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Literature summary extracted from

  • Malila, L.H.; Siivari, K.M.; Mäkelä, M.J.; Jalonen, J.E.; Latipää, P.M.; Kunau, W.H.; Hiltunen, J.K.
    Enzymes converting D-3-hydroxyacyl-CoA to trans-2-enoyl-CoA. Microsomal and peroxisomal isoenzymes in rat liver (1993), J. Biol. Chem., 268, 21578-21585.
    View publication on PubMed

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
4.2.1.119 microsome enzyme different from peroxisomal hydratase 2 though their kinetic properties are similar. They differ in immunological data, subunit size, and chromatographic behaviour, the peroxisomal isoform is not recognized by the antibody to its microsomal counterpart Rattus norvegicus
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4.2.1.119 peroxisome enzyme different from microsomal hydratase 2 though their kinetic properties are similar. They differ in immunological data, subunit size, and chromatographic behaviour, the peroxisomal isoform is not recognized by the antibody to its microsomal counterpart Rattus norvegicus 5777
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Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.2.1.119 31500
-
 2 * 31500, microsomal isoform, SDS-PAGE Rattus norvegicus
4.2.1.119 33500
-
 2 * 33500, peroxisomal isoform, SDS-PAGE Rattus norvegicus
4.2.1.119 60000
-
 microsomal isoform, gel filtration Rattus norvegicus
4.2.1.119 62000
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 peroxisomal isoform, gel filtration Rattus norvegicus

Organism

EC Number Organism UniProt Comment Textmining
4.2.1.119 Rattus norvegicus
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-
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Purification (Commentary)

EC Number Purification (Comment) Organism
4.2.1.119 0.2% yield,the microsomal isoform elutes at 0.4-0.7 MKCl, at low salt concentrations, whereas the peroxisomal isoform elutes at high salt concentrations Rattus norvegicus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
4.2.1.119 liver
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 Rattus norvegicus
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Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.2.1.119 37.5
-
 purified enzyme Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.1.119 (R)-3-hydroxydecanoyl-CoA
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 Rattus norvegicus trans-2-decenoyl-CoA + H2O
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 r
4.2.1.119 additional information the (S)-3-hydroxy-CoA is not dehydrated Rattus norvegicus ?
-
 ?

Subunits

EC Number Subunits Comment Organism
4.2.1.119 homodimer 2 * 31500, microsomal isoform, SDS-PAGE Rattus norvegicus
4.2.1.119 homodimer 2 * 33500, peroxisomal isoform, SDS-PAGE Rattus norvegicus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.2.1.119 25
-
 assay at Rattus norvegicus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.2.1.119 9
-
 assay at Rattus norvegicus