BRENDA - Enzyme Database

3-Methylaspartate ammonia-lyase from a facultative anaerobe, strain YG-1002

Kato, Y.; Asano, Y.; Appl. Microbiol. Biotechnol. 43, 901-907 (1995)

Data extracted from this reference:

Crystallization (Commentary)
EC Number
Crystallization
Organism
4.3.1.2
-
Citrobacter amalonaticus
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
4.3.1.2
2,2'-dipyridyl
70% loss of activity in the deamination reaction of L-threo-3-methylaspartate
Citrobacter amalonaticus
4.3.1.2
5,5'-dithiobis(2-nitrobenzoate)
complete inhibition in the deamination reaction
Citrobacter amalonaticus
4.3.1.2
8-hydroxyquinoline
58% loss of activity in the deamination reaction of L-threo-3-methylaspartate
Citrobacter amalonaticus
4.3.1.2
Ag+
complete
Citrobacter amalonaticus
4.3.1.2
Ca2+
55% loss of activity in the deamination reaction of L-threo-3-methylaspartate
Citrobacter amalonaticus
4.3.1.2
Ca2+
-
Clostridium tetanomorphum
4.3.1.2
Cd2+
complete inhibition in the deamination reaction with L-threo-3-methylaspartate
Citrobacter amalonaticus
4.3.1.2
Co2+
58% loss of activity in the deamination reaction of L-threo-3-methylaspartate
Citrobacter amalonaticus
4.3.1.2
Cu2+
50% loss of activity in the deamination reaction of L-threo-3-methylaspartate
Citrobacter amalonaticus
4.3.1.2
Cu2+
-
Clostridium tetanomorphum
4.3.1.2
Fe2+
43% loss of activity in the deamination reaction of L-threo-3-methylaspartate
Citrobacter amalonaticus
4.3.1.2
Hg+
complete
Citrobacter amalonaticus
4.3.1.2
Hg2+
complete
Citrobacter amalonaticus
4.3.1.2
hydroxylamine
-
Clostridium tetanomorphum
4.3.1.2
additional information
-
Citrobacter amalonaticus
4.3.1.2
N-ethylmaleimide
36% loss of activity in the deamination raction of L-threo-3-methylaspartate
Citrobacter amalonaticus
4.3.1.2
N-ethylmaleimide
-
Clostridium tetanomorphum
4.3.1.2
o-phenanthroline
24% loss of activity in the deamination reaction of L-threo-3-methylaspartate
Citrobacter amalonaticus
4.3.1.2
p-chloromercuribenzoate
complete
Citrobacter amalonaticus
4.3.1.2
phenylhydrazine
-
Clostridium tetanomorphum
4.3.1.2
Sn2+
34% loss of activity in the deamination reaction of L-threo-3-methylaspartate
Citrobacter amalonaticus
4.3.1.2
Zn2+
30% loss of activity in the deamination reaction of L-threo-3-methylaspartate
Citrobacter amalonaticus
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
4.3.1.2
Co2+
activates
Citrobacter amalonaticus
4.3.1.2
Co2+
activates
Clostridium tetanomorphum
4.3.1.2
Cs+
required
Citrobacter amalonaticus
4.3.1.2
K+
-
Citrobacter amalonaticus
4.3.1.2
K+
-
Clostridium tetanomorphum
4.3.1.2
Li+
required
Citrobacter amalonaticus
4.3.1.2
Mg2+
-
Citrobacter amalonaticus
4.3.1.2
Mg2+
-
Clostridium tetanomorphum
4.3.1.2
Mn2+
activates
Citrobacter amalonaticus
4.3.1.2
Mn2+
activates
Clostridium tetanomorphum
4.3.1.2
additional information
-
Citrobacter amalonaticus
4.3.1.2
additional information
-
Clostridium tetanomorphum
4.3.1.2
Na+
required
Citrobacter amalonaticus
4.3.1.2
NH4+
required
Citrobacter amalonaticus
4.3.1.2
Ni2+
activates
Citrobacter amalonaticus
4.3.1.2
Ni2+
activates
Clostridium tetanomorphum
4.3.1.2
Rb+
required
Citrobacter amalonaticus
4.3.1.2
Zn2+
activates
Citrobacter amalonaticus
4.3.1.2
Zn2+
activates
Clostridium tetanomorphum
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
4.3.1.2
42000
-
2 * 42000, SDS-PAGE
Citrobacter amalonaticus
4.3.1.2
84000
-
gel filtration
Citrobacter amalonaticus
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
4.3.1.2
L-threo-3-methylaspartate
Clostridium tetanomorphum
-
?
