EC Number | Crystallization (Comment) | Organism |
---|---|---|
4.4.1.1 | - |
Streptomyces phaeochromogenes |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
4.4.1.1 | AgNO3 | alpha,beta-elimination L-homoserine | Streptomyces phaeochromogenes | |
4.4.1.1 | beta-cyano-L-Ala | alpha,beta-elimination L-homoserine | Streptomyces phaeochromogenes | |
4.4.1.1 | cycloserine | - |
Streptomyces phaeochromogenes | |
4.4.1.1 | Hg2+ | HgCl2, alpha,beta-elimination L-homoserine | Streptomyces phaeochromogenes | |
4.4.1.1 | hydroxylamine | alpha,beta-elimination of L-homoserine | Streptomyces phaeochromogenes | |
4.4.1.1 | PCMB | alpha,beta-elimination L-homoserine | Streptomyces phaeochromogenes | |
4.4.1.1 | Penicillamine | alpha,beta-elimination of L-homoserine; D-penicillamine; L-penicillamine | Streptomyces phaeochromogenes | |
4.4.1.1 | phenylhydrazine | alpha,beta-elimination L-homoserine as substrate | Streptomyces phaeochromogenes | |
4.4.1.1 | propargylglycine | alpha,beta-elimination L-homoserine | Streptomyces phaeochromogenes | |
4.4.1.1 | Semicarbazide | alpha,beta-elimination L-homoserine as substrate | Streptomyces phaeochromogenes | |
4.4.1.1 | Sodium cyanide | alpha,beta-elimination, 1 mM, 82% inhibition | Streptomyces phaeochromogenes | |
4.4.1.1 | Zn2+ | ZnCl2, alpha,beta-elimination L-homoserine | Streptomyces phaeochromogenes |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
4.4.1.1 | 0.204 | - |
L-cystathionine | - |
Streptomyces phaeochromogenes | |
4.4.1.1 | 0.209 | - |
DL-lanthionine | - |
Streptomyces phaeochromogenes | |
4.4.1.1 | 0.909 | - |
L-cystine | - |
Streptomyces phaeochromogenes | |
4.4.1.1 | 2.39 | - |
beta-chloro-L-Ala | - |
Streptomyces phaeochromogenes | |
4.4.1.1 | 5.18 | - |
DL-vinylglycine | - |
Streptomyces phaeochromogenes | |
4.4.1.1 | 12.5 | - |
L-homoserine | - |
Streptomyces phaeochromogenes |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
4.4.1.1 | 41000 | - |
4 * 41000, SDS-PAGE | Streptomyces phaeochromogenes |
4.4.1.1 | 162000 | - |
gel filtration | Streptomyces phaeochromogenes |
4.4.1.1 | 168000 | - |
equilibrium sedimentation | Streptomyces phaeochromogenes |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.5.1.48 | Streptomyces phaeochromogenes | - |
- |
- |
4.4.1.1 | Amycolatopsis lactamdurans | - |
- |
- |
4.4.1.1 | Gordonia rubripertincta | - |
- |
- |
4.4.1.1 | Micromonospora chalcea | - |
- |
- |
4.4.1.1 | Micromonospora coerulea | - |
- |
- |
4.4.1.1 | Mycolicibacterium phlei | - |
- |
- |
4.4.1.1 | Mycolicibacterium smegmatis | - |
- |
- |
4.4.1.1 | Nocardia asteroides | - |
- |
- |
4.4.1.1 | Nocardia erythropolis | - |
- |
- |
4.4.1.1 | Nocardia globerula | - |
- |
- |
4.4.1.1 | Nonomuraea angiospora | - |
- |
- |
4.4.1.1 | Streptomyces lydicus | - |
- |
- |
4.4.1.1 | Streptomyces microflavus | - |
- |
- |
4.4.1.1 | Streptomyces netropsis | - |
- |
- |
4.4.1.1 | Streptomyces olivochromogenes | - |
- |
- |
4.4.1.1 | Streptomyces phaeochromogenes | - |
IFO 3105 | - |
4.4.1.1 | Streptomyces rimosus | - |
- |
- |
4.4.1.1 | Streptomyces virginiae | - |
- |
- |
4.4.1.1 | Streptosporangium roseum | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
4.4.1.1 | - |
