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Mapping the mechanism-based modification sites in L-aspartase from Escherichia coli

Giorgianni, F.; Beranova, S.; Wesdemiotis, C.; Viola, R.E.; Arch. Biochem. Biophys. 341, 329-336 (1997)

Data extracted from this reference:

Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
4.3.1.1
aspartate beta-semialdehyde
-
Escherichia coli
4.3.1.1
fumaric acid aldehyde
-
Escherichia coli
4.3.1.1
fumaric acid aldehyde ethyl ester
-
Escherichia coli
4.3.1.1
additional information
-
Escherichia coli
4.3.1.1
o-phospho-D-serine
competitive
Escherichia coli
4.3.1.1
O-phospho-L-serine
competitive
Escherichia coli
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
4.3.1.1
0.54
-
L-aspartate
C273A mutant, pH 7.0
Escherichia coli
4.3.1.1
0.6
-
L-aspartate
C140S/C273A mutant, pH 7.0
Escherichia coli
4.3.1.1
1.2
-
L-aspartate
wild-type, pH 7.0
Escherichia coli
4.3.1.1
1.3
-
L-aspartate
C273S mutant, pH 7.0
Escherichia coli
4.3.1.1
12
-
L-aspartate
K139I mutant, pH 7.0
Escherichia coli
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
4.3.1.1
Escherichia coli
-
C273S, C273A, C140S/C273A, K139I (mutants)
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4.3.1.1
L-aspartate
r
6028
Escherichia coli
fumarate + NH3
-
6028
Escherichia coli
-
4.3.1.1
L-aspartate
absolutely specific
6028
Escherichia coli
fumarate + NH3
-
6028
Escherichia coli
-
4.3.1.1
L-aspartate
natural substrate
6028
Escherichia coli
fumarate + NH3
-
6028
Escherichia coli
-
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
4.3.1.1
30
-
-
Escherichia coli
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
4.3.1.1
aspartate beta-semialdehyde
-
Escherichia coli
4.3.1.1
fumaric acid aldehyde
-
Escherichia coli
4.3.1.1
fumaric acid aldehyde ethyl ester
-
Escherichia coli
4.3.1.1
additional information
-
Escherichia coli
4.3.1.1
o-phospho-D-serine
competitive
Escherichia coli
4.3.1.1
O-phospho-L-serine
competitive
Escherichia coli
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
4.3.1.1
0.54
-
L-aspartate
C273A mutant, pH 7.0
Escherichia coli
4.3.1.1
0.6
-
L-aspartate
C140S/C273A mutant, pH 7.0
Escherichia coli
4.3.1.1
1.2
-
L-aspartate
wild-type, pH 7.0
Escherichia coli
4.3.1.1
1.3
-
L-aspartate
C273S mutant, pH 7.0
Escherichia coli
4.3.1.1
12
-
L-aspartate
K139I mutant, pH 7.0
Escherichia coli
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4.3.1.1
L-aspartate
r
6028
Escherichia coli
fumarate + NH3
-
6028
Escherichia coli
-
4.3.1.1
L-aspartate
absolutely specific
6028
Escherichia coli
fumarate + NH3
-
6028
Escherichia coli
-
4.3.1.1
L-aspartate
natural substrate
6028
Escherichia coli
fumarate + NH3
-
6028
Escherichia coli
-
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
4.3.1.1
30
-
-
Escherichia coli