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Literature summary extracted from

  • Hsiang, M.W.; Bright, H.J.
    beta-methylaspartase from Clostridium tetanomorphum (1969), Methods Enzymol., 13, 347-353.
No PubMed abstract available

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
4.3.1.2 crystallized from ammonium sulfate Clostridium tetanomorphum

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.3.1.2 N-ethylmaleimide
-
 Clostridium tetanomorphum

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.3.1.2 Co2+
-
 Clostridium tetanomorphum
4.3.1.2 Mg2+
-
 Clostridium tetanomorphum
4.3.1.2 Mn2+
-
 Clostridium tetanomorphum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.3.1.2 100000
-
 equilibrium ultracentrifugation Clostridium tetanomorphum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.3.1.2 L-threo-3-methylaspartate Bacterium cadaveris
-
 ?
-
 ?
4.3.1.2 L-threo-3-methylaspartate Clostridium tetanomorphum
-
 ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.3.1.2 Bacterium cadaveris
-
-
-
4.3.1.2 Clostridium tetanomorphum
-
-
-
4.3.1.2 Clostridium tetanomorphum H1 / ATCC 15920
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.3.1.2
-
 Clostridium tetanomorphum

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.3.1.2 280
-
-
 Clostridium tetanomorphum

Storage Stability

EC Number Storage Stability Organism
4.3.1.2 0-3°C, in 0.5 M tetramethylammonium chloride, pH 6.8 for many months, with little loss of activity Clostridium tetanomorphum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.3.1.2 beta-ethylaspartate
-
 Bacterium cadaveris ?
-
 ?
4.3.1.2 beta-ethylaspartate
-
 Clostridium tetanomorphum ?
-
 ?
4.3.1.2 beta-ethylaspartate
-
 Clostridium tetanomorphum H1 / ATCC 15920 ?
-
 ?
4.3.1.2 beta-isopropylaspartate
-
 Clostridium tetanomorphum ?
-
 ?
4.3.1.2 beta-propylaspartate
-
 Clostridium tetanomorphum ?
-
 ?
4.3.1.2 erythro-3-methylaspartate
-
 Bacterium cadaveris ?
-
 ?
4.3.1.2 erythro-3-methylaspartate erythro-beta-methylaspartate Clostridium tetanomorphum ?
-
 ?
4.3.1.2 erythro-3-methylaspartate erythro-beta-methylaspartate Clostridium tetanomorphum H1 / ATCC 15920 ?
-
 ?
4.3.1.2 L-aspartate
-
 Clostridium tetanomorphum fumarate + NH3
-
 ?
4.3.1.2 L-aspartate
-
 Clostridium tetanomorphum H1 / ATCC 15920 fumarate + NH3
-
 ?
4.3.1.2 L-threo-3-methylaspartate r Bacterium cadaveris mesaconate + NH3
-
 ?
4.3.1.2 L-threo-3-methylaspartate r Clostridium tetanomorphum mesaconate + NH3
-
 ?
4.3.1.2 L-threo-3-methylaspartate threo-beta-methyl-L-aspartate Clostridium tetanomorphum mesaconate + NH3
-
 ?
4.3.1.2 L-threo-3-methylaspartate r Clostridium tetanomorphum H1 / ATCC 15920 mesaconate + NH3
-
 ?
4.3.1.2 L-threo-3-methylaspartate threo-beta-methyl-L-aspartate Clostridium tetanomorphum H1 / ATCC 15920 mesaconate + NH3
-
 ?
4.3.1.2 L-threo-3-methylaspartate
-
 Bacterium cadaveris ?
-
 ?
4.3.1.2 L-threo-3-methylaspartate
-
 Clostridium tetanomorphum ?
-
 ?

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.3.1.2 additional information
-
 additional information
-
 Clostridium tetanomorphum
4.3.1.2 467
-
 L-threo-3-methylaspartate pH 9.7 Clostridium tetanomorphum