
additional information
-
6060
Clostridium tetanomorphum NCIMB 11547
?
-
-
-
-
4.3.1.2
additional information
enzyme is associated with an anaerobic degradation of the (S)-glutamic acid in obligat anaerobes
6060
Clostridium tetanomorphum NCIMB 11547
?
-
-
-
-
4.3.1.2
additional information
-
6060
Morganella morganii YG-0601
?
-
-
-
-
Subunits
EC Number
Subunits
Commentary
Organism
4.3.1.2
dimer
2 * 40000, SDS-PAGE
Citrobacter freundii
4.3.1.2
dimer
-
Clostridium tetanomorphum
4.3.1.2
dimer
2 * 44000, SDS-PAGE
Morganella morganii
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
4.3.1.2
45
-
maximal activity in the deamination reaction of L-threo-3-methylaspartate
Morganella morganii
4.3.1.2
50
-
maximal activity in the deamination reaction of L-threo-3-methylaspartate
Citrobacter freundii
4.3.1.2
55
-
-
Clostridium tetanomorphum
Temperature Stability [°C]
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
4.3.1.2
additional information
-
-
Citrobacter freundii
4.3.1.2
additional information
-
-
Morganella morganii
4.3.1.2
45
-
pH 7.0, 10 min, 0.1 M potassium phosphate buffer, deamination reaction of (2S,3S)-3-methylaspartate, 5% loss of activity
Citrobacter freundii
4.3.1.2
45
-
pH 7.0, 10 min, 0.1 M potassium phosphate buffer, deamination reaction of (2S,3S)-3-methylaspartate, 5% loss of activity
Morganella morganii
4.3.1.2
50
-
0.1 mM potassium phosphate buffer, pH 7.0, 10 min, in the deamination reaction of (2S,3S)-3-methylaspartate, 50% loss of activity
Citrobacter freundii
4.3.1.2
50
-
0.1 mM potassium phosphate buffer, pH 7.0, 10 min, in the deamination reaction of (2S,3S)-3-methylaspartate, 50% loss of activity
Morganella morganii
4.3.1.2
55
-
0.1 mM potassium phosphate buffer, pH 7.0, 10 min, in the deamination reaction of (2S,3S)-3-methylaspartate, most of activity is lost
Citrobacter freundii
4.3.1.2
55
-
0.1 mM potassium phosphate buffer, pH 7.0, 10 min, in the deamination reaction of (2S,3S)-3-methylaspartate, most of activity is lost
Morganella morganii
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
4.3.1.2
8
-
maximal activity in TRIS/HCl buffer in the amination reaction of mesaconate
Morganella morganii
4.3.1.2
8.5
-
maximal activity in TRIS/HCl buffer in the amination reaction of mesaconate
Citrobacter freundii
4.3.1.2
9.7
-
maximal activity in ethanolamine/HCl buffer in the deamination reaction of L-threo-3-methylaspartate
Citrobacter freundii
4.3.1.2
9.7
-
maximal activity in ethanolamine/HCl buffer in the deamination reaction of L-threo-3-methylaspartate
Morganella morganii
pH Range
EC Number
pH Minimum
pH Maximum
Commentary
Organism
4.3.1.2
6
11
pH 6.0: 10% of maximal activity, pH 11.0: 40% of maximal activity in the amination reaction of mesaconate
Morganella morganii
4.3.1.2
7
11
pH 7.0: about 10% of maximal activity, pH 11.0: about 90% of maximal activity in the amination reaction of mesaconate; pH 7.0: about 25% of maximal activity, pH 11.0: about 5% of maximal activity, in the deamination reaction of L-threo-3-methylaspartate
