BRENDA - Enzyme Database show

Aspartate ammonia-lyase

Tokushige, M.; Methods Enzymol. 113, 618-627 (1985)

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
4.3.1.1
acid anhydride
activates
Escherichia coli
4.3.1.1
alpha-methyl-DL-aspartate
activates
Escherichia coli
4.3.1.1
N-hydroxysuccinimide acetate
activates
Escherichia coli
4.3.1.1
subtilisin BPN'
activates
Escherichia coli
4.3.1.1
Trypsin
enzyme is activated several-fold by limited treatment
Escherichia coli
Crystallization (Commentary)
EC Number
Crystallization
Organism
4.3.1.1
-
Pseudomonas fluorescens
General Stability
EC Number
General Stability
Organism
4.3.1.1
enzyme is fairly stable in the presence of high concentrations of ammonium sulfate, potassium phosphate, and KCl
Escherichia coli
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
4.3.1.1
5,5'-dithiobis(2-nitrobenzoate)
-
Escherichia coli
4.3.1.1
aspartate beta-semialdehyde
i.e. aspartic beta-semialdehyde.Inactivates as an active-site directed agent
Escherichia coli
4.3.1.1
D-Aspartate
competitive
Escherichia coli
4.3.1.1
diethyldicarbonate
reactivated by hydroxylamine
Escherichia coli
4.3.1.1
EDTA
partial
Escherichia coli
4.3.1.1
p-hydroxymercuribenzoate
-
Escherichia coli
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
4.3.1.1
2.4
-
L-aspartate
in the presence of 3 mM MgCl2, pH 9.0
Pseudomonas fluorescens
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
4.3.1.1
Mg2+
activates at the alkine pH
Escherichia coli
4.3.1.1
Mg2+
activates at the alkine pH
Hafnia alvei
4.3.1.1
Mn2+
activates at the alkine pH
Escherichia coli
4.3.1.1
Mn2+
-
Hafnia alvei
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
4.3.1.1
173000
-
sedimentation equilibrium analysis
Pseudomonas fluorescens
4.3.1.1
193000
-
sedimentation equilibrium analysis
Escherichia coli
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
4.3.1.1
additional information
Escherichia coli
enzyme synthesis is subject to catabolite repression by glucose and is suppressed under aerobic conditions
?
-
-
-
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
4.3.1.1
Escherichia coli
-
-
-
4.3.1.1
Hafnia alvei
-
-
-
4.3.1.1
Pseudomonas fluorescens
-
IFO 3081
-
Purification (Commentary)
EC Number
Commentary
Organism
4.3.1.1
-
Pseudomonas fluorescens
4.3.1.1
-
Escherichia coli
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [Ámol/min/mg]
Specific Activity Maximum [Ámol/min/mg]
Commentary
Organism
4.3.1.1
68.5
-
-
Escherichia coli
4.3.1.1
229
-
-
Pseudomonas fluorescens
Storage Stability
EC Number
Storage Stability
Organism
4.3.1.1
2-mercaptoethanol and dithiothreitol protect enzyme against inactivation during storage
Escherichia coli
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4.3.1.1
fumarate + hydroxylamine
substrates for reverse reaction
6009
Escherichia coli
?
-
-
-
-
4.3.1.1
fumarate + NH4Cl
substrates for reverse reaction
6009
Escherichia coli
?
-
-
-
-
4.3.1.1
L-aspartate
r
6009
Escherichia coli
fumarate + NH3
-
6009
Escherichia coli
-
4.3.1.1
L-aspartate
r
6009
Pseudomonas fluorescens
fumarate + NH3
-
6009
Pseudomonas fluorescens
-
4.3.1.1
L-aspartate
r
6009
Hafnia alvei
fumarate + NH3
-
6009
Hafnia alvei
-
4.3.1.1
L-aspartate
absolutely specific
6009
Escherichia coli
fumarate + NH3
-
6009
Escherichia coli
-
4.3.1.1
L-aspartate
natural substrate
6009
Escherichia coli
fumarate + NH3
-
6009
Escherichia coli
-
4.3.1.1
L-aspartate
natural substrate
6009
Pseudomonas fluorescens
fumarate + NH3
-
6009
Pseudomonas fluorescens
-
4.3.1.1
L-aspartate
natural substrate
6009
Hafnia alvei
fumarate + NH3
-
6009
Hafnia alvei
-
4.3.1.1
additional information
enzyme synthesis is subject to catabolite repression by glucose and is suppressed under aerobic conditions
6009
Escherichia coli
?
-
-
-
-
Temperature Stability [░C]
EC Number
Temperature Stability Minimum [░C]
Temperature Stability Maximum [░C]
Commentary
Organism
4.3.1.1
additional information
-
-
Escherichia coli
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
4.3.1.1
7.8
-
in the absence of MgCl2
