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Literature summary extracted from

  • Rollan, G.C.; Manca de Nadra, M.C.; Pesce de Ruiz Holgado, A.A.; Oliver, G.
    Aspartate metabolism in Lactobacillus murinus CNRS 313. I. Aspartase (1985), J. Gen. Appl. Microbiol., 31, 403-409.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.3.1.1 HgCl2 1 mM, 89.5% inhibition Ligilactobacillus murinus
4.3.1.1 additional information
-
 Ligilactobacillus murinus
4.3.1.1 NaCN 1 mM, 42% inhibition Ligilactobacillus murinus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.3.1.1 1.5
-
 L-aspartate Tris buffer, pH 7.0 Bacterium cadaveris
4.3.1.1 20
-
 L-aspartate phosphate buffer, pH 7.0 Bacterium cadaveris
4.3.1.1 30
-
 L-aspartate phosphate buffer, pH 6.8 Bacterium cadaveris
4.3.1.1 37
-
 L-aspartate pH 7.5 Ligilactobacillus murinus
4.3.1.1 90
-
 L-aspartate pH 7.0 Ligilactobacillus murinus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.3.1.1 additional information Ligilactobacillus murinus one of the enzymes of the aspartate metabolism in lactic acid bacteria ?
-
 ?
4.3.1.1 additional information Ligilactobacillus murinus CNRZ 313 one of the enzymes of the aspartate metabolism in lactic acid bacteria ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.3.1.1 Bacterium cadaveris
-
-
-
4.3.1.1 Klebsiella aerogenes
-
-
-
4.3.1.1 Ligilactobacillus murinus
-
 CNRZ 313
-
4.3.1.1 Ligilactobacillus murinus CNRZ 313
-
 CNRZ 313
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.3.1.1 additional information
-
-
 Ligilactobacillus murinus

Storage Stability

EC Number Storage Stability Organism
4.3.1.1 -70°C, stable for 1 month without addition of salts or glycerol Ligilactobacillus murinus
4.3.1.1 4°C, loss of activity after 1 month independently of the presence of glycerol or MgCl2 Ligilactobacillus murinus
4.3.1.1 enzyme activity can be maintained by freezing up to 6 months in the ammonium sulfate precipitate Klebsiella aerogenes
4.3.1.1 additional information Ligilactobacillus murinus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.3.1.1 L-aspartate r Klebsiella aerogenes fumarate + NH3
-
 ?
4.3.1.1 L-aspartate r Ligilactobacillus murinus fumarate + NH3
-
 ?
4.3.1.1 L-aspartate natural substrate Klebsiella aerogenes fumarate + NH3
-
 ?
4.3.1.1 L-aspartate natural substrate Bacterium cadaveris fumarate + NH3
-
 ?
4.3.1.1 L-aspartate natural substrate Ligilactobacillus murinus fumarate + NH3
-
 ?
4.3.1.1 L-aspartate r Ligilactobacillus murinus CNRZ 313 fumarate + NH3
-
 ?
4.3.1.1 L-aspartate natural substrate Ligilactobacillus murinus CNRZ 313 fumarate + NH3
-
 ?
4.3.1.1 additional information one of the enzymes of the aspartate metabolism in lactic acid bacteria Ligilactobacillus murinus ?
-
 ?
4.3.1.1 additional information one of the enzymes of the aspartate metabolism in lactic acid bacteria Ligilactobacillus murinus CNRZ 313 ?
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.3.1.1 32
-
-
 Ligilactobacillus murinus

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
4.3.1.1 additional information
-
-
 Ligilactobacillus murinus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
4.3.1.1 additional information
-
-
 Ligilactobacillus murinus
4.3.1.1 50
-
 37% loss of activity after 5 min Ligilactobacillus murinus
4.3.1.1 55
-
 85% loss of activity after 5 min Ligilactobacillus murinus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.3.1.1 additional information
-
-
 Ligilactobacillus murinus