Streptomyces phaeochromogenes |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
4.4.1.1 | 2.6 | - |
- |
Streptomyces phaeochromogenes |
EC Number | Storage Stability | Organism |
---|---|---|
4.4.1.1 | -20°C, in 45% glycerol solution, containing 10 mM potassium phosphate, pH 7.5, 0.02 mM pyridoxal 5'-phosphate, 0.12 mM EDTA, 0.2 mM DTT, stable for 2 months or more | Streptomyces phaeochromogenes |
4.4.1.1 | 4°C, 10 mM potassium phosphate, pH 7.5, containing 0.02 mM pyridoxal phosphate, 0.12 mM EDTA, 0.2 mM dithiothreitol stable for 2 weeks | Streptomyces phaeochromogenes |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.5.1.48 | O-acetyl-L-homoserine + L-cysteine | - |
Streptomyces phaeochromogenes | L-cystathionine + acetate | - |
? | |
4.4.1.1 | beta-chloro-L-Ala | alpha,beta-elimination | Streptomyces phaeochromogenes | ? | - |
? | |
4.4.1.1 | beta-chloro-L-Ala | alpha,gamma-elimination | Streptomyces phaeochromogenes | ? | - |
? | |
4.4.1.1 | djenkolic acid | alpha,beta-elimination | Streptomyces phaeochromogenes | ? | - |
? | |
4.4.1.1 | djenkolic acid | alpha-gamma-elimination | Streptomyces phaeochromogenes | ? | - |
? | |
4.4.1.1 | DL-vinylglycine | alpha,beta-elimination | Streptomyces phaeochromogenes | ? | - |
? | |
4.4.1.1 | DL-vinylglycine | alpha,gamma-elimination | Streptomyces phaeochromogenes | ? | - |
? | |
4.4.1.1 | L-cystathionine + H2O | alpha,gamma-elimination | Streptomyces phaeochromogenes | L-cysteine + 2-oxobutanoate + NH3 | - |
? | |
4.4.1.1 | L-cystathionine + H2O | alpha,beta-elimination | Streptomyces phaeochromogenes | L-homocysteine + pyruvate + NH3 | - |
? | |
4.4.1.1 | L-cystine | alpha,beta-elimination | Streptomyces phaeochromogenes | ? | - |
? | |
4.4.1.1 | L-cystine | alpha,gamma-elimination | Streptomyces phaeochromogenes | ? | - |
? | |
4.4.1.1 | L-homoserine | alpha,gamma-elimination | Streptomyces phaeochromogenes | ? | - |
? | |
4.4.1.1 | Lanthionine | alpha,beta-elimination | Streptomyces phaeochromogenes | ? | - |
? | |
4.4.1.1 | Lanthionine | alpha,gamma-elimination | Streptomyces phaeochromogenes | ? | - |
? | |
4.4.1.1 | Lanthionine | DL-lanthionine | Streptomyces phaeochromogenes | ? | - |
? | |
4.4.1.1 | S-methyl-L-Cys | alpha,beta-elimination | Streptomyces phaeochromogenes | ? | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
4.4.1.1 | tetramer | 4 * 41000, SDS-PAGE | Streptomyces phaeochromogenes |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
4.4.1.1 | 50 | - |
- |
Streptomyces phaeochromogenes |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
4.4.1.1 | 50 | - |
0.04 mM pyridoxal 5'-phosphate, 10 min, stable | Streptomyces phaeochromogenes |
4.4.1.1 | 55 | - |
0.04 mM pyridoxal 5'-phosphate, 10 min, 15% loss of activity | Streptomyces phaeochromogenes |
4.4.1.1 | 60 | - |
0.04 mM pyridoxal 5'-phosphate, 10 min, 50% loss of activity | Streptomyces phaeochromogenes |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
4.4.1.1 | 9 | - |
- |
Streptomyces phaeochromogenes |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
4.4.1.1 | pyridoxal 5'-phosphate | contains 4 mol of pyridoxal 5'-phosphate per mol of enzyme | Streptomyces phaeochromogenes |