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Literature summary extracted from

  • Nagasawa, T.; Kanzaki, H.; Yamada, H.
    Cystathionine gamma-lyase of Streptomyces phaeochromogenes. The occurrence of cystathionine gamma-lyase in filamentous bacteria and its purification and characterization (1984), J. Biol. Chem., 259, 10393-10403.
    View publication on PubMed

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
4.4.1.1
-
Streptomyces phaeochromogenes

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.4.1.1 AgNO3 alpha,beta-elimination L-homoserine Streptomyces phaeochromogenes
4.4.1.1 beta-cyano-L-Ala alpha,beta-elimination L-homoserine Streptomyces phaeochromogenes
4.4.1.1 cycloserine
-
Streptomyces phaeochromogenes
4.4.1.1 Hg2+ HgCl2, alpha,beta-elimination L-homoserine Streptomyces phaeochromogenes
4.4.1.1 hydroxylamine alpha,beta-elimination of L-homoserine Streptomyces phaeochromogenes
4.4.1.1 PCMB alpha,beta-elimination L-homoserine Streptomyces phaeochromogenes
4.4.1.1 Penicillamine alpha,beta-elimination of L-homoserine; D-penicillamine; L-penicillamine Streptomyces phaeochromogenes
4.4.1.1 phenylhydrazine alpha,beta-elimination L-homoserine as substrate Streptomyces phaeochromogenes
4.4.1.1 propargylglycine alpha,beta-elimination L-homoserine Streptomyces phaeochromogenes
4.4.1.1 Semicarbazide alpha,beta-elimination L-homoserine as substrate Streptomyces phaeochromogenes
4.4.1.1 Sodium cyanide alpha,beta-elimination, 1 mM, 82% inhibition Streptomyces phaeochromogenes
4.4.1.1 Zn2+ ZnCl2, alpha,beta-elimination L-homoserine Streptomyces phaeochromogenes

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.4.1.1 0.204
-
L-cystathionine
-
Streptomyces phaeochromogenes
4.4.1.1 0.209
-
DL-lanthionine
-
Streptomyces phaeochromogenes
4.4.1.1 0.909
-
L-cystine
-
Streptomyces phaeochromogenes
4.4.1.1 2.39
-
beta-chloro-L-Ala
-
Streptomyces phaeochromogenes
4.4.1.1 5.18
-
DL-vinylglycine
-
Streptomyces phaeochromogenes
4.4.1.1 12.5
-
L-homoserine
-
Streptomyces phaeochromogenes

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.4.1.1 41000
-
4 * 41000, SDS-PAGE Streptomyces phaeochromogenes
4.4.1.1 162000
-
gel filtration Streptomyces phaeochromogenes
4.4.1.1 168000
-
equilibrium sedimentation Streptomyces phaeochromogenes

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.48 Streptomyces phaeochromogenes
-
-
-
4.4.1.1 Amycolatopsis lactamdurans
-
-
-
4.4.1.1 Gordonia rubripertincta
-
-
-
4.4.1.1 Micromonospora chalcea
-
-
-
4.4.1.1 Micromonospora coerulea
-
-
-
4.4.1.1 Mycolicibacterium phlei
-
-
-
4.4.1.1 Mycolicibacterium smegmatis
-
-
-
4.4.1.1 Nocardia asteroides
-
-
-
4.4.1.1 Nocardia erythropolis
-
-
-
4.4.1.1 Nocardia globerula
-
-
-
4.4.1.1 Nonomuraea angiospora
-
-
-
4.4.1.1 Streptomyces lydicus
-
-
-
4.4.1.1 Streptomyces microflavus
-
-
-
4.4.1.1 Streptomyces netropsis
-
-
-
4.4.1.1 Streptomyces olivochromogenes
-
-
-
4.4.1.1 Streptomyces phaeochromogenes
-
IFO 3105
-
4.4.1.1 Streptomyces rimosus
-
-
-
4.4.1.1 Streptomyces virginiae
-
-
-
4.4.1.1 Streptosporangium roseum
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.4.1.1
-
Streptomyces phaeochromogenes

