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Literature summary extracted from

  • K๖ster, S.; Stier, G.; Ficner, R.; Hoezer, M.; Curtius, H.C.; Suck, D.; Ghisla, S.
    Location of the active site and proposed catalytic mechanism of pterin-4alpha-carbinolamine dehydratase (1996), Eur. J. Biochem., 241, 858-864.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.2.1.96 wild-type and mutant enzymes Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
4.2.1.96 E57A the His79Ala mutant and the His79Ser mutant exhibit about 40% the activity of the wild-type enzyme. In the mutant enzymes His61Ala and His62Ala the activity is reduced to 10%. In the mutant enzymes Asp60Ala and Arg87Ala the activity is reduced to 30%. The Glu57Ala mutant and the His61Ala,His62Ala double mutant show no activity Homo sapiens
4.2.1.96 H61A the His79Ala mutant and the His79Ser mutant exhibit about 40% the activity of the wild-type enzyme. In the mutant enzymes His61Ala and His62Ala the activity is reduced to 10%. In the mutant enzymes Asp60Ala and Arg87Ala the activity is reduced to 30%. The Glu57Ala mutant and the His61Ala,His62Ala double mutant show no activity Homo sapiens
4.2.1.96 H61A/H62A the His79Ala mutant and the His79Ser mutant exhibit about 40% the activity of the wild-type enzyme. In the mutant enzymes His61Ala and His62Ala the activity is reduced to 10%. In the mutant enzymes Asp60Ala and Arg87Ala the activity is reduced to 30%. The Glu57Ala mutant and the His61Ala,His62Ala double mutant show no activity Homo sapiens
4.2.1.96 H62A the His79Ala mutant and the His79Ser mutant exhibit about 40% the activity of the wild-type enzyme. In the mutant enzymes His61Ala and His62Ala the activity is reduced to 10%. In the mutant enzymes Asp60Ala and Arg87Ala the activity is reduced to 30%. The Glu57Ala mutant and the His61Ala,His62Ala double mutant show no activity Homo sapiens
4.2.1.96 H79A the His79Ala mutant and the His79Ser mutant exhibit about 40% the activity of the wild-type enzyme. In the mutant enzymes His61Ala and His62Ala the activity is reduced to 10%. In the mutant enzymes Asp60Ala and Arg87Ala the activity is reduced to 30%. The Glu57Ala mutant and the His61Ala,His62Ala double mutant show no activity Homo sapiens
4.2.1.96 H79S the His79Ala mutant and the His79Ser mutant exhibit about 40% the activity of the wild-type enzyme. In the mutant enzymes His61Ala and His62Ala the activity is reduced to 10%. In the mutant enzymes Asp60Ala and Arg87Ala the activity is reduced to 30%. The Glu57Ala mutant and the His61Ala,His62Ala double mutant show no activity Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.2.1.96 0.007
-
 6(S)-methyl-7,8-dihydropterin-4a-carbinolamine mutant enzyme Tyr69Thr Homo sapiens
4.2.1.96 0.008
-
 6(S)-methyl-7,8-dihydropterin-4a-carbinolamine mutant enzyme Y69F Homo sapiens
4.2.1.96 0.01
-
 6(S)-methyl-7,8-dihydropterin-4a-carbinolamine mutant enzyme H79A Homo sapiens
4.2.1.96 0.012
-
 6(S)-methyl-7,8-dihydropterin-4a-carbinolamine mutant enzyme His79Ser Homo sapiens
4.2.1.96 0.017
-
 6(S)-methyl-7,8-dihydropterin-4a-carbinolamine mutant enzyme Cys81Arg Homo sapiens
4.2.1.96 0.018
-
 6(S)-methyl-7,8-dihydropterin-4a-carbinolamine mutant enzyme D60A Homo sapiens
4.2.1.96 0.02
-
 6(S)-methyl-7,8-dihydropterin-4a-carbinolamine mutant enzyme Arg87Ala Homo sapiens
4.2.1.96 0.025
-
 6(S)-methyl-7,8-dihydropterin-4a-carbinolamine wild type enzyme Homo sapiens
4.2.1.96 0.04
-
 6(S)-methyl-7,8-dihydropterin-4a-carbinolamine mutant enzyme Cys81Ser Homo sapiens
4.2.1.96 0.075
-
 6(S)-methyl-7,8-dihydropterin-4a-carbinolamine mutant enzyme H62A Homo sapiens
4.2.1.96 2.2
-
 6(S)-methyl-7,8-dihydropterin-4a-carbinolamine mutant enzyme H61A Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
4.2.1.96 Homo sapiens
-
-
-
4.2.1.96 Rattus norvegicus
-
-
-

Reaction

EC Number Reaction Comment Organism Reaction ID
4.2.1.96 4a-hydroxytetrahydrobiopterin = 6,7-dihydrobiopterin + H2O the mechanism of dehydration involves base catalysis at the N(5)-H group of the substrate by His61 Homo sapiens
a

Source Tissue

EC Number Source Tissue Comment Organism Textmining
4.2.1.96 liver
-
 Homo sapiens
-
4.2.1.96 liver
-
 Rattus norvegicus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.1.96 6(S)-methyl-7,8-dihydropterin-4a-carbinolamine
-
 Homo sapiens ?
-
 ?