Escherichia coli
4.3.1.1
8.8
-
native enzyme in the presence of MgCl2
Escherichia coli
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
4.3.1.1
acid anhydride
activates
Escherichia coli
4.3.1.1
alpha-methyl-DL-aspartate
activates
Escherichia coli
4.3.1.1
N-hydroxysuccinimide acetate
activates
Escherichia coli
4.3.1.1
subtilisin BPN'
activates
Escherichia coli
4.3.1.1
Trypsin
enzyme is activated several-fold by limited treatment
Escherichia coli
Crystallization (Commentary) (protein specific)
EC Number
Crystallization
Organism
4.3.1.1
-
Pseudomonas fluorescens
General Stability (protein specific)
EC Number
General Stability
Organism
4.3.1.1
enzyme is fairly stable in the presence of high concentrations of ammonium sulfate, potassium phosphate, and KCl
Escherichia coli
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
4.3.1.1
5,5'-dithiobis(2-nitrobenzoate)
-
Escherichia coli
4.3.1.1
aspartate beta-semialdehyde
i.e. aspartic beta-semialdehyde.Inactivates as an active-site directed agent
Escherichia coli
4.3.1.1
D-Aspartate
competitive
Escherichia coli
4.3.1.1
diethyldicarbonate
reactivated by hydroxylamine
Escherichia coli
4.3.1.1
EDTA
partial
Escherichia coli
4.3.1.1
p-hydroxymercuribenzoate
-
Escherichia coli
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
4.3.1.1
2.4
-
L-aspartate
in the presence of 3 mM MgCl2, pH 9.0
Pseudomonas fluorescens
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
4.3.1.1
Mg2+
activates at the alkine pH
Escherichia coli
4.3.1.1
Mg2+
activates at the alkine pH
Hafnia alvei
4.3.1.1
Mn2+
activates at the alkine pH
Escherichia coli
4.3.1.1
Mn2+
-
Hafnia alvei
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
4.3.1.1
173000
-
sedimentation equilibrium analysis
Pseudomonas fluorescens
4.3.1.1
193000
-
sedimentation equilibrium analysis
Escherichia coli
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
4.3.1.1
additional information
Escherichia coli
enzyme synthesis is subject to catabolite repression by glucose and is suppressed under aerobic conditions
?
-
-
-
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
4.3.1.1
-
Pseudomonas fluorescens
4.3.1.1
-
Escherichia coli
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [Ámol/min/mg]
Specific Activity Maximum [Ámol/min/mg]
Commentary
Organism
4.3.1.1
68.5
-
-
Escherichia coli
4.3.1.1
229
-
-
Pseudomonas fluorescens
Storage Stability (protein specific)
EC Number
Storage Stability
Organism
4.3.1.1
2-mercaptoethanol and dithiothreitol protect enzyme against inactivation during storage
Escherichia coli
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4.3.1.1
fumarate + hydroxylamine
substrates for reverse reaction
6009
Escherichia coli
?
-
-
-
-
4.3.1.1
fumarate + NH4Cl
substrates for reverse reaction
6009
Escherichia coli
?
-
-
-
-
4.3.1.1
L-aspartate
r
6009
Escherichia coli
fumarate + NH3
-
6009
Escherichia coli
-
4.3.1.1
L-aspartate
r
6009
Pseudomonas fluorescens
fumarate + NH3
-
6009
Pseudomonas fluorescens
-
4.3.1.1
L-aspartate
r
6009
Hafnia alvei
fumarate + NH3
-
6009
Hafnia alvei
-
4.3.1.1
L-aspartate
absolutely specific
6009
Escherichia coli
fumarate + NH3
-
6009
Escherichia coli
-
4.3.1.1
L-aspartate
natural substrate
6009
Escherichia coli
fumarate + NH3
-
6009
Escherichia coli
-
4.3.1.1
L-aspartate
natural substrate
6009
Pseudomonas fluorescens
fumarate + NH3
-
6009
Pseudomonas fluorescens
-
4.3.1.1
L-aspartate
natural substrate
6009
Hafnia alvei
fumarate + NH3
-
6009
Hafnia alvei
-
4.3.1.1
additional information
enzyme synthesis is subject to catabolite repression by glucose and is suppressed under aerobic conditions
6009
Escherichia coli
?
-
-
-
-
Temperature Stability [░C] (protein specific)
EC Number
Temperature Stability Minimum [░C]
Temperature Stability Maximum [░C]
Commentary
Organism
4.3.1.1
additional information
-
-
Escherichia coli
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
4.3.1.1
7.8
-
in the absence of MgCl2
Escherichia coli
4.3.1.1
8.8
-
native enzyme in the presence of MgCl2
Escherichia coli