BRENDA - Enzyme Database

beta-methylaspartase from Clostridium tetanomorphum

Hsiang, M.W.; Bright, H.J.; Methods Enzymol. 13, 347-353 (1969)
No PubMed abstract available

Data extracted from this reference:

Crystallization (Commentary)
EC Number
Crystallization
Organism
4.3.1.2
crystallized from ammonium sulfate
Clostridium tetanomorphum
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
4.3.1.2
N-ethylmaleimide
-
Clostridium tetanomorphum
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
4.3.1.2
Co2+
-
Clostridium tetanomorphum
4.3.1.2
Mg2+
-
Clostridium tetanomorphum
4.3.1.2
Mn2+
-
Clostridium tetanomorphum
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
4.3.1.2
100000
-
equilibrium ultracentrifugation
Clostridium tetanomorphum
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
4.3.1.2
L-threo-3-methylaspartate
Bacterium cadaveris
-
?
-
-
-
4.3.1.2
L-threo-3-methylaspartate
Clostridium tetanomorphum
-
?
-
-
-
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
4.3.1.2
Bacterium cadaveris
-
-
-
4.3.1.2
Clostridium tetanomorphum
-
-
-
4.3.1.2
Clostridium tetanomorphum H1 / ATCC 15920
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
4.3.1.2
-
Clostridium tetanomorphum
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
4.3.1.2
280
-
-
Clostridium tetanomorphum
Storage Stability
EC Number
Storage Stability
Organism
4.3.1.2
0-3°C, in 0.5 M tetramethylammonium chloride, pH 6.8 for many months, with little loss of activity
Clostridium tetanomorphum
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4.3.1.2
beta-ethylaspartate
-
6051
Bacterium cadaveris
?
-
-
-
-
4.3.1.2
beta-ethylaspartate
-
6051
Clostridium tetanomorphum
?
-
-
-
-
4.3.1.2
beta-ethylaspartate
-
6051
Clostridium tetanomorphum H1 / ATCC 15920
?
-
-
-
-
4.3.1.2
beta-isopropylaspartate
-
6051
Clostridium tetanomorphum
?
-
-
-
-
4.3.1.2
beta-propylaspartate
-
6051
Clostridium tetanomorphum
?
-
-
-
-
4.3.1.2
erythro-3-methylaspartate
-
6051
Bacterium cadaveris
?
-
-
-
-
4.3.1.2
erythro-3-methylaspartate
erythro-beta-methylaspartate
6051
Clostridium tetanomorphum
?
-
-
-
-
4.3.1.2
erythro-3-methylaspartate
erythro-beta-methylaspartate
6051
Clostridium tetanomorphum H1 / ATCC 15920
?
-
-
-
-
4.3.1.2
L-aspartate
-
6051
Clostridium tetanomorphum
fumarate + NH3
-
-
-
-
4.3.1.2
L-aspartate
-
6051
Clostridium tetanomorphum H1 / ATCC 15920
fumarate + NH3
-
-
-
-
4.3.1.2
L-threo-3-methylaspartate
r
6051
Bacterium cadaveris
mesaconate + NH3
-
6051
Bacterium cadaveris
-
4.3.1.2
L-threo-3-methylaspartate
r
6051
Clostridium tetanomorphum
mesaconate + NH3
-
6051
Clostridium tetanomorphum
-
4.3.1.2
L-threo-3-methylaspartate
threo-beta-methyl-L-aspartate
6051
Clostridium tetanomorphum
mesaconate + NH3
-
6051
Clostridium tetanomorphum
-
4.3.1.2
L-threo-3-methylaspartate
r
6051
Clostridium tetanomorphum H1 / ATCC 15920
mesaconate + NH3
-
6051
Clostridium tetanomorphum H1 / ATCC 15920
-
4.3.1.2
L-threo-3-methylaspartate
threo-beta-methyl-L-aspartate
6051
Clostridium tetanomorphum H1 / ATCC 15920
mesaconate + NH3
-
6051
Clostridium tetanomorphum H1 / ATCC 15920
-
4.3.1.2
L-threo-3-methylaspartate
-
6051
Bacterium cadaveris
?
-
-
-
-
4.3.1.2
L-threo-3-methylaspartate
-
6051
Clostridium tetanomorphum
?
-
-
-
-
Turnover Number [1/s]
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