-
-
-
4.3.1.2
L-threo-3-methylaspartate
Citrobacter amalonaticus
-
?
-
-
-
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
4.3.1.2
Citrobacter amalonaticus
-
YG-1002
-
4.3.1.2
Citrobacter amalonaticus YG-1002
-
YG-1002
-
4.3.1.2
Clostridium tetanomorphum
-
; NCIMB 11547
-
4.3.1.2
Clostridium tetanomorphum H1 / ATCC 15920
-
-
-
4.3.1.2
Clostridium tetanomorphum NCIMB 11547
-
NCIMB 11547
-
Purification (Commentary)
EC Number
Commentary
Organism
4.3.1.2
-
Citrobacter amalonaticus
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
4.3.1.2
additional information
-
-
Citrobacter amalonaticus
4.3.1.2
additional information
-
-
Clostridium tetanomorphum
4.3.1.2
179
-
-
Citrobacter amalonaticus
4.3.1.2
282
-
incubation with 5 mM EDTA, pH 7.0, 4C, for 3 h
Citrobacter amalonaticus
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4.3.1.2
(2S,3R)-3-ethylaspartic acid
-
6059
Clostridium tetanomorphum
?
-
-
-
-
4.3.1.2
(2S,3R)-3-ethylaspartic acid
-
6059
Citrobacter amalonaticus
?
-
-
-
-
4.3.1.2
(2S,3S)-3-ethylaspartic acid
-
6059
Clostridium tetanomorphum
?
-
-
-
-
4.3.1.2
(2S,3S)-3-ethylaspartic acid
-
6059
Citrobacter amalonaticus
?
-
-
-
-
4.3.1.2
3-chloroaspartate
-
6059
Clostridium tetanomorphum
chlorofumarate + NH3
-
6059
Clostridium tetanomorphum
-
4.3.1.2
3-chloroaspartate
(2R,3S)-3-chloroaspartic acid
6059
Citrobacter amalonaticus
chlorofumarate + NH3
-
6059
Citrobacter amalonaticus
-
4.3.1.2
3-chloroaspartate
(2R,3S)-3-chloroaspartic acid
6059
Citrobacter amalonaticus YG-1002
chlorofumarate + NH3
-
6059
Citrobacter amalonaticus YG-1002
-
4.3.1.2
3-chloroaspartate
-
6059
Clostridium tetanomorphum H1 / ATCC 15920
chlorofumarate + NH3
-
6059
Clostridium tetanomorphum H1 / ATCC 15920
-
4.3.1.2
3-chloroaspartate
-
6059
Clostridium tetanomorphum NCIMB 11547
chlorofumarate + NH3
-
6059
Clostridium tetanomorphum NCIMB 11547
-
4.3.1.2
ethylfumarate + NH3
-
6059
Clostridium tetanomorphum
?
-
-
-
-
4.3.1.2
ethylfumarate + NH3
-
6059
Citrobacter amalonaticus
?
-
-
-
-
4.3.1.2
ethylfumarate + NH3
-
6059
Citrobacter amalonaticus YG-1002
?
-
-
-
-
4.3.1.2
ethylfumarate + NH3
-
6059
Clostridium tetanomorphum H1 / ATCC 15920
?
-
-
-
-
4.3.1.2
ethylfumarate + NH3
-
6059
Clostridium tetanomorphum NCIMB 11547
?