Citrobacter freundii
4.3.1.2
7
11
pH 7.0: about 10% of maximal activity, pH 11.0: about 80% of maximal activity in the deamination reaction of L-threo-3-methylaspartate
Morganella morganii
pH Stability
EC Number
pH Stability
pH Stability Maximum
Commentary
Organism
4.3.1.2
additional information
-
-
Citrobacter freundii
4.3.1.2
additional information
-
-
Morganella morganii
4.3.1.2
5
10
stable between, pH 5.0: inactivated rapidly below
Citrobacter freundii
4.3.1.2
6
8.5
stable between, pH 5.0: inactivated rapidly below
Morganella morganii
Crystallization (Commentary) (protein specific)
EC Number
Crystallization
Organism
4.3.1.2
-
Citrobacter freundii
4.3.1.2
-
Morganella morganii
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
4.3.1.2
5,5'-dithiobis(2-nitrobenzoate)
1 mM, complete
Citrobacter freundii
4.3.1.2
5,5'-dithiobis(2-nitrobenzoate)
1 mM 54%, loss of activity
Morganella morganii
4.3.1.2
8-hydroxyquinoline
5 mM, 44% loss of activity
Citrobacter freundii
4.3.1.2
8-hydroxyquinoline
5 mM, 88% loss of activity
Morganella morganii
4.3.1.2
Ag+
complete
Citrobacter freundii
4.3.1.2
Ag+
complete
Morganella morganii
4.3.1.2
Al3+
5 mM AlCl3, 95% loss of activity
Morganella morganii
4.3.1.2
Ca2+
CaCl2 5 mM, 83% loss of activity
Citrobacter freundii
4.3.1.2
Ca2+
-
Clostridium tetanomorphum
4.3.1.2
Ca2+
5 mM CaCl2, 73% loss of activity
Morganella morganii
4.3.1.2
Cd2+
1 mM CdCl2, complete inhibition
Morganella morganii
4.3.1.2
Co2+
1 mM CoCl2, 60% loss of activity
Morganella morganii
4.3.1.2
Cu2+
1 mM CuSO4, 47% loss of activity
Citrobacter freundii
4.3.1.2
Cu2+
-
Clostridium tetanomorphum
4.3.1.2
Cu2+
1 mM CuSO4, 73% loss of activity
Morganella morganii
4.3.1.2
Fe2+
5 mM FeSO4, 56% loss of activity
Citrobacter freundii
4.3.1.2
Fe2+
5 mM FeSO4, 89% loss of activity
Morganella morganii
4.3.1.2
Fe3+
1 mM, 75% loss of activity
Citrobacter freundii
4.3.1.2
Fe3+
1 mM, 69% loss of activity
Morganella morganii
4.3.1.2
Hg+
complete
Citrobacter freundii
4.3.1.2
Hg+
complete
Morganella morganii
4.3.1.2
Hg2+
complete
Citrobacter freundii
4.3.1.2
Hg2+
complete
Morganella morganii
4.3.1.2
iodoacetate
1 mM, 86% loss of activity
Morganella morganii
4.3.1.2
additional information
-
Citrobacter freundii
4.3.1.2
additional information
-
Morganella morganii
4.3.1.2
N-ethylmaleimide
complete
Citrobacter freundii
4.3.1.2
N-ethylmaleimide
-
Clostridium tetanomorphum
4.3.1.2
N-ethylmaleimide
complete
Morganella morganii
4.3.1.2
p-chloromercuribenzoate
complete
Citrobacter freundii
4.3.1.2
p-chloromercuribenzoate
complete
Morganella morganii
4.3.1.2
Pb2+
5 mM PbCl2, 88% loss of activity
Morganella morganii
4.3.1.2
Sn2+
1 mM SnCl2, 51% loss of activity
Morganella morganii
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
4.3.1.2
Cs+
-
Citrobacter freundii
4.3.1.2
Cs+
-
Morganella morganii
4.3.1.2
Li+
-
Citrobacter freundii
4.3.1.2
Li+
-
