EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
4.3.1.13 | beta-chloro-L-alanine | irreversible | Rattus norvegicus | |
4.3.1.13 | L-Albizziin | 20 mM, 20% inhibition | Rattus norvegicus | |
4.3.1.13 | L-glutamine | 20 mM, 60% inhibition | Rattus norvegicus |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
4.3.1.13 | 17 | - |
O-Carbamoyl-L-serine | - |
Rattus norvegicus |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
4.3.1.13 | O-Carbamoyl-L-serine | Rattus norvegicus | - |
? | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
4.3.1.13 | Rattus norvegicus | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
4.3.1.13 | - |
Rattus norvegicus |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
4.3.1.13 | liver | - |
Rattus norvegicus | - |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
4.3.1.13 | 0.49 | - |
- |
Rattus norvegicus |
EC Number | Storage Stability | Organism |
---|---|---|
4.3.1.13 | -20°C, no loss of activity after 2 weeks in 20% glycerol containing 0.1 mM pyridoxal 5-phosphate, 1 mM 2-mercaptoethanol and 10 mM potassium phosphate bufer, pH 7.2 | Rattus norvegicus |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
4.3.1.13 | O-carbamoyl-L-serine | - |
Rattus norvegicus | pyruvate + 2 NH3 + CO2 | - |
? | |
4.3.1.13 | O-Carbamoyl-L-serine | - |
Rattus norvegicus | ? | - |
? | |
4.3.1.13 | S-carbamoyl-L-cysteine | - |
Rattus norvegicus | pyruvate + ? | 10% pyruvate formation of that towards O-carbamoyl-L-serine | ? |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
4.3.1.13 | 37 | - |
enzyme assay at | Rattus norvegicus |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
4.3.1.13 | 7.8 | 8.8 | in 0.1 M sodium borate buffer | Rattus norvegicus |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
4.3.1.13 | pyridoxal 5'-phosphate | omission leads to 60% decrease of activity | Rattus norvegicus |