-
-
-
-
4.3.1.2
L-threo-3-methylaspartate
r
6059
Clostridium tetanomorphum
mesaconate + NH3
-
6059
Clostridium tetanomorphum
-
4.3.1.2
L-threo-3-methylaspartate
r
6059
Citrobacter amalonaticus
mesaconate + NH3
-
6059
Citrobacter amalonaticus
-
4.3.1.2
L-threo-3-methylaspartate
(2S,3S)-3-methylaspartic acid
6059
Clostridium tetanomorphum
mesaconate + NH3
-
6059
Clostridium tetanomorphum
-
4.3.1.2
L-threo-3-methylaspartate
(2S,3S)-3-methylaspartic acid
6059
Citrobacter amalonaticus
mesaconate + NH3
-
6059
Citrobacter amalonaticus
-
4.3.1.2
L-threo-3-methylaspartate
r
6059
Citrobacter amalonaticus YG-1002
mesaconate + NH3
-
6059
Citrobacter amalonaticus YG-1002
-
4.3.1.2
L-threo-3-methylaspartate
(2S,3S)-3-methylaspartic acid
6059
Citrobacter amalonaticus YG-1002
mesaconate + NH3
-
6059
Citrobacter amalonaticus YG-1002
-
4.3.1.2
L-threo-3-methylaspartate
r
6059
Clostridium tetanomorphum H1 / ATCC 15920
mesaconate + NH3
-
6059
Clostridium tetanomorphum H1 / ATCC 15920
-
4.3.1.2
L-threo-3-methylaspartate
(2S,3S)-3-methylaspartic acid
6059
Clostridium tetanomorphum H1 / ATCC 15920
mesaconate + NH3
-
6059
Clostridium tetanomorphum H1 / ATCC 15920
-
4.3.1.2
L-threo-3-methylaspartate
r
6059
Clostridium tetanomorphum NCIMB 11547
mesaconate + NH3
-
6059
Clostridium tetanomorphum NCIMB 11547
-
4.3.1.2
L-threo-3-methylaspartate
(2S,3S)-3-methylaspartic acid
6059
Clostridium tetanomorphum NCIMB 11547
mesaconate + NH3
-
6059
Clostridium tetanomorphum NCIMB 11547
-
4.3.1.2
L-threo-3-methylaspartate
-
6059
Clostridium tetanomorphum
?
-
-
-
-
4.3.1.2
L-threo-3-methylaspartate
-
6059
Citrobacter amalonaticus
?
-
-
-
-
4.3.1.2
additional information
-
6059
Clostridium tetanomorphum
?
-
-
-
-
4.3.1.2
additional information
-
6059
Citrobacter amalonaticus
?
-
-
-
-
4.3.1.2
additional information
-
6059
Citrobacter amalonaticus YG-1002
?
-
-
-
-
4.3.1.2
additional information
-
6059
Clostridium tetanomorphum H1 / ATCC 15920
?
-
-
-
-
4.3.1.2
additional information
-
6059
Clostridium tetanomorphum NCIMB 11547
?
-
-
-
-
Subunits
EC Number
Subunits
Commentary
Organism
4.3.1.2
dimer
2 * 42000, SDS-PAGE
Citrobacter amalonaticus
4.3.1.2
dimer
-
Clostridium tetanomorphum
Temperature Optimum [C]
EC Number
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
4.3.1.2
50
-
maximal activity in the deamination reaction of L-threo-3-methylaspartate
Citrobacter amalonaticus
Temperature Stability [C]
EC Number
Temperature Stability Minimum [C]
Temperature Stability Maximum [C]
Commentary
Organism
4.3.1.2
45
-
0.1 mM potassium phosphate buffer, pH 7.0, 10 min, in the deamination reaction of (2S,3S)-3-methylaspartate, 6% loss of activity
Citrobacter amalonaticus
4.3.1.2
50
-
0.1 mM potassium phosphate buffer, pH 7.0, 10 min, in the deamination reaction of (2S,3S)-3-methylaspartate, 10% loss of activity
Citrobacter amalonaticus
4.3.1.2
55
-
0.1 mM potassium phosphate buffer, pH 7.0, 10 min, in the deamination reaction of (2S,3S)-3-methylaspartate, 44% loss of activity
Citrobacter amalonaticus
4.3.1.2
60
-
0.1 mM potassium phosphate buffer, pH 7.0, 10 min, in the deamination reaction of (2S,3S)-3-methxylaspartate, 96.5% loss of activity
Citrobacter amalonaticus
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
4.3.1.2
8.5
-
maximal activity in TRIS/HCl buffer in the amination reaction of mesaconate
Citrobacter amalonaticus
4.3.1.2
9.7
-
maximal activity in ethanolamine/HCl buffer in the deamination reaction of L-threo-3-methylaspartate
Citrobacter amalonaticus
pH Stability
EC Number
pH Stability
pH Stability Maximum
