BRENDA - Enzyme Database

The enzymic elimination of ammonia

Hanson, K.R.; Havir, E.A.; The Enzymes, 3rd Ed. (Boyer, P. D. , ed. ) 7, 75-166 (1972)
No PubMed abstract available

Data extracted from this reference:

General Stability
EC Number
General Stability
Organism
4.3.1.1
irreversible inactivation during prolonged centrifugation
Bacterium cadaveris
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
4.3.1.2
p-mercuribenzoate
-
Clostridium tetanomorphum
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
4.3.1.3
additional information
-
additional information
-
Pseudomonas fluorescens
4.3.1.3
additional information
-
additional information
-
Pseudomonas sp.
4.3.1.3
additional information
-
additional information
-
Rattus norvegicus
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
4.3.1.1
180000
-
sucrose density gradient centrifugation
Bacterium cadaveris
4.3.1.2
100000
-
sedimentation equilibrium
Clostridium tetanomorphum
4.3.1.3
54000
-
4 * 54000, sedimentation in the presence of 6 M guanidine hypochloride and mercaptoethanol
Comamonas testosteroni
4.3.1.3
189000
-
sucrose density gradient centrifugation
Pseudomonas fluorescens
4.3.1.3
200000
-
sedimentation equilibrium
Comamonas testosteroni
4.3.1.3
204000
-
sucrose density gradient centrifugation
toad
4.3.1.3
210000
-
sucrose density gradient centrifugation
Pseudomonas aeruginosa
4.3.1.3
210000
-
-
Pseudomonas sp.
4.3.1.3
218000
-
sucrose density gradient centrifugation
Pseudomonas fluorescens
4.3.1.3
220000
-
sucrose density gradient centrifugation
Bacillus subtilis
4.3.1.3
226000
-
sucrose density gradient centrifugation
Rattus norvegicus
4.3.1.3
243000
-
sucrose density gradient centrifugation
Homo sapiens
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
4.3.1.2
L-threo-3-methylaspartate
Bacterium cadaveris
-
?
-
-
-
4.3.1.2
L-threo-3-methylaspartate
Clostridium tetanomorphum
-
?
-
-
-
4.3.1.2
L-threo-3-methylaspartate
Clostridium tetanomorphum H1 / ATCC 15920
-
?
-
-
-
4.3.1.3
additional information
Rattus norvegicus
-
?
-
-
-
4.3.1.3
additional information
Pseudomonas fluorescens
-
?
-
-
-
4.3.1.3
additional information
Homo sapiens
deficiency of enzyme results in histidinemia
?
-
-
-
4.3.1.3
additional information
Pseudomonas fluorescens A.3.12
-
?
-
-
-
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
4.3.1.1
Anas platyrhynchos
-
-
-
4.3.1.1
Bacterium cadaveris
-
ATCC 9760
-
4.3.1.1
Escherichia coli
-
-
-
4.3.1.1
Frog
-
-
-
4.3.1.1
Gallus gallus
-
-
-
4.3.1.1
shark
-
-
-
4.3.1.1
Yersinia pestis
-
-
-
4.3.1.2
Bacterium cadaveris
-
-
-
4.3.1.2
Clostridium tetanomorphum
-
-
-
4.3.1.2
Clostridium tetanomorphum H1 / ATCC 15920
-
-
-
4.3.1.3
Bacillus subtilis
-
-
-
4.3.1.3
Comamonas testosteroni
-
-
-
4.3.1.3
Homo sapiens
-
-
-
4.3.1.3
Klebsiella aerogenes
-
-
-
4.3.1.3
Pseudomonas aeruginosa
-
-
-
4.3.1.3
Pseudomonas fluorescens
-
A.3.12; mutant from ATCC 11250
-
4.3.1.3
Pseudomonas fluorescens A.3.12
-
A.3.12
-
4.3.1.3
Pseudomonas sp.
