EC Number | Crystallization (Comment) | Organism |
---|---|---|
3.5.5.4 | crystallization in presence of (NH4)2SO4 | Pseudomonas sp. |
4.2.1.65 | - |
Pseudomonas sp. |
EC Number | General Stability | Organism |
---|---|---|
3.5.5.4 | 2-mercaptoethanol stabilizes | Pseudomonas sp. |
4.2.1.65 | very unstable, dithiothreitol, GSH and 2-mercaptoethanol protect | Pseudomonas sp. |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
3.5.5.4 | 1,10-phenanthroline | weak, 2-mercaptoethanol partially protects | Pseudomonas sp. | |
3.5.5.4 | 2,2'-dipyridyl | 2-mercaptoethanol partially protects | Pseudomonas sp. | |
3.5.5.4 | Ag2+ | 2-mercaptoethanol partially protects | Pseudomonas sp. | |
3.5.5.4 | Cd2+ | 2-mercaptoethanol partially protects | Pseudomonas sp. | |
3.5.5.4 | Co2+ | 2-mercaptoethanol partially protects | Pseudomonas sp. | |
3.5.5.4 | Cu2+ | 2-mercaptoethanol partially protects | Pseudomonas sp. | |
3.5.5.4 | Hg2+ | 2-mercaptoethanol partially protects | Pseudomonas sp. | |
3.5.5.4 | iodoacetamide | 2-mercaptoethanol partially protects | Pseudomonas sp. | |
3.5.5.4 | Mersalyl acid | strong | Pseudomonas sp. | |
3.5.5.4 | Mg2+ | weak | Pseudomonas sp. | |
3.5.5.4 | N-ethylmaleimide | 2-mercaptoethanol partially protects | Pseudomonas sp. | |
3.5.5.4 | NaF | 2-mercaptoethanol partially protects | Pseudomonas sp. | |
3.5.5.4 | NaNO3 | 2-mercaptoethanol partially protects | Pseudomonas sp. | |
3.5.5.4 | Ni2+ | weak | Pseudomonas sp. | |
3.5.5.4 | p-chloromercuribenzoate | strong | Pseudomonas sp. | |
3.5.5.4 | p-chloromercuriphenylsulfonic acid | strong | Pseudomonas sp. | |
4.2.1.65 | 2,2'-dipyridyl | poor | Pseudomonas sp. | |
4.2.1.65 | Ag+ | the inhibitory effect of metal ions is decreased in presence of 2-mercaptoethanol | Pseudomonas sp. | |
4.2.1.65 | Cd2+ | the inhibitory effect of metal ions is decreased in presence of 2-mercaptoethanol | Pseudomonas sp. | |
4.2.1.65 | Cu2+ | the inhibitory effect of metal ions is decreased in presence of 2-mercaptoethanol | Pseudomonas sp. | |
4.2.1.65 | HgCl2 | the inhibitory effect of metal ions is decreased in presence of 2-mercaptoethanol | Pseudomonas sp. | |
4.2.1.65 | iodoacetic acid | poor | Pseudomonas sp. | |
4.2.1.65 | Mersalyl acid | - |
Pseudomonas sp. | |
4.2.1.65 | N-ethylmaleimide | poor | Pseudomonas sp. | |
4.2.1.65 | NaF | poor | Pseudomonas sp. | |
4.2.1.65 | NaNO2 | poor | Pseudomonas sp. | |
4.2.1.65 | p-chloromercuribenzoate | - |
Pseudomonas sp. | |
4.2.1.65 | p-chloromercuriphenyl sulfonic acid | - |
Pseudomonas sp. |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.5.5.4 | 83 | - |
3-Cyano-L-alanine | pH 9.5, 37°C | Pseudomonas sp. | |
4.2.1.65 | 83 | - |
Beta-cyano-L-alanine | - |
Pseudomonas sp. |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
3.5.5.4 | 35000 | - |
30 * 35000, SDS-PAGE | Pseudomonas sp. |
3.5.5.4 | 1000000 | - |
ultracentrifugation | Pseudomonas sp. |
4.2.1.65 | 35000 | - |
x * 35000, SDS-PAGE | Pseudomonas sp. |
4.2.1.65 | 1000000 | - |
gel filtration | Pseudomonas sp. |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.5.5.4 | 3-cyano-L-alanine + H2O | Pseudomonas sp. | - |
