EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
4.2.1.75 | additional information | no bound cofactor | Euglena gracilis |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
4.2.1.75 | Butane-2,3-dione | 20% residual activity after 45 min at 40 mM | Euglena gracilis | |
4.2.1.75 | diethyl dicarbonate | 10% residual activity after 5 min at 0.41 mM | Euglena gracilis | |
4.2.1.75 | NaN3 | 25-30% residual activity with 1.0 mM | Euglena gracilis | |
4.2.1.75 | Phenylglyoxal | 50% residual activity after 1 min at 0.3 mM | Euglena gracilis | |
4.2.1.75 | pyridoxal 5'-phosphate | - |
Euglena gracilis | |
4.2.1.75 | uroporphyrinogen III | product inhibition | Euglena gracilis |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
4.2.1.75 | 0.012 | 0.04 | Hydroxymethylbilane | - |
Euglena gracilis |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
4.2.1.75 | additional information | no metal ions present in enzyme | Euglena gracilis |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
4.2.1.75 | 31000 | - |
SDS-PAGE | Euglena gracilis |
4.2.1.75 | 38500 | - |
SDS-PAGE, gel filtration | Euglena gracilis |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
4.2.1.75 | Hydroxymethylbilane | Euglena gracilis | synthesis of uroporphyrinogen III, key intermediate for biosynthesis of tetrapyrrolic compounds like chlorophylls, hemes, cytochromes and vitamin B12 | ? | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
4.2.1.75 | Euglena gracilis | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
4.2.1.75 | - |
Euglena gracilis |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
4.2.1.75 | additional information | - |
- |
Euglena gracilis |
EC Number | Storage Stability | Organism |
---|---|---|
4.2.1.75 | -70¦C, several weeks | Euglena gracilis |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
4.2.1.75 | Hydroxymethylbilane | - |
Euglena gracilis | Uroporphyrinogen III | - |
? | |
4.2.1.75 | Hydroxymethylbilane | synthesis of uroporphyrinogen III, key intermediate for biosynthesis of tetrapyrrolic compounds like chlorophylls, hemes, cytochromes and vitamin B12 | Euglena gracilis | ? | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
4.2.1.75 | monomer | SDS-PAGE, gel filtration | Euglena gracilis |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
4.2.1.75 | 25 | - |
enzyme assay at | Euglena gracilis |