Morganella morganii
4.3.1.2
Mg2+
-
Citrobacter freundii
4.3.1.2
Mg2+
-
Morganella morganii
4.3.1.2
Mg2+
-
Clostridium tetanomorphum
4.3.1.2
Mn2+
activates
Citrobacter freundii
4.3.1.2
Mn2+
activates
Clostridium tetanomorphum
4.3.1.2
Mn2+
activates
Morganella morganii
4.3.1.2
Na+
-
Citrobacter freundii
4.3.1.2
Na+
-
Morganella morganii
4.3.1.2
Ni2+
activates
Citrobacter freundii
4.3.1.2
Ni2+
activates
Clostridium tetanomorphum
4.3.1.2
Ni2+
activates
Morganella morganii
4.3.1.2
Rb+
-
Citrobacter freundii
4.3.1.2
Rb+
-
Morganella morganii
4.3.1.2
Zn2+
activates
Citrobacter freundii
4.3.1.2
Zn2+
activates
Clostridium tetanomorphum
4.3.1.2
Zn2+
activates
Morganella morganii
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
4.3.1.2
40000
-
2 * 40000, SDS-PAGE
Citrobacter freundii
4.3.1.2
44000
-
2 * 44000, SDS-PAGE
Morganella morganii
4.3.1.2
70000
-
gel filtration
Morganella morganii
4.3.1.2
79000
-
gel filtration
Citrobacter freundii
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
4.3.1.2
L-threo-3-methylaspartate
Citrobacter freundii
-
?
-
-
-
4.3.1.2
L-threo-3-methylaspartate
Clostridium tetanomorphum
-
?
-
-
-
4.3.1.2
L-threo-3-methylaspartate
Morganella morganii
-
?
-
-
-
4.3.1.2
additional information
Clostridium tetanomorphum
enzyme is associated with an anaerobic degradation of the (S)-glutamic acid in obligat anaerobes
?
-
-
-
4.3.1.2
additional information
Clostridium tetanomorphum NCIMB 11547
enzyme is associated with an anaerobic degradation of the (S)-glutamic acid in obligat anaerobes
?
-
-
-
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
4.3.1.2
-
Citrobacter freundii
4.3.1.2
-
Morganella morganii
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
4.3.1.2
additional information
-
-
Citrobacter freundii
4.3.1.2
additional information
-
-
Clostridium tetanomorphum
4.3.1.2
additional information
-
-
Morganella morganii
4.3.1.2
162
-
-
Citrobacter freundii
4.3.1.2
296
-
-
Morganella morganii
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4.3.1.2
(2S,3S)-3-ethylaspartic acid
-
6060
Citrobacter freundii
?
-
-
-
-
4.3.1.2
(2S,3S)-3-ethylaspartic acid
-
6060
Clostridium tetanomorphum
?
-
-
-
-
4.3.1.2
(2S,3S)-3-ethylaspartic acid
-
6060
Morganella morganii
?
-
-
-
-
4.3.1.2
3-chloroaspartate
-
6060
Clostridium tetanomorphum
chlorofumarate + NH3
-
6060
Clostridium tetanomorphum
-
4.3.1.2
3-chloroaspartate
(2R,3S)-3-chloroaspartic acid
6060
Citrobacter freundii
chlorofumarate + NH3
-
6060
Citrobacter freundii
-
4.3.1.2
3-chloroaspartate
(2R,3S)-3-chloroaspartic acid
6060
Morganella morganii
chlorofumarate + NH3
-
6060
Morganella morganii
-
4.3.1.2
3-chloroaspartate
-
6060
Clostridium tetanomorphum NCIMB 11547
chlorofumarate + NH3
-
6060
Clostridium tetanomorphum NCIMB 11547
-
4.3.1.2
3-chloroaspartate
(2R,3S)-3-chloroaspartic acid
6060
Morganella morganii YG-0601
chlorofumarate + NH3
-
6060
Morganella morganii YG-0601
-
4.3.1.2
? + NH4Cl
-
6060
Citrobacter freundii
?
