Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Stamford, N.P.J.; Capretta, A.; Battersby, A.R.
    Expression, purification and characterisation of the product from the Bacillus subtilis hemD gene, uroporphyrinogen III synthase (1995), Eur. J. Biochem., 231, 236-241.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.2.1.75 expression in Escherichia coli Bacillus subtilis

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.2.1.75 Butane-2,3-dione
-
 Bacillus subtilis
4.2.1.75 diethyl dicarbonate
-
 Bacillus subtilis
4.2.1.75 NaN3 60% residual activity with 5.0 mM Bacillus subtilis
4.2.1.75 Phenylglyoxal
-
 Bacillus subtilis
4.2.1.75 pyridoxal 5'-phosphate Pyridoxal 5'-phosphate and subsequent treatment with NaBH4 Bacillus subtilis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.2.1.75 0.00033
-
 Hydroxymethylbilane
-
 Bacillus subtilis

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.2.1.75 additional information bivalent ions, such as MgCl2 up to 20 mM, fail to stimulate enzyme activity Bacillus subtilis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.2.1.75 29100
-
 nucleotide sequence Bacillus subtilis
4.2.1.75 32000
-
 SDS-PAGE Bacillus subtilis
4.2.1.75 41500
-
 gel filtration Bacillus subtilis

Organism

EC Number Organism UniProt Comment Textmining
4.2.1.75 Bacillus subtilis
-
-
-

Oxidation Stability

EC Number Oxidation Stability Organism
4.2.1.75 photooxidation in presence of Rose Bengal Bacillus subtilis

Purification (Commentary)

EC Number Purification (Comment) Organism
4.2.1.75 expression in Escherichia coli Bacillus subtilis

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.2.1.75 9.4
-
 hydroxymethylbilane Bacillus subtilis

Storage Stability

EC Number Storage Stability Organism
4.2.1.75 -70¦C, at least two years Bacillus subtilis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.1.75 Hydroxymethylbilane
-
 Bacillus subtilis Uroporphyrinogen III
-
 ?

Subunits

EC Number Subunits Comment Organism
4.2.1.75 monomer SDS-PAGE, gel filtration, nucleotide sequence Bacillus subtilis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.2.1.75 45
-
-
 Bacillus subtilis

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
4.2.1.75 additional information
-
 more thermostable than enzymes from other sources Bacillus subtilis
4.2.1.75 30
-
 retention of 100% activity after 24 h Bacillus subtilis
4.2.1.75 45
-
 retention of 90% activity after 24 h Bacillus subtilis
4.2.1.75 60
-
 completely inactivated in 1 min Bacillus subtilis

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.2.1.75 4.6
-
 Hydroxymethylbilane
-
 Bacillus subtilis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.2.1.75 8.3
-
-
 Bacillus subtilis