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Literature summary extracted from

  • Anderson, R.L.; Dahms, A.S.
    D-Fuconate dehydratase (1975), Methods Enzymol., 42C, 305-308.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
4.2.1.67 2-mercaptoethanol 2.8 mM, activation Pseudomonas sp.

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.2.1.67 CoCl2 13.3 mM, 18% residual activity Pseudomonas sp.
4.2.1.67 CuSO4 13.3 mM, 29% residual activity Pseudomonas sp.
4.2.1.67 EDTA
-
 Pseudomonas sp.
4.2.1.67 FeSO4 13.3 mM, 37% residual activity Pseudomonas sp.
4.2.1.67 NiCl2 13.3 mM, 8% residual activity Pseudomonas sp.
4.2.1.67 ZnCl2 13.3 mM, complete inactivation Pseudomonas sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.2.1.67 4
-
 D-fuconate
-
 Pseudomonas sp.
4.2.1.67 4.3
-
 L-arabonate
-
 Pseudomonas sp.

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.2.1.67 Mg2+ optimum: 20 mM, required Pseudomonas sp.

Organism

EC Number Organism UniProt Comment Textmining
4.2.1.67 Pseudomonas sp.
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.2.1.67
-
 Pseudomonas sp.

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.2.1.67 2.33
-
-
 Pseudomonas sp.

Storage Stability

EC Number Storage Stability Organism
4.2.1.67 0-4°C, 10% loss of activity after 1 week Pseudomonas sp.
4.2.1.67 frozen, 24 h, 50% loss of activity Pseudomonas sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.1.67 D-Fuconate
-
 Pseudomonas sp. 2-Dehydro-3-deoxy-D-fuconate + H2O
-
 ?
4.2.1.67 L-Arabonate
-
 Pseudomonas sp. 2-Keto-3-deoxy-L-arabonate + H2O
-
 ?
4.2.1.67 additional information not: L-fuconate, D-arabonate, D-galactonate, 6-iodo-6-deoxy-D-galactonate, D-ribonate, D-xylonate, D-lyxonate, D-mannonate, L-rhamnonate, D-gluconate, L-galactonate, L-gluconate, D-galactarate, D-glucarate, D-glucuronate, D-galacturonate, cellobionate Pseudomonas sp. ?
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.2.1.67 7.1 7.4
-
 Pseudomonas sp.