-
-
-
-
4.3.1.2
? + NH4Cl
-
6060
Morganella morganii
?
-
-
-
-
4.3.1.2
ethylfumarate + NH3
-
6060
Citrobacter freundii
?
-
-
-
-
4.3.1.2
ethylfumarate + NH3
-
6060
Clostridium tetanomorphum
?
-
-
-
-
4.3.1.2
ethylfumarate + NH3
-
6060
Morganella morganii
?
-
-
-
-
4.3.1.2
ethylfumarate + NH3
-
6060
Morganella morganii YG-0601
?
-
-
-
-
4.3.1.2
L-threo-3-methylaspartate
r
6060
Citrobacter freundii
mesaconate + NH3
-
6060
Citrobacter freundii
-
4.3.1.2
L-threo-3-methylaspartate
r
6060
Clostridium tetanomorphum
mesaconate + NH3
-
6060
Clostridium tetanomorphum
-
4.3.1.2
L-threo-3-methylaspartate
r
6060
Morganella morganii
mesaconate + NH3
-
6060
Morganella morganii
-
4.3.1.2
L-threo-3-methylaspartate
(2S,3S)-3-methylaspartic acid
6060
Citrobacter freundii
mesaconate + NH3
-
6060
Citrobacter freundii
-
4.3.1.2
L-threo-3-methylaspartate
(2S,3S)-3-methylaspartic acid
6060
Clostridium tetanomorphum
mesaconate + NH3
-
6060
Clostridium tetanomorphum
-
4.3.1.2
L-threo-3-methylaspartate
(2S,3S)-3-methylaspartic acid
6060
Morganella morganii
mesaconate + NH3
-
6060
Morganella morganii
-
4.3.1.2
L-threo-3-methylaspartate
r
6060
Clostridium tetanomorphum NCIMB 11547
mesaconate + NH3
-
6060
Clostridium tetanomorphum NCIMB 11547
-
4.3.1.2
L-threo-3-methylaspartate
(2S,3S)-3-methylaspartic acid
6060
Clostridium tetanomorphum NCIMB 11547
mesaconate + NH3
-
6060
Clostridium tetanomorphum NCIMB 11547
-
4.3.1.2
L-threo-3-methylaspartate
r
6060
Morganella morganii YG-0601
mesaconate + NH3
-
6060
Morganella morganii YG-0601
-
4.3.1.2
L-threo-3-methylaspartate
(2S,3S)-3-methylaspartic acid
6060
Morganella morganii YG-0601
mesaconate + NH3
-
6060
Morganella morganii YG-0601
-
4.3.1.2
L-threo-3-methylaspartate
-
6060
Citrobacter freundii
?
-
-
-
-
4.3.1.2
L-threo-3-methylaspartate
-
6060
Clostridium tetanomorphum
?
-
-
-
-
4.3.1.2
L-threo-3-methylaspartate
-
6060
Morganella morganii
?
-
-
-
-
4.3.1.2
additional information
-
6060
Citrobacter freundii
?
-
-
-
-
4.3.1.2
additional information
-
6060
Clostridium tetanomorphum
?
-
-
-
-
4.3.1.2
additional information
-
6060
Morganella morganii
?
-
-
-
-
4.3.1.2
additional information
enzyme is associated with an anaerobic degradation of the (S)-glutamic acid in obligat anaerobes
6060
Clostridium tetanomorphum
?
-
-
-
-
4.3.1.2
additional information
-
6060
Clostridium tetanomorphum NCIMB 11547
?
-
-
-
-
4.3.1.2
additional information
enzyme is associated with an anaerobic degradation of the (S)-glutamic acid in obligat anaerobes
6060
Clostridium tetanomorphum NCIMB 11547
?
-
-
-
-
4.3.1.2
additional information
-
6060
Morganella morganii YG-0601
?