Commentary
Organism
4.3.1.2
additional information
-
-
Citrobacter amalonaticus
4.3.1.2
5.5
11
30C, 10 min, deamination reaction of L-threo-3-methylaspartate, 14% loss of activity
Citrobacter amalonaticus
Crystallization (Commentary) (protein specific)
EC Number
Crystallization
Organism
4.3.1.2
-
Citrobacter amalonaticus
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
4.3.1.2
2,2'-dipyridyl
70% loss of activity in the deamination reaction of L-threo-3-methylaspartate
Citrobacter amalonaticus
4.3.1.2
5,5'-dithiobis(2-nitrobenzoate)
complete inhibition in the deamination reaction
Citrobacter amalonaticus
4.3.1.2
8-hydroxyquinoline
58% loss of activity in the deamination reaction of L-threo-3-methylaspartate
Citrobacter amalonaticus
4.3.1.2
Ag+
complete
Citrobacter amalonaticus
4.3.1.2
Ca2+
55% loss of activity in the deamination reaction of L-threo-3-methylaspartate
Citrobacter amalonaticus
4.3.1.2
Ca2+
-
Clostridium tetanomorphum
4.3.1.2
Cd2+
complete inhibition in the deamination reaction with L-threo-3-methylaspartate
Citrobacter amalonaticus
4.3.1.2
Co2+
58% loss of activity in the deamination reaction of L-threo-3-methylaspartate
Citrobacter amalonaticus
4.3.1.2
Cu2+
50% loss of activity in the deamination reaction of L-threo-3-methylaspartate
Citrobacter amalonaticus
4.3.1.2
Cu2+
-
Clostridium tetanomorphum
4.3.1.2
Fe2+
43% loss of activity in the deamination reaction of L-threo-3-methylaspartate
Citrobacter amalonaticus
4.3.1.2
Hg+
complete
Citrobacter amalonaticus
4.3.1.2
Hg2+
complete
Citrobacter amalonaticus
4.3.1.2
hydroxylamine
-
Clostridium tetanomorphum
4.3.1.2
additional information
-
Citrobacter amalonaticus
4.3.1.2
N-ethylmaleimide
36% loss of activity in the deamination raction of L-threo-3-methylaspartate
Citrobacter amalonaticus
4.3.1.2
N-ethylmaleimide
-
Clostridium tetanomorphum
4.3.1.2
o-phenanthroline
24% loss of activity in the deamination reaction of L-threo-3-methylaspartate
Citrobacter amalonaticus
4.3.1.2
p-chloromercuribenzoate
complete
Citrobacter amalonaticus
4.3.1.2
phenylhydrazine
-
Clostridium tetanomorphum
4.3.1.2
Sn2+
34% loss of activity in the deamination reaction of L-threo-3-methylaspartate
Citrobacter amalonaticus
4.3.1.2
Zn2+
30% loss of activity in the deamination reaction of L-threo-3-methylaspartate
Citrobacter amalonaticus
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
4.3.1.2
Co2+
activates
Citrobacter amalonaticus
4.3.1.2
Co2+
activates
Clostridium tetanomorphum
4.3.1.2
Cs+
required
Citrobacter amalonaticus
4.3.1.2
K+
-
Citrobacter amalonaticus
4.3.1.2
K+
-
Clostridium tetanomorphum
4.3.1.2
Li+
required
Citrobacter amalonaticus
4.3.1.2
Mg2+
-
Citrobacter amalonaticus
4.3.1.2
Mg2+
-
Clostridium tetanomorphum
4.3.1.2
Mn2+
activates
Citrobacter amalonaticus
4.3.1.2
Mn2+
activates
Clostridium tetanomorphum
4.3.1.2
additional information
-
Citrobacter amalonaticus
4.3.1.2
additional information
-
Clostridium tetanomorphum
4.3.1.2
Na+
required
Citrobacter amalonaticus
4.3.1.2
NH4+
required
Citrobacter amalonaticus
4.3.1.2
Ni2+
activates
Citrobacter amalonaticus
4.3.1.2
Ni2+
activates
Clostridium tetanomorphum
4.3.1.2
Rb+
required
Citrobacter amalonaticus
4.3.1.2
Zn2+
activates
Citrobacter amalonaticus
4.3.1.2
Zn2+
activates
Clostridium tetanomorphum
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
4.3.1.2
42000
-
2 * 42000, SDS-PAGE
Citrobacter amalonaticus
4.3.1.2
84000
-
gel filtration
Citrobacter amalonaticus
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
4.3.1.2
L-threo-3-methylaspartate
Clostridium tetanomorphum
-
?