-
ATCC 112996; ATCC 11,299b
-
4.3.1.3
Rattus norvegicus
-
-
-
4.3.1.3
Salmonella enterica subsp. enterica serovar Typhimurium
-
-
-
4.3.1.3
toad
-
-
-
Reaction
EC Number
Reaction
Commentary
Organism
4.3.1.1
L-aspartate = fumarate + NH3
mechanism
Escherichia coli
4.3.1.2
L-threo-3-methylaspartate = mesaconate + NH3
carbon mechanism
Bacterium cadaveris
4.3.1.2
L-threo-3-methylaspartate = mesaconate + NH3
carbon mechanism
Clostridium tetanomorphum
4.3.1.3
L-histidine = urocanate + NH3
mechanism
Bacillus subtilis
4.3.1.3
L-histidine = urocanate + NH3
mechanism
Comamonas testosteroni
4.3.1.3
L-histidine = urocanate + NH3
mechanism
Homo sapiens
4.3.1.3
L-histidine = urocanate + NH3
mechanism
Klebsiella aerogenes
4.3.1.3
L-histidine = urocanate + NH3
mechanism
Pseudomonas aeruginosa
4.3.1.3
L-histidine = urocanate + NH3
mechanism
Pseudomonas fluorescens
4.3.1.3
L-histidine = urocanate + NH3
mechanism
Pseudomonas sp.
4.3.1.3
L-histidine = urocanate + NH3
mechanism
Rattus norvegicus
4.3.1.3
L-histidine = urocanate + NH3
mechanism
Salmonella enterica subsp. enterica serovar Typhimurium
4.3.1.3
L-histidine = urocanate + NH3
mechanism
toad
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
4.3.1.1
liver
-
Anas platyrhynchos
-
4.3.1.1
liver
-
Frog
-
4.3.1.1
liver
-
Gallus gallus
-
4.3.1.1
liver
-
shark
-
4.3.1.3
liver
-
Homo sapiens
-
4.3.1.3
liver
-
toad
-
4.3.1.3
liver
-
Rattus norvegicus
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
4.3.1.3
additional information
-
-
Bacillus subtilis
4.3.1.3
additional information
-
-
Pseudomonas fluorescens
4.3.1.3
additional information
-
-
Pseudomonas sp.
4.3.1.3
additional information
-
-
Rattus norvegicus
4.3.1.3
3.4
-
37°C, pH 9.0, 3.3 mM L-histidine
Rattus norvegicus
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4.3.1.1
fumarate + hydroxylamine
-
5997
Escherichia coli
?
-
-
-
-
4.3.1.1
fumarate + hydroxylamine
-
5997
Bacterium cadaveris
?
-
-
-
-
4.3.1.1
L-aspartate
r
5997
Gallus gallus
fumarate + NH3
-
5997
Gallus gallus
-
4.3.1.1
L-aspartate
r
5997
Escherichia coli
fumarate + NH3
-
5997
Escherichia coli
-
4.3.1.1
L-aspartate
r
5997
Frog
fumarate + NH3
-
5997
Frog
-
4.3.1.1
L-aspartate
r
5997
Anas platyrhynchos
fumarate + NH3
-
5997
Anas platyrhynchos
-
4.3.1.1
L-aspartate
r
5997
Yersinia pestis
fumarate + NH3
-
5997
Yersinia pestis
-
4.3.1.1
L-aspartate
r
5997
shark
fumarate + NH3
-
5997
shark
-
4.3.1.1
L-aspartate
natural substrate
5997
Gallus gallus
fumarate + NH3
-
5997
Gallus gallus
-
4.3.1.1
L-aspartate
natural substrate
5997
Escherichia coli
fumarate + NH3
-
5997
Escherichia coli
-
4.3.1.1
L-aspartate
natural substrate
5997
Bacterium cadaveris
fumarate + NH3
-
5997
Bacterium cadaveris
-
4.3.1.1
L-aspartate
natural substrate
5997
Frog
fumarate + NH3
-
5997
Frog
-
4.3.1.1
L-aspartate
natural substrate
5997
Anas platyrhynchos
fumarate + NH3
-
5997
Anas platyrhynchos
-
4.3.1.1
L-aspartate
natural substrate
5997
Yersinia pestis
fumarate + NH3
-
5997
Yersinia pestis
-
4.3.1.1
L-aspartate
natural substrate
5997
shark
fumarate + NH3
-
5997
shark
-
4.3.1.2
erythro-3-methylaspartate
-
5997
Bacterium cadaveris
?
-
-
-
-
4.3.1.2
erythro-3-methylaspartate
erythro-3-methyl-L-aspartate
5997
Clostridium tetanomorphum
?