-
-
-
-
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
4.3.1.2
dimer
2 * 40000, SDS-PAGE
Citrobacter freundii
4.3.1.2
dimer
-
Clostridium tetanomorphum
4.3.1.2
dimer
2 * 44000, SDS-PAGE
Morganella morganii
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
4.3.1.2
45
-
maximal activity in the deamination reaction of L-threo-3-methylaspartate
Morganella morganii
4.3.1.2
50
-
maximal activity in the deamination reaction of L-threo-3-methylaspartate
Citrobacter freundii
4.3.1.2
55
-
-
Clostridium tetanomorphum
Temperature Stability [°C] (protein specific)
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
4.3.1.2
additional information
-
-
Citrobacter freundii
4.3.1.2
additional information
-
-
Morganella morganii
4.3.1.2
45
-
pH 7.0, 10 min, 0.1 M potassium phosphate buffer, deamination reaction of (2S,3S)-3-methylaspartate, 5% loss of activity
Citrobacter freundii
4.3.1.2
45
-
pH 7.0, 10 min, 0.1 M potassium phosphate buffer, deamination reaction of (2S,3S)-3-methylaspartate, 5% loss of activity
Morganella morganii
4.3.1.2
50
-
0.1 mM potassium phosphate buffer, pH 7.0, 10 min, in the deamination reaction of (2S,3S)-3-methylaspartate, 50% loss of activity
Citrobacter freundii
4.3.1.2
50
-
0.1 mM potassium phosphate buffer, pH 7.0, 10 min, in the deamination reaction of (2S,3S)-3-methylaspartate, 50% loss of activity
Morganella morganii
4.3.1.2
55
-
0.1 mM potassium phosphate buffer, pH 7.0, 10 min, in the deamination reaction of (2S,3S)-3-methylaspartate, most of activity is lost
Citrobacter freundii
4.3.1.2
55
-
0.1 mM potassium phosphate buffer, pH 7.0, 10 min, in the deamination reaction of (2S,3S)-3-methylaspartate, most of activity is lost
Morganella morganii
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
4.3.1.2
8
-
maximal activity in TRIS/HCl buffer in the amination reaction of mesaconate
Morganella morganii
4.3.1.2
8.5
-
maximal activity in TRIS/HCl buffer in the amination reaction of mesaconate
Citrobacter freundii
4.3.1.2
9.7
-
maximal activity in ethanolamine/HCl buffer in the deamination reaction of L-threo-3-methylaspartate
Citrobacter freundii
4.3.1.2
9.7
-
maximal activity in ethanolamine/HCl buffer in the deamination reaction of L-threo-3-methylaspartate
Morganella morganii
pH Range (protein specific)
EC Number
pH Minimum
pH Maximum
Commentary
Organism
4.3.1.2
6
11
pH 6.0: 10% of maximal activity, pH 11.0: 40% of maximal activity in the amination reaction of mesaconate
Morganella morganii
4.3.1.2
7
11
pH 7.0: about 10% of maximal activity, pH 11.0: about 90% of maximal activity in the amination reaction of mesaconate; pH 7.0: about 25% of maximal activity, pH 11.0: about 5% of maximal activity, in the deamination reaction of L-threo-3-methylaspartate
Citrobacter freundii
4.3.1.2
7
11
pH 7.0: about 10% of maximal activity, pH 11.0: about 80% of maximal activity in the deamination reaction of L-threo-3-methylaspartate
Morganella morganii
pH Stability (protein specific)
EC Number
pH Stability
pH Stability Maximum
Commentary
Organism
4.3.1.2
additional information
-
-
Citrobacter freundii
4.3.1.2
additional information
-
-
Morganella morganii
4.3.1.2
5
10
stable between, pH 5.0: inactivated rapidly below
Citrobacter freundii
4.3.1.2
6
8.5
stable between, pH 5.0: inactivated rapidly below
Morganella morganii