-
-
-
4.3.1.2
L-threo-3-methylaspartate
Citrobacter amalonaticus
-
?
-
-
-
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
4.3.1.2
-
Citrobacter amalonaticus
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
4.3.1.2
additional information
-
-
Citrobacter amalonaticus
4.3.1.2
additional information
-
-
Clostridium tetanomorphum
4.3.1.2
179
-
-
Citrobacter amalonaticus
4.3.1.2
282
-
incubation with 5 mM EDTA, pH 7.0, 4C, for 3 h
Citrobacter amalonaticus
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4.3.1.2
(2S,3R)-3-ethylaspartic acid
-
6059
Clostridium tetanomorphum
?
-
-
-
-
4.3.1.2
(2S,3R)-3-ethylaspartic acid
-
6059
Citrobacter amalonaticus
?
-
-
-
-
4.3.1.2
(2S,3S)-3-ethylaspartic acid
-
6059
Clostridium tetanomorphum
?
-
-
-
-
4.3.1.2
(2S,3S)-3-ethylaspartic acid
-
6059
Citrobacter amalonaticus
?
-
-
-
-
4.3.1.2
3-chloroaspartate
-
6059
Clostridium tetanomorphum
chlorofumarate + NH3
-
6059
Clostridium tetanomorphum
-
4.3.1.2
3-chloroaspartate
(2R,3S)-3-chloroaspartic acid
6059
Citrobacter amalonaticus
chlorofumarate + NH3
-
6059
Citrobacter amalonaticus
-
4.3.1.2
3-chloroaspartate
(2R,3S)-3-chloroaspartic acid
6059
Citrobacter amalonaticus YG-1002
chlorofumarate + NH3
-
6059
Citrobacter amalonaticus YG-1002
-
4.3.1.2
3-chloroaspartate
-
6059
Clostridium tetanomorphum H1 / ATCC 15920
chlorofumarate + NH3
-
6059
Clostridium tetanomorphum H1 / ATCC 15920
-
4.3.1.2
3-chloroaspartate
-
6059
Clostridium tetanomorphum NCIMB 11547
chlorofumarate + NH3
-
6059
Clostridium tetanomorphum NCIMB 11547
-
4.3.1.2
ethylfumarate + NH3
-
6059
Clostridium tetanomorphum
?
-
-
-
-
4.3.1.2
ethylfumarate + NH3
-
6059
Citrobacter amalonaticus
?
-
-
-
-
4.3.1.2
ethylfumarate + NH3
-
6059
Citrobacter amalonaticus YG-1002
?
-
-
-
-
4.3.1.2
ethylfumarate + NH3
-
6059
Clostridium tetanomorphum H1 / ATCC 15920
?
-
-
-
-
4.3.1.2
ethylfumarate + NH3
-
6059
Clostridium tetanomorphum NCIMB 11547
?
