EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
6.2.1.1 | 2-mercaptoethanol | stimulates | Penicillium chrysogenum | |
6.2.1.1 | DTT | stimulates | Penicillium chrysogenum | |
6.2.1.1 | GSH | stimulates | Penicillium chrysogenum |
EC Number | General Stability | Organism |
---|---|---|
6.2.1.1 | particularly sensitive to repeated freezing | Penicillium chrysogenum |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
6.2.1.1 | 5,5'-dithiobis(2-nitrobenzoate) | - |
Penicillium chrysogenum | |
6.2.1.1 | Hg2+ | - |
Penicillium chrysogenum | |
6.2.1.1 | NEM | - |
Penicillium chrysogenum | |
6.2.1.1 | Nonidet P40 | weak | Penicillium chrysogenum | |
6.2.1.1 | p-chloromercuribenzoate | - |
Penicillium chrysogenum | |
6.2.1.1 | Tween 100 | - |
Penicillium chrysogenum | |
6.2.1.1 | Tween 20 | - |
Penicillium chrysogenum | |
6.2.1.1 | Tween 80 | - |
Penicillium chrysogenum | |
6.2.1.1 | Zn2+ | - |
Penicillium chrysogenum |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
6.2.1.1 | 0.18 | - |
CoA | - |
Penicillium chrysogenum | |
6.2.1.1 | 6.8 | - |
acetate | - |
Penicillium chrysogenum | |
6.2.1.1 | 17 | - |
ATP | - |
Penicillium chrysogenum |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
6.2.1.1 | Mg2+ | required | Penicillium chrysogenum | |
6.2.1.1 | Mn2+ | can replace Mg2+ in activation | Penicillium chrysogenum | |
6.2.1.1 | Mn2+ | 64% of the activation relative to Mg2+ | Penicillium chrysogenum |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
6.2.1.1 | 70000 | - |
2 * 70000, SDS-PAGE | Penicillium chrysogenum |
6.2.1.1 | 139000 | - |
gel filtration | Penicillium chrysogenum |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
6.2.1.1 | additional information | Aspergillus nidulans | the enzyme can catalyze the activation to their CoA thioesters of some of the side-chain precursors required in Penicillium chrysogenum and Aspergillus nidulans for the production of several penicillins | ? | - |
? | |
6.2.1.1 | additional information | Penicillium chrysogenum | the enzyme can catalyze the activation to their CoA thioesters of some of the side-chain precursors required in Penicillium chrysogenum and Aspergillus nidulans for the production of several penicillins | ? | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
6.2.1.1 | Aspergillus nidulans | - |
- |
- |
6.2.1.1 | Penicillium chrysogenum | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
6.2.1.1 | - |
Penicillium chrysogenum |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
6.2.1.1 | mycelium | - |
Penicillium chrysogenum | - |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
6.2.1.1 | 24 | - |
- |
Penicillium chrysogenum |
EC Number | Storage Stability | Organism |
---|---|---|
6.2.1.1 | -20°C, cannot be stored for more than 3 days without a considerable loss of activity | Penicillium chrysogenum |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
6.2.1.1 | ADP + acetate + CoA | 37% of the activity relative to ATP | Penicillium chrysogenum | AMP + diphosphate + acetyl-CoA | - |
? | |
6.2.1.1 | ATP + acetate + CoA | - |
Aspergillus nidulans | AMP + diphosphate + acetyl-CoA | - |
? | |
6.2.1.1 | ATP + acetate + CoA | - |
Penicillium chrysogenum | AMP + diphosphate + acetyl-CoA | - |
? | |
6.2.1.1 | ATP + butyrate + CoA | 20% of the activity relative to acetate | Penicillium chrysogenum | AMP + diphosphate + butyryl-CoA | - |
? | |
6.2.1.1 | ATP + pentanoate + CoA | 6.7% of the activity relative to acetate | Penicillium chrysogenum | AMP + diphosphate + pentanoyl-CoA | - |
? | |
6.2.1.1 | ATP + propanoate + CoA | 48% of the activity relative to acetate | Penicillium chrysogenum | AMP + diphosphate + propanoyl-CoA | - |
? | |
6.2.1.1 | CTP + acetate + CoA | - |
Penicillium chrysogenum | CMP + diphosphate + acetyl-CoA | - |
? | |
6.2.1.1 | GTP + acetate + CoA | - |
Penicillium chrysogenum | GMP + diphosphate + acetyl-CoA | - |
? | |
6.2.1.1 | additional information | the enzyme can activate many other molecules to acyl-CoA derivatives: hexanoate, 3-hexenoate, heptanoate, octanoate, 3-octenoate, phenylacetate, 2-thiophene acetate, 3-thiophene acetate | Penicillium chrysogenum | ? | - |
? | |
6.2.1.1 | additional information | the enzyme can catalyze the activation to their CoA thioesters of some of the side-chain precursors required in Penicillium chrysogenum and Aspergillus nidulans for the production of several penicillins | Aspergillus nidulans | ? | - |
? | |
6.2.1.1 | additional information | the enzyme can catalyze the activation to their CoA thioesters of some of the side-chain precursors required in Penicillium chrysogenum and Aspergillus nidulans for the production of several penicillins | Penicillium chrysogenum | ? | - |
? | |
6.2.1.1 | UTP + acetate + CoA | - |
Penicillium chrysogenum | UMP + diphosphate + acetyl-CoA | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
6.2.1.1 | dimer | 2 * 70000, SDS-PAGE | Penicillium chrysogenum |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
6.2.1.1 | 37 | - |
- |
Penicillium chrysogenum |
EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|---|
6.2.1.1 | 20 | 50 | 20°C: about 45% of maximal activity, 50°C: about 30% of maximal activity | Penicillium chrysogenum |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
6.2.1.1 | 8 | - |
- |
Penicillium chrysogenum |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
6.2.1.1 | 6 | 9.7 | 6.0: about 45% of maximal activity, 9.7: about 70% of maximal activity | Penicillium chrysogenum |