L-aspartate + NH3 | L-asparagine is formed as an intermediate | ? | |
3.5.5.4 | 3-cyano-L-alanine + H2O | Pseudomonas sp. 13 | - |
L-aspartate + NH3 | L-asparagine is formed as an intermediate | ? | |
4.2.1.65 | beta-Cyano-L-alanine + H2O | Pseudomonas sp. | cyanide assimilation | ? | - |
? | |
4.2.1.65 | beta-Cyano-L-alanine + H2O | Pseudomonas sp. 13 | cyanide assimilation | ? | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.5.5.4 | Pseudomonas sp. | - |
- |
- |
3.5.5.4 | Pseudomonas sp. 13 | - |
- |
- |
4.2.1.65 | Pseudomonas sp. | - |
13 | - |
4.2.1.65 | Pseudomonas sp. 13 | - |
13 | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.5.5.4 | - |
Pseudomonas sp. |
4.2.1.65 | - |
Pseudomonas sp. |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
3.5.5.4 | 65 | - |
- |
Pseudomonas sp. |
4.2.1.65 | 54.7 | - |
- |
Pseudomonas sp. |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.5.5.4 | 3-cyano-L-alanine + H2O | - |
Pseudomonas sp. | L-aspartate + NH3 | L-asparagine is formed as an intermediate | ? | |
3.5.5.4 | 3-cyano-L-alanine + H2O | - |
Pseudomonas sp. 13 | L-aspartate + NH3 | L-asparagine is formed as an intermediate | ? | |
3.5.5.4 | additional information | no substrates are various nitrile or amide compounds, overview | Pseudomonas sp. | ? | - |
? | |
3.5.5.4 | additional information | no substrates are various nitrile or amide compounds, overview | Pseudomonas sp. 13 | ? | - |
? | |
4.2.1.65 | beta-Cyano-L-alanine + H2O | highly specific for beta-cyano-L-alanine | Pseudomonas sp. | L-Asparagine | - |
? | |
4.2.1.65 | beta-Cyano-L-alanine + H2O | highly specific for beta-cyano-L-alanine | Pseudomonas sp. 13 | L-Asparagine | - |
? | |
4.2.1.65 | beta-Cyano-L-alanine + H2O | cyanide assimilation | Pseudomonas sp. | ? | - |
? | |
4.2.1.65 | beta-Cyano-L-alanine + H2O | cyanide assimilation | Pseudomonas sp. 13 | ? | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
3.5.5.4 | polymer | 30 * 35000, SDS-PAGE | Pseudomonas sp. |
4.2.1.65 | ? | x * 35000, SDS-PAGE | Pseudomonas sp. |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.5.5.4 | 55 | - |
- |
Pseudomonas sp. |
4.2.1.65 | 55 | - |
- |
Pseudomonas sp. |
EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.5.5.4 | 30 | 62 | about half-maximal activity at 30°C and 62°C | Pseudomonas sp. |
4.2.1.65 | 20 | 65 | - |
Pseudomonas sp. |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.5.5.4 | 60 | - |
stable up to for 10 min in 50 mM phosphate buffer, pH 7.5 | Pseudomonas sp. |
4.2.1.65 | 60 | - |
up to, 10 min | Pseudomonas sp. |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.5.5.4 | 9.5 | - |
Tris-HCl buffer | Pseudomonas sp. |
4.2.1.65 | 9.5 | - |
- |
Pseudomonas sp. |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
3.5.5.4 | 8.2 | 10.8 | about half-maximal activity at pH 8.2 and pH 10.8 | Pseudomonas sp. |
4.2.1.65 | 7 | 11 | - |
Pseudomonas sp. |
EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|---|
3.5.5.4 | 7.5 | - |
3 h, stable at 37°C, only in the presence of 2-mercaptoethanol | Pseudomonas sp. |
4.2.1.65 | 7 | 11 | in presence of 2-mercaptoethanol | Pseudomonas sp. |