-
-
-
-
4.3.1.2
L-threo-3-methylaspartate
r
6059
Clostridium tetanomorphum
mesaconate + NH3
-
6059
Clostridium tetanomorphum
-
4.3.1.2
L-threo-3-methylaspartate
r
6059
Citrobacter amalonaticus
mesaconate + NH3
-
6059
Citrobacter amalonaticus
-
4.3.1.2
L-threo-3-methylaspartate
(2S,3S)-3-methylaspartic acid
6059
Clostridium tetanomorphum
mesaconate + NH3
-
6059
Clostridium tetanomorphum
-
4.3.1.2
L-threo-3-methylaspartate
(2S,3S)-3-methylaspartic acid
6059
Citrobacter amalonaticus
mesaconate + NH3
-
6059
Citrobacter amalonaticus
-
4.3.1.2
L-threo-3-methylaspartate
r
6059
Citrobacter amalonaticus YG-1002
mesaconate + NH3
-
6059
Citrobacter amalonaticus YG-1002
-
4.3.1.2
L-threo-3-methylaspartate
(2S,3S)-3-methylaspartic acid
6059
Citrobacter amalonaticus YG-1002
mesaconate + NH3
-
6059
Citrobacter amalonaticus YG-1002
-
4.3.1.2
L-threo-3-methylaspartate
r
6059
Clostridium tetanomorphum H1 / ATCC 15920
mesaconate + NH3
-
6059
Clostridium tetanomorphum H1 / ATCC 15920
-
4.3.1.2
L-threo-3-methylaspartate
(2S,3S)-3-methylaspartic acid
6059
Clostridium tetanomorphum H1 / ATCC 15920
mesaconate + NH3
-
6059
Clostridium tetanomorphum H1 / ATCC 15920
-
4.3.1.2
L-threo-3-methylaspartate
r
6059
Clostridium tetanomorphum NCIMB 11547
mesaconate + NH3
-
6059
Clostridium tetanomorphum NCIMB 11547
-
4.3.1.2
L-threo-3-methylaspartate
(2S,3S)-3-methylaspartic acid
6059
Clostridium tetanomorphum NCIMB 11547
mesaconate + NH3
-
6059
Clostridium tetanomorphum NCIMB 11547
-
4.3.1.2
L-threo-3-methylaspartate
-
6059
Clostridium tetanomorphum
?
-
-
-
-
4.3.1.2
L-threo-3-methylaspartate
-
6059
Citrobacter amalonaticus
?
-
-
-
-
4.3.1.2
additional information
-
6059
Clostridium tetanomorphum
?
-
-
-
-
4.3.1.2
additional information
-
6059
Citrobacter amalonaticus
?
-
-
-
-
4.3.1.2
additional information
-
6059
Citrobacter amalonaticus YG-1002
?
-
-
-
-
4.3.1.2
additional information
-
6059
Clostridium tetanomorphum H1 / ATCC 15920
?
-
-
-
-
4.3.1.2
additional information
-
6059
Clostridium tetanomorphum NCIMB 11547
?
-
-
-
-
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
4.3.1.2
dimer
2 * 42000, SDS-PAGE
Citrobacter amalonaticus
4.3.1.2
dimer
-
Clostridium tetanomorphum
Temperature Optimum [C] (protein specific)
EC Number
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
4.3.1.2
50
-
maximal activity in the deamination reaction of L-threo-3-methylaspartate
Citrobacter amalonaticus
Temperature Stability [C] (protein specific)
EC Number
Temperature Stability Minimum [C]
Temperature Stability Maximum [C]
Commentary
Organism
4.3.1.2
45
-
0.1 mM potassium phosphate buffer, pH 7.0, 10 min, in the deamination reaction of (2S,3S)-3-methylaspartate, 6% loss of activity
Citrobacter amalonaticus
4.3.1.2
50
-
0.1 mM potassium phosphate buffer, pH 7.0, 10 min, in the deamination reaction of (2S,3S)-3-methylaspartate, 10% loss of activity
Citrobacter amalonaticus
4.3.1.2
55
-
0.1 mM potassium phosphate buffer, pH 7.0, 10 min, in the deamination reaction of (2S,3S)-3-methylaspartate, 44% loss of activity
Citrobacter amalonaticus
4.3.1.2
60
-
0.1 mM potassium phosphate buffer, pH 7.0, 10 min, in the deamination reaction of (2S,3S)-3-methxylaspartate, 96.5% loss of activity
Citrobacter amalonaticus
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
4.3.1.2
8.5
-
maximal activity in TRIS/HCl buffer in the amination reaction of mesaconate
Citrobacter amalonaticus
4.3.1.2
9.7
-
maximal activity in ethanolamine/HCl buffer in the deamination reaction of L-threo-3-methylaspartate
Citrobacter amalonaticus
pH Stability (protein specific)
EC Number
pH Stability
pH Stability Maximum
Commentary
Organism
4.3.1.2
additional information
-
-
Citrobacter amalonaticus
4.3.1.2
5.5
11
30C, 10 min, deamination reaction of L-threo-3-methylaspartate, 14% loss of activity
Citrobacter amalonaticus