EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
6.2.1.12 | 50000 | - |
gel filtration | Centaurium erythraea |
6.2.1.27 | 50000 | - |
gel filtration | Centaurium erythraea |
6.2.1.37 | 50000 | - |
gel filtration | Centaurium erythraea |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
6.2.1.12 | ATP + 4-coumarate + CoA | Centaurium erythraea | 4-coumarate-CoA ligase is the last enzyme of the general phenylpropanoid metabolism, supplying substrates for lignin formation and other individual phenylpropanoid pathways | ? | - |
? | |
6.2.1.37 | ATP + 3-hydroxybenzoate + CoA | Centaurium erythraea | the enzyme is involved in xanthone biosynthesis | AMP + diphosphate + 3-hydroxybenzoyl-CoA | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
6.2.1.12 | Centaurium erythraea | - |
- |
- |
6.2.1.27 | Centaurium erythraea | - |
- |
- |
6.2.1.37 | Centaurium erythraea | - |
- |
- |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
6.2.1.27 | cell suspension culture | in the course of culture growth, the activity increases from day 6 and reaches maximum at day 10. This correlates well with the increases in the cell fresh weight and the xanthone content that starts also at day 6 | Centaurium erythraea | - |
6.2.1.37 | cell suspension culture | in the course of culture growth, the activities of both enzymes increase from day 6 and reached maxima at day 10. This correlates well with the increases in the cell fresh weight and the xanthone content that starts also at day 6 | Centaurium erythraea | - |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
6.2.1.12 | ATP + 4-coumarate + CoA | - |
Centaurium erythraea | AMP + diphosphate + 4-coumaroyl-CoA | - |
? | |
6.2.1.12 | ATP + 4-coumarate + CoA | 4-coumarate-CoA ligase is the last enzyme of the general phenylpropanoid metabolism, supplying substrates for lignin formation and other individual phenylpropanoid pathways | Centaurium erythraea | ? | - |
? | |
6.2.1.12 | ATP + caffeate + CoA | at 4% of the activity relative to 4-coumarate | Centaurium erythraea | AMP + diphosphate + caffeoyl-CoA | - |
? | |
6.2.1.12 | ATP + ferulate + CoA | 3% of the activity relative to 4-coumarate | Centaurium erythraea | AMP + diphosphate + feruloyl-CoA | - |
? | |
6.2.1.27 | ATP + 4-hydroxybenzoate + CoA | the bifunctional enzyme is also active with 3-hydroxybenzoate (cf. EC 6.2.1.37). Activity with 4-hydroxybenzoate is 17% of the activity with 3-hydroxybenzoate | Centaurium erythraea | AMP + diphosphate + 4-hydroxybenzoyl-CoA | - |
? | |
6.2.1.27 | additional information | no activity with 2-hydroxybenzoate, 2,3-dihydroxybenzoate, 3,4-dihydroxybenzoate, 3,5-dihydroxybenzoate and cinnamic acid | Centaurium erythraea | ? | - |
? | |
6.2.1.37 | ATP + 3-hydroxybenzoate + CoA | the enzyme is involved in xanthone biosynthesis | Centaurium erythraea | AMP + diphosphate + 3-hydroxybenzoyl-CoA | - |
? | |
6.2.1.37 | ATP + 3-hydroxybenzoate + CoA | the bifunctional enzyme is also active with 4-hydroxybenzoate (cf. EC 6.2.1.27). Activity with 4-hydroxybenzoate is 17% of the activity with 3-hydroxybenzoate | Centaurium erythraea | AMP + diphosphate + 3-hydroxybenzoyl-CoA | - |
? | |
6.2.1.37 | additional information | no activity with 2-hydroxybenzoate, 2,3-dihydroxybenzoate, 3,4-dihydroxybenzoate, 3,5-dihydroxybenzoate and cinnamic acid | Centaurium erythraea | ? | - |
? |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
6.2.1.12 | 30 | - |
- |
Centaurium erythraea |
6.2.1.27 | 20 | 30 | - |
Centaurium erythraea |
6.2.1.37 | 20 | 30 | - |
Centaurium erythraea |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
6.2.1.12 | 6.5 | - |
- |
Centaurium erythraea |
6.2.1.27 | 7 | - |
- |
Centaurium erythraea |
6.2.1.37 | 7 | - |
- |
Centaurium erythraea |