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.4.1.1 2.6
-
-
Streptomyces phaeochromogenes

Storage Stability

EC Number Storage Stability Organism
4.4.1.1 -20°C, in 45% glycerol solution, containing 10 mM potassium phosphate, pH 7.5, 0.02 mM pyridoxal 5'-phosphate, 0.12 mM EDTA, 0.2 mM DTT, stable for 2 months or more Streptomyces phaeochromogenes
4.4.1.1 4°C, 10 mM potassium phosphate, pH 7.5, containing 0.02 mM pyridoxal phosphate, 0.12 mM EDTA, 0.2 mM dithiothreitol stable for 2 weeks Streptomyces phaeochromogenes

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.48 O-acetyl-L-homoserine + L-cysteine
-
Streptomyces phaeochromogenes L-cystathionine + acetate
-
?
4.4.1.1 beta-chloro-L-Ala alpha,beta-elimination Streptomyces phaeochromogenes ?
-
?
4.4.1.1 beta-chloro-L-Ala alpha,gamma-elimination Streptomyces phaeochromogenes ?
-
?
4.4.1.1 djenkolic acid alpha,beta-elimination Streptomyces phaeochromogenes ?
-
?
4.4.1.1 djenkolic acid alpha-gamma-elimination Streptomyces phaeochromogenes ?
-
?
4.4.1.1 DL-vinylglycine alpha,beta-elimination Streptomyces phaeochromogenes ?
-
?
4.4.1.1 DL-vinylglycine alpha,gamma-elimination Streptomyces phaeochromogenes ?
-
?
4.4.1.1 L-cystathionine + H2O alpha,gamma-elimination Streptomyces phaeochromogenes L-cysteine + 2-oxobutanoate + NH3
-
?
4.4.1.1 L-cystathionine + H2O alpha,beta-elimination Streptomyces phaeochromogenes L-homocysteine + pyruvate + NH3
-
?
4.4.1.1 L-cystine alpha,beta-elimination Streptomyces phaeochromogenes ?
-
?
4.4.1.1 L-cystine alpha,gamma-elimination Streptomyces phaeochromogenes ?
-
?
4.4.1.1 L-homoserine alpha,gamma-elimination Streptomyces phaeochromogenes ?
-
?
4.4.1.1 Lanthionine alpha,beta-elimination Streptomyces phaeochromogenes ?
-
?
4.4.1.1 Lanthionine alpha,gamma-elimination Streptomyces phaeochromogenes ?
-
?
4.4.1.1 Lanthionine DL-lanthionine Streptomyces phaeochromogenes ?
-
?
4.4.1.1 S-methyl-L-Cys alpha,beta-elimination Streptomyces phaeochromogenes ?
-
?

Subunits

EC Number Subunits Comment Organism
4.4.1.1 tetramer 4 * 41000, SDS-PAGE Streptomyces phaeochromogenes

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.4.1.1 50
-
-
Streptomyces phaeochromogenes

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
4.4.1.1 50
-
0.04 mM pyridoxal 5'-phosphate, 10 min, stable Streptomyces phaeochromogenes
4.4.1.1 55
-
0.04 mM pyridoxal 5'-phosphate, 10 min, 15% loss of activity Streptomyces phaeochromogenes
4.4.1.1 60
-
0.04 mM pyridoxal 5'-phosphate, 10 min, 50% loss of activity Streptomyces phaeochromogenes

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.4.1.1 9
-
-
Streptomyces phaeochromogenes

Cofactor

EC Number Cofactor Comment Organism Structure
4.4.1.1 pyridoxal 5'-phosphate contains 4 mol of pyridoxal 5'-phosphate per mol of enzyme Streptomyces phaeochromogenes