-
-
-
-
4.3.1.2
erythro-3-methylaspartate
erythro-3-methyl-L-aspartate
5997
Clostridium tetanomorphum H1 / ATCC 15920
?
-
-
-
-
4.3.1.2
L-threo-3-methylaspartate
r
5997
Bacterium cadaveris
mesaconate + NH3
-
5997
Bacterium cadaveris
-
4.3.1.2
L-threo-3-methylaspartate
r
5997
Clostridium tetanomorphum
mesaconate + NH3
-
5997
Clostridium tetanomorphum
-
4.3.1.2
L-threo-3-methylaspartate
r
5997
Clostridium tetanomorphum H1 / ATCC 15920
mesaconate + NH3
-
5997
Clostridium tetanomorphum H1 / ATCC 15920
-
4.3.1.2
L-threo-3-methylaspartate
-
5997
Bacterium cadaveris
?
-
-
-
-
4.3.1.2
L-threo-3-methylaspartate
-
5997
Clostridium tetanomorphum
?
-
-
-
-
4.3.1.2
L-threo-3-methylaspartate
-
5997
Clostridium tetanomorphum H1 / ATCC 15920
?
-
-
-
-
4.3.1.3
L-histidine
natural substrate
5997
Salmonella enterica subsp. enterica serovar Typhimurium
urocanate + NH3
-
5997
Salmonella enterica subsp. enterica serovar Typhimurium
?
4.3.1.3
L-histidine
natural substrate
5997
Bacillus subtilis
urocanate + NH3
-
5997
Bacillus subtilis
?
4.3.1.3
L-histidine
natural substrate
5997
Homo sapiens
urocanate + NH3
-
5997
Homo sapiens
?
4.3.1.3
L-histidine
natural substrate
5997
Rattus norvegicus
urocanate + NH3
-
5997
Rattus norvegicus
?
4.3.1.3
L-histidine
natural substrate
5997
Pseudomonas fluorescens
urocanate + NH3
-
5997
Pseudomonas fluorescens
?
4.3.1.3
L-histidine
natural substrate
5997
Klebsiella aerogenes
urocanate + NH3
-
5997
Klebsiella aerogenes
?
4.3.1.3
L-histidine
natural substrate
5997
Pseudomonas aeruginosa
urocanate + NH3
-
5997
Pseudomonas aeruginosa
?
4.3.1.3
L-histidine
natural substrate
5997
Pseudomonas sp.
urocanate + NH3
-
5997
Pseudomonas sp.
?
4.3.1.3
L-histidine
natural substrate
5997
Comamonas testosteroni
urocanate + NH3
-
5997
Comamonas testosteroni
?
4.3.1.3
L-histidine
natural substrate
5997
toad
urocanate + NH3
-
5997
toad
?
4.3.1.3
L-histidine
natural substrate
5997
Pseudomonas fluorescens A.3.12
urocanate + NH3
-
5997
Pseudomonas fluorescens A.3.12
?
4.3.1.3
additional information
-
5997
Rattus norvegicus
?
-
-
-
-
4.3.1.3
additional information
-
5997
Pseudomonas fluorescens
?
-
-
-
-
4.3.1.3
additional information
deficiency of enzyme results in histidinemia
5997
Homo sapiens
?
-
-
-
-
4.3.1.3
additional information
-
5997
Pseudomonas fluorescens A.3.12
?
-
-
-
-
Subunits
EC Number
Subunits
Commentary
Organism
4.3.1.3
tetramer
-
Bacillus subtilis
4.3.1.3
tetramer
4 * 54000, sedimentation in the presence of 6 M guanidine hypochloride and mercaptoethanol
Comamonas testosteroni
4.3.1.3
tetramer
-
Pseudomonas sp.
General Stability (protein specific)
EC Number
General Stability
Organism
4.3.1.1
irreversible inactivation during prolonged centrifugation
Bacterium cadaveris
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
4.3.1.2
p-mercuribenzoate
-
Clostridium tetanomorphum
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
4.3.1.3
additional information
-
additional information
-
Pseudomonas fluorescens
4.3.1.3
additional information
-
additional information
-
Pseudomonas sp.
