BRENDA - Enzyme Database

Cloning, sequencing, and expression in Escherichia coli of the Clostridium tetanomorphum gene encoding beta-methylaspartase and characterization of the recombinant protein

Goda, S.K.; Minton, N.P.; Botting, N.P.; Gani, D.; Biochemistry 31, 10747-10756 (1992)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
4.3.1.2
expression in Escherichia coli
Clostridium tetanomorphum
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
4.3.1.2
K+
-
Clostridium tetanomorphum
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
4.3.1.2
45539
-
2 * 45539, calculation from sequence of amino acid
Clostridium tetanomorphum
4.3.1.2
49000
-
2 * 49000, SDS-PAGE
Clostridium tetanomorphum
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
4.3.1.2
L-threo-3-methylaspartate
Clostridium tetanomorphum
-
?
-
-
-
4.3.1.2
L-threo-3-methylaspartate
Clostridium tetanomorphum H1 / ATCC 15920
-
?
-
-
-
4.3.1.2
additional information
Clostridium tetanomorphum
enzyme is involved in the catabolic pathway for glutamate
?
-
-
-
4.3.1.2
additional information
Clostridium tetanomorphum H1 / ATCC 15920
enzyme is involved in the catabolic pathway for glutamate
?
-
-
-
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
4.3.1.2
Clostridium tetanomorphum
-
; ATCC 15920
-
4.3.1.2
Clostridium tetanomorphum H1 / ATCC 15920
-
-
-
Reaction
EC Number
Reaction
Commentary
Organism
4.3.1.2
L-threo-3-methylaspartate = mesaconate + NH3
mechanism
Clostridium tetanomorphum
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
4.3.1.2
141
-
-
Clostridium tetanomorphum
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4.3.1.2
erythro-3-methylaspartate
-
6057
Clostridium tetanomorphum
?
-
-
-
-
4.3.1.2
erythro-3-methylaspartate
-
6057
Clostridium tetanomorphum H1 / ATCC 15920
?
-
-
-
-
4.3.1.2
L-aspartate
(2S)-aspartate
6057
Clostridium tetanomorphum
fumarate + NH3
-
-
-
-
4.3.1.2
L-aspartate
(2S)-aspartate
6057
Clostridium tetanomorphum H1 / ATCC 15920
fumarate + NH3
-
-
-
-
4.3.1.2
L-threo-3-methylaspartate
r
6057
Clostridium tetanomorphum
mesaconate + NH3
-
6057
Clostridium tetanomorphum
-
4.3.1.2
L-threo-3-methylaspartate
r
6057
Clostridium tetanomorphum H1 / ATCC 15920
mesaconate + NH3
-
6057
Clostridium tetanomorphum H1 / ATCC 15920
-
4.3.1.2
L-threo-3-methylaspartate
-
6057
Clostridium tetanomorphum
?
-
-
-
-
4.3.1.2
L-threo-3-methylaspartate
-
6057
Clostridium tetanomorphum H1 / ATCC 15920
?
-
-
-
-
4.3.1.2
additional information
enzyme is involved in the catabolic pathway for glutamate
6057
Clostridium tetanomorphum
?
-
-
-
-
4.3.1.2
additional information
enzyme is involved in the catabolic pathway for glutamate
6057
Clostridium tetanomorphum H1 / ATCC 15920
?
-
-
-
-
Subunits
EC Number
Subunits
Commentary
Organism
4.3.1.2
dimer
2 * 45539, calculation from sequence of amino acid; 2 * 49000, SDS-PAGE
Clostridium tetanomorphum
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
4.3.1.2
expression in Escherichia coli
Clostridium tetanomorphum
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
4.3.1.2
K+
-
Clostridium tetanomorphum
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
4.3.1.2
45539
-
2 * 45539, calculation from sequence of amino acid
Clostridium tetanomorphum
4.3.1.2
49000
-
2 * 49000, SDS-PAGE
Clostridium tetanomorphum
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
4.3.1.2
L-threo-3-methylaspartate
Clostridium tetanomorphum
-
?
-
-
-
4.3.1.2
L-threo-3-methylaspartate
Clostridium tetanomorphum H1 / ATCC 15920
-
?
-
-
-
4.3.1.2
additional information
Clostridium tetanomorphum
enzyme is involved in the catabolic pathway for glutamate
?
-
-
-
4.3.1.2
additional information
Clostridium tetanomorphum H1 / ATCC 15920
enzyme is involved in the catabolic pathway for glutamate
?
-
-
-
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
4.3.1.2
141
-
-
Clostridium tetanomorphum
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4.3.1.2
erythro-3-methylaspartate
-
6057
Clostridium tetanomorphum
?
-
-
-
-
4.3.1.2
erythro-3-methylaspartate
-
6057
Clostridium tetanomorphum H1 / ATCC 15920
?
-
-
-
-
4.3.1.2
L-aspartate
(2S)-aspartate
6057
Clostridium tetanomorphum
fumarate + NH3
-
-
-
-
4.3.1.2
L-aspartate
(2S)-aspartate
6057
Clostridium tetanomorphum H1 / ATCC 15920
fumarate + NH3
-
-
-
-
4.3.1.2
L-threo-3-methylaspartate
r
6057
Clostridium tetanomorphum
mesaconate + NH3
-
6057
Clostridium tetanomorphum
-
4.3.1.2
L-threo-3-methylaspartate
r
6057
Clostridium tetanomorphum H1 / ATCC 15920
mesaconate + NH3
-
6057
Clostridium tetanomorphum H1 / ATCC 15920
-
4.3.1.2
L-threo-3-methylaspartate
-
6057
Clostridium tetanomorphum
?
-
-
-
-
4.3.1.2
L-threo-3-methylaspartate
-
6057
Clostridium tetanomorphum H1 / ATCC 15920
?
-
-
-
-
4.3.1.2
additional information
enzyme is involved in the catabolic pathway for glutamate
6057
Clostridium tetanomorphum
?
-
-
-
-
4.3.1.2
additional information
enzyme is involved in the catabolic pathway for glutamate
6057
Clostridium tetanomorphum H1 / ATCC 15920
?
-
-
-
-
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
4.3.1.2
dimer
2 * 45539, calculation from sequence of amino acid; 2 * 49000, SDS-PAGE
Clostridium tetanomorphum