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Literature summary extracted from
- Sequential degradation of heparin in Flavobacterium heparinum. Purification and properties of five enzymes involved in heparin degradation (1973), J. Biol. Chem., 248, 6408-6415.
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 3.10.1.1 | cytoplasm | - |
Pedobacter heparinus | 5737 | - |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.1.6.11 | Pedobacter heparinus | - |
- |
- |
| 3.2.1.56 | Pedobacter heparinus | - |
- |
- |
| 3.10.1.1 | Pedobacter heparinus | - |
- |
- |
| 4.2.2.7 | Pedobacter heparinus | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.1.6.11 | partial | Pedobacter heparinus |
| 3.10.1.1 | - |
Pedobacter heparinus |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.56 | additional information | - |
6.470 mg/h | Pedobacter heparinus |
| 3.2.1.56 | 259 | - |
crude, enzymatic activity measured by the disappearance of substrate | Pedobacter heparinus |
| 4.2.2.7 | additional information | - |
8.855 mg product/mg protein/h | Pedobacter heparinus |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.1.6.11 | glucosamine-2,6-disulfate + H2O | substrate yielded from enzymatic degradation of heparin | Pedobacter heparinus | glucosamine-6-sulfate + SO42- | - |
? |  |
| 3.1.6.11 | glucosamine-N-sulfate + H2O | - |
Pedobacter heparinus | glucosamine + SO42- | - |
? | |
| 3.2.1.56 | disaccharide + SO42- | overview disulfated disaccharides | Pedobacter heparinus | glucosamine 2,6-disulfate + uronic acid | alpha, beta-keto acid | ? | |
| 3.2.1.56 | glucosamine 2,6-disulfate | - |
Pedobacter heparinus | glucosamine-N-sulfate + SO42- | - |
? | |
| 3.2.1.56 | heparin + H2O | - |
Pedobacter heparinus | trisulfated heparin disaccharide + disulfated heparin disaccharide + D-glucosamine 2,6-disulfate + D-glucosamine-N-sulfate + SO42- | - |
? | |
pH Stability
| EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.10.1.1 | 5 | 7.4 | stable between | Pedobacter heparinus |
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Release 2023.1 (January 2023)
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