4.3.1.3
additional information
-
additional information
-
Rattus norvegicus
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
4.3.1.1
180000
-
sucrose density gradient centrifugation
Bacterium cadaveris
4.3.1.2
100000
-
sedimentation equilibrium
Clostridium tetanomorphum
4.3.1.3
54000
-
4 * 54000, sedimentation in the presence of 6 M guanidine hypochloride and mercaptoethanol
Comamonas testosteroni
4.3.1.3
189000
-
sucrose density gradient centrifugation
Pseudomonas fluorescens
4.3.1.3
200000
-
sedimentation equilibrium
Comamonas testosteroni
4.3.1.3
204000
-
sucrose density gradient centrifugation
toad
4.3.1.3
210000
-
sucrose density gradient centrifugation
Pseudomonas aeruginosa
4.3.1.3
210000
-
-
Pseudomonas sp.
4.3.1.3
218000
-
sucrose density gradient centrifugation
Pseudomonas fluorescens
4.3.1.3
220000
-
sucrose density gradient centrifugation
Bacillus subtilis
4.3.1.3
226000
-
sucrose density gradient centrifugation
Rattus norvegicus
4.3.1.3
243000
-
sucrose density gradient centrifugation
Homo sapiens
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
4.3.1.2
L-threo-3-methylaspartate
Bacterium cadaveris
-
?
-
-
-
4.3.1.2
L-threo-3-methylaspartate
Clostridium tetanomorphum
-
?
-
-
-
4.3.1.2
L-threo-3-methylaspartate
Clostridium tetanomorphum H1 / ATCC 15920
-
?
-
-
-
4.3.1.3
additional information
Rattus norvegicus
-
?
-
-
-
4.3.1.3
additional information
Pseudomonas fluorescens
-
?
-
-
-
4.3.1.3
additional information
Homo sapiens
deficiency of enzyme results in histidinemia
?
-
-
-
4.3.1.3
additional information
Pseudomonas fluorescens A.3.12
-
?
-
-
-
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
4.3.1.1
liver
-
Anas platyrhynchos
-
4.3.1.1
liver
-
Frog
-
4.3.1.1
liver
-
Gallus gallus
-
4.3.1.1
liver
-
shark
-
4.3.1.3
liver
-
Homo sapiens
-
4.3.1.3
liver
-
toad
-
4.3.1.3
liver
-
Rattus norvegicus
-
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
4.3.1.3
additional information
-
-
Bacillus subtilis
4.3.1.3
additional information
-
-
Pseudomonas fluorescens
4.3.1.3
additional information
-
-
Pseudomonas sp.
4.3.1.3
additional information
-
-
Rattus norvegicus
4.3.1.3
3.4
-
37°C, pH 9.0, 3.3 mM L-histidine
Rattus norvegicus
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4.3.1.1
fumarate + hydroxylamine
-
5997
Escherichia coli
?
-
-
-
-
4.3.1.1
fumarate + hydroxylamine
-
5997
Bacterium cadaveris
?
-
-
-
-
4.3.1.1
L-aspartate
r
5997
Gallus gallus
fumarate + NH3
-
5997
Gallus gallus
-
4.3.1.1
L-aspartate
r
5997
Escherichia coli
fumarate + NH3
-
5997
Escherichia coli
-
4.3.1.1
L-aspartate
r
5997
Frog
fumarate + NH3
-
5997
Frog
-
4.3.1.1
L-aspartate
r
5997
Anas platyrhynchos
fumarate + NH3
-
5997
Anas platyrhynchos
-
4.3.1.1
L-aspartate
r
5997
Yersinia pestis
fumarate + NH3
-
5997
Yersinia pestis
-
4.3.1.1
L-aspartate
r
5997
shark
fumarate + NH3
-
5997
shark
-
4.3.1.1
L-aspartate
natural substrate
5997
Gallus gallus
fumarate + NH3
-
5997
Gallus gallus
-
4.3.1.1
L-aspartate
natural substrate
5997
Escherichia coli
fumarate + NH3
-
5997
Escherichia coli
-
4.3.1.1
L-aspartate
natural substrate
5997
Bacterium cadaveris
fumarate + NH3
-
5997
Bacterium cadaveris
-
4.3.1.1
L-aspartate
natural substrate
5997
Frog
fumarate + NH3
-
5997
Frog
-
4.3.1.1
L-aspartate
natural substrate
5997
Anas platyrhynchos
fumarate + NH3
-
5997
Anas platyrhynchos
-
4.3.1.1
L-aspartate
natural substrate
5997
Yersinia pestis
fumarate + NH3
-
5997
Yersinia pestis
-
4.3.1.1
L-aspartate
natural substrate
5997
shark
fumarate + NH3
-
5997
shark
-
4.3.1.2
erythro-3-methylaspartate
-
5997
Bacterium cadaveris
?
