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Literature summary extracted from

  • Moskowitz, G.J.; Merrick, J.M.
    Metabolism of poly-beta-hydroxybutyrate. II. Enzymatic synthesis of D(-)-beta-hydroxybutyryl coenzyme A by an enoyl hydrase from Rhodospirillum rubrum (1969), Biochemistry, 8, 2748-2755.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.2.1.55 iodoacetamide 0.01 M, 30% inhibition Rhodospirillum rubrum
4.2.1.55 iodoacetic acid 0.01 M, 12% inhibition Rhodospirillum rubrum
4.2.1.55 N-ethylmaleimide 0.01 M, 23% inhibition Rhodospirillum rubrum
4.2.1.55 p-Substituted mercuribenzoate 3 mM, 65% inhibition Rhodospirillum rubrum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.2.1.55 0.00926
-
 crotonyl-CoA
-
 Rhodospirillum rubrum
4.2.1.55 0.0263
-
 trans-2-hexenoyl-CoA
-
 Rhodospirillum rubrum
4.2.1.55 0.08
-
 crotonyl-[acyl-carrier protein]
-
 Rhodospirillum rubrum
4.2.1.55 0.118
-
 Crotonylpantetheine
-
 Rhodospirillum rubrum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.2.1.17 crotonyl-CoA + H2O Rhodospirillum rubrum two enoyl coenzyme A hydrases ocur in Rhodospirillum rubrum extracts whose combined activity results in the racemization of (3S)-3-hydroxybutanoyl-CoA to (3R)-3-hydroxybutanoyl-CoA. Both hydrases catalyze the reversible hydration of crotonyl coenzyme A to 3-hydroxybutanoyl coenzyme A. One of the hydrases is specific for the synthesis of the (3S)-isomer (enoyl coenzyme A hydrase (D)) while the other catalyzes the synthesis of the (3R)-isomer (enoyl coenzyme A hydratase (L)) (3S)-3-hydroxybutanoyl-CoA
-
 r
4.2.1.55 crotonyl-CoA Rhodospirillum rubrum enzyme is involved in synthesis of poly-beta-hydroxybutyrate, lipid reserve of bacteria ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.2.1.17 Rhodospirillum rubrum
-
-
-
4.2.1.55 Rhodospirillum rubrum
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.2.1.55
-
 Rhodospirillum rubrum

Reaction

EC Number Reaction Comment Organism Reaction ID
4.2.1.55 (3R)-3-hydroxybutanoyl-CoA = crotonoyl-CoA + H2O stereochemistry Rhodospirillum rubrum
a

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.2.1.55 additional information
-
-
 Rhodospirillum rubrum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.1.17 crotonyl-CoA + H2O two enoyl coenzyme A hydrases ocur in Rhodospirillum rubrum extracts whose combined activity results in the racemization of (3S)-3-hydroxybutanoyl-CoA to (3R)-3-hydroxybutanoyl-CoA. Both hydrases catalyze the reversible hydration of crotonyl coenzyme A to 3-hydroxybutanoyl coenzyme A. One of the hydrases is specific for the synthesis of the (3S)-isomer (enoyl coenzyme A hydrase (D)) while the other catalyzes the synthesis of the (3R)-isomer (enoyl coenzyme A hydratase (L)) Rhodospirillum rubrum (3S)-3-hydroxybutanoyl-CoA
-
 r
4.2.1.17 crotonyl-CoA + H2O two enoyl coenzyme A hydrases occur in Rhodospirillum rubrum extracts whose combined activity results in the racemization of (3S)-3-hydroxybutanoyl-CoA to (3R)-3-hydroxybutanoyl-CoA. Both hydrases catalyze the reversible hydration of crotonyl-CoA to 3-hydroxybutanoyl-CoA. One of the hydrases is specific for the synthesis of the (3S)-isomer (enoyl coenzyme A hydrase (D)) while the other catalyzes the synthesis of the (3R)-isomer (enoyl coenzyme A hydratase (L)) Rhodospirillum rubrum (3S)-3-hydroxybutanoyl-CoA
-
 r
4.2.1.55 (3R)-3-hydroxybutanoyl-CoA
-
 Rhodospirillum rubrum crotonyl-CoA + H2O
-
 ?
4.2.1.55 crotonyl-CoA enzyme is involved in synthesis of poly-beta-hydroxybutyrate, lipid reserve of bacteria Rhodospirillum rubrum ?
-
 ?
4.2.1.55 Crotonyl-CoA + H2O
-
 Rhodospirillum rubrum (3R)-3-Hydroxybutanoyl-CoA
-
 ?
4.2.1.55 Crotonyl-[acyl-carrier protein] + H2O about 50% of the activity with crotonyl-CoA Rhodospirillum rubrum ?
-
 ?
4.2.1.55 Crotonylpantetheine about 55% of the activity with crotonyl-CoA Rhodospirillum rubrum ?
-
 ?
4.2.1.55 additional information no activity with crotonylglutathione, acrylyl-CoA Rhodospirillum rubrum ?
-
 ?
4.2.1.55 trans-2-Hexenoyl-CoA + H2O about 35% of the activity with crotonyl-CoA Rhodospirillum rubrum ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
4.2.1.17 enoyl coenzyme A hydratase (L)
-
 Rhodospirillum rubrum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.2.1.55 8.4 8.6
-
 Rhodospirillum rubrum