4.3.1.2
additional information
-
additional information
-
Clostridium tetanomorphum
4.3.1.2
467
-
L-threo-3-methylaspartate
pH 9.7
Clostridium tetanomorphum
Crystallization (Commentary) (protein specific)
EC Number
Crystallization
Organism
4.3.1.2
crystallized from ammonium sulfate
Clostridium tetanomorphum
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
4.3.1.2
N-ethylmaleimide
-
Clostridium tetanomorphum
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
4.3.1.2
Co2+
-
Clostridium tetanomorphum
4.3.1.2
Mg2+
-
Clostridium tetanomorphum
4.3.1.2
Mn2+
-
Clostridium tetanomorphum
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
4.3.1.2
100000
-
equilibrium ultracentrifugation
Clostridium tetanomorphum
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
4.3.1.2
L-threo-3-methylaspartate
Bacterium cadaveris
-
?
-
-
-
4.3.1.2
L-threo-3-methylaspartate
Clostridium tetanomorphum
-
?
-
-
-
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
4.3.1.2
-
Clostridium tetanomorphum
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
4.3.1.2
280
-
-
Clostridium tetanomorphum
Storage Stability (protein specific)
EC Number
Storage Stability
Organism
4.3.1.2
0-3°C, in 0.5 M tetramethylammonium chloride, pH 6.8 for many months, with little loss of activity
Clostridium tetanomorphum
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4.3.1.2
beta-ethylaspartate
-
6051
Bacterium cadaveris
?
-
-
-
-
4.3.1.2
beta-ethylaspartate
-
6051
Clostridium tetanomorphum
?
-
-
-
-
4.3.1.2
beta-ethylaspartate
-
6051
Clostridium tetanomorphum H1 / ATCC 15920
?
-
-
-
-
4.3.1.2
beta-isopropylaspartate
-
6051
Clostridium tetanomorphum
?
-
-
-
-
4.3.1.2
beta-propylaspartate
-
6051
Clostridium tetanomorphum
?
-
-
-
-
4.3.1.2
erythro-3-methylaspartate
-
6051
Bacterium cadaveris
?
-
-
-
-
4.3.1.2
erythro-3-methylaspartate
erythro-beta-methylaspartate
6051
Clostridium tetanomorphum
?
-
-
-
-
4.3.1.2
erythro-3-methylaspartate
erythro-beta-methylaspartate
6051
Clostridium tetanomorphum H1 / ATCC 15920
?
-
-
-
-
4.3.1.2
L-aspartate
-
6051
Clostridium tetanomorphum
fumarate + NH3
-
-
-
-
4.3.1.2
L-aspartate
-
6051
Clostridium tetanomorphum H1 / ATCC 15920
fumarate + NH3
-
-
-
-
4.3.1.2
L-threo-3-methylaspartate
r
6051
Bacterium cadaveris
mesaconate + NH3
-
6051
Bacterium cadaveris
-
4.3.1.2
L-threo-3-methylaspartate
r
6051
Clostridium tetanomorphum
mesaconate + NH3
-
6051
Clostridium tetanomorphum
-
4.3.1.2
L-threo-3-methylaspartate
threo-beta-methyl-L-aspartate
6051
Clostridium tetanomorphum
mesaconate + NH3
-
6051
Clostridium tetanomorphum
-
4.3.1.2
L-threo-3-methylaspartate
r
6051
Clostridium tetanomorphum H1 / ATCC 15920
mesaconate + NH3
-
6051
Clostridium tetanomorphum H1 / ATCC 15920
-
4.3.1.2
L-threo-3-methylaspartate
threo-beta-methyl-L-aspartate
6051
Clostridium tetanomorphum H1 / ATCC 15920
mesaconate + NH3
-
6051
Clostridium tetanomorphum H1 / ATCC 15920
-
4.3.1.2
L-threo-3-methylaspartate
-
6051
Bacterium cadaveris
?
-
-
-
-
4.3.1.2
L-threo-3-methylaspartate
-
6051
Clostridium tetanomorphum
?
-
-
-
-
Turnover Number [1/s] (protein specific)
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
4.3.1.2
additional information
-
additional information
-
Clostridium tetanomorphum
4.3.1.2
467
-
L-threo-3-methylaspartate
pH 9.7
Clostridium tetanomorphum