-
-
-
-
4.3.1.2
erythro-3-methylaspartate
erythro-3-methyl-L-aspartate
5997
Clostridium tetanomorphum
?
-
-
-
-
4.3.1.2
erythro-3-methylaspartate
erythro-3-methyl-L-aspartate
5997
Clostridium tetanomorphum H1 / ATCC 15920
?
-
-
-
-
4.3.1.2
L-threo-3-methylaspartate
r
5997
Bacterium cadaveris
mesaconate + NH3
-
5997
Bacterium cadaveris
-
4.3.1.2
L-threo-3-methylaspartate
r
5997
Clostridium tetanomorphum
mesaconate + NH3
-
5997
Clostridium tetanomorphum
-
4.3.1.2
L-threo-3-methylaspartate
r
5997
Clostridium tetanomorphum H1 / ATCC 15920
mesaconate + NH3
-
5997
Clostridium tetanomorphum H1 / ATCC 15920
-
4.3.1.2
L-threo-3-methylaspartate
-
5997
Bacterium cadaveris
?
-
-
-
-
4.3.1.2
L-threo-3-methylaspartate
-
5997
Clostridium tetanomorphum
?
-
-
-
-
4.3.1.2
L-threo-3-methylaspartate
-
5997
Clostridium tetanomorphum H1 / ATCC 15920
?
-
-
-
-
4.3.1.3
L-histidine
natural substrate
5997
Salmonella enterica subsp. enterica serovar Typhimurium
urocanate + NH3
-
5997
Salmonella enterica subsp. enterica serovar Typhimurium
?
4.3.1.3
L-histidine
natural substrate
5997
Bacillus subtilis
urocanate + NH3
-
5997
Bacillus subtilis
?
4.3.1.3
L-histidine
natural substrate
5997
Homo sapiens
urocanate + NH3
-
5997
Homo sapiens
?
4.3.1.3
L-histidine
natural substrate
5997
Rattus norvegicus
urocanate + NH3
-
5997
Rattus norvegicus
?
4.3.1.3
L-histidine
natural substrate
5997
Pseudomonas fluorescens
urocanate + NH3
-
5997
Pseudomonas fluorescens
?
4.3.1.3
L-histidine
natural substrate
5997
Klebsiella aerogenes
urocanate + NH3
-
5997
Klebsiella aerogenes
?
4.3.1.3
L-histidine
natural substrate
5997
Pseudomonas aeruginosa
urocanate + NH3
-
5997
Pseudomonas aeruginosa
?
4.3.1.3
L-histidine
natural substrate
5997
Pseudomonas sp.
urocanate + NH3
-
5997
Pseudomonas sp.
?
4.3.1.3
L-histidine
natural substrate
5997
Comamonas testosteroni
urocanate + NH3
-
5997
Comamonas testosteroni
?
4.3.1.3
L-histidine
natural substrate
5997
toad
urocanate + NH3
-
5997
toad
?
4.3.1.3
L-histidine
natural substrate
5997
Pseudomonas fluorescens A.3.12
urocanate + NH3
-
5997
Pseudomonas fluorescens A.3.12
?
4.3.1.3
additional information
-
5997
Rattus norvegicus
?
-
-
-
-
4.3.1.3
additional information
-
5997
Pseudomonas fluorescens
?
-
-
-
-
4.3.1.3
additional information
deficiency of enzyme results in histidinemia
5997
Homo sapiens
?
-
-
-
-
4.3.1.3
additional information
-
5997
Pseudomonas fluorescens A.3.12
?
-
-
-
-
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
4.3.1.3
tetramer
-
Bacillus subtilis
4.3.1.3
tetramer
4 * 54000, sedimentation in the presence of 6 M guanidine hypochloride and mercaptoethanol
Comamonas testosteroni
4.3.1.3
tetramer
-
Pseudomonas sp.