EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
1.1.1.18 | 2-keto-myo-inositol | - |
Klebsiella aerogenes | |
4.2.1.44 | EDTA | inhibition can be alleviated by either Mn2+ or Co2+ | Klebsiella aerogenes | |
4.2.1.44 | p-hydroxymercuribenzoate | on addition of glutathione or mercaptoethanol the activity is regained | Klebsiella aerogenes |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
4.2.1.44 | 0.15 | - |
2-keto-myo-inositol | - |
Klebsiella aerogenes |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
4.2.1.44 | Co2+ | increases activity | Klebsiella aerogenes | |
4.2.1.44 | Mn2+ | increases activity | Klebsiella aerogenes |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.1.18 | D-2,3-diketo-4-deoxy-epi-inositol + NADH | Klebsiella aerogenes | - |
ketodeoxyinositol + NAD+ | - |
? | |
1.1.1.18 | myo-inositol + NAD+ | Klebsiella aerogenes | - |
2,4,6/3,5-pentahydroxycyclohexanone + NADH | - |
? | |
4.2.1.44 | (2R,3S,4s,5R,6S)-2,3,4,5,6-pentahydroxycyclohexanone | Klebsiella aerogenes | pathway of myo-inositol degradation | (3R,4S,5R)-3,4,5-trihydroxycyclohexane-1,2-dione + H2O | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.1.1.18 | Klebsiella aerogenes | - |
- |
- |
4.2.1.44 | Klebsiella aerogenes | - |
- |
- |
EC Number | Oxidation Stability | Organism |
---|---|---|
4.2.1.44 | unstable when exposed to oxygen | Klebsiella aerogenes |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.1.1.18 | partial | Klebsiella aerogenes |
4.2.1.44 | partial | Klebsiella aerogenes |
EC Number | Reaction | Comment | Organism | Reaction ID |
---|---|---|---|---|
4.2.1.44 | 2,4,6/3,5-Pentahydroxycyclohexanone = 3,5/4-trihydroxycyclohexa-1,2-dione + H2O | mechanism | Klebsiella aerogenes |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
1.1.1.18 | cell culture | - |
Klebsiella aerogenes | - |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
1.1.1.18 | 280 | - |
- |
Klebsiella aerogenes |
4.2.1.44 | 270 | - |
- |
Klebsiella aerogenes |
EC Number | Storage Stability | Organism |
---|---|---|
1.1.1.18 | -10°C, several months | Klebsiella aerogenes |
4.2.1.44 | -10°C, nitrogen atmosphere, for 1 month, about 50% loss of activity | Klebsiella aerogenes |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.1.18 | D-2,3-diketo-4-deoxy-epi-inositol + NADH | - |
Klebsiella aerogenes | ketodeoxyinositol + NAD+ | - |
? | |
1.1.1.18 | myo-inositol + NAD+ | - |
Klebsiella aerogenes | 2,4,6/3,5-pentahydroxycyclohexanone + NADH | - |
? | |
4.2.1.44 | (2R,3S,4s,5R,6S)-2,3,4,5,6-pentahydroxycyclohexanone | - |
Klebsiella aerogenes | (3R,4S,5R)-3,4,5-trihydroxycyclohexane-1,2-dione + H2O | - |
? | |
4.2.1.44 | (2R,3S,4s,5R,6S)-2,3,4,5,6-pentahydroxycyclohexanone | pathway of myo-inositol degradation | Klebsiella aerogenes | (3R,4S,5R)-3,4,5-trihydroxycyclohexane-1,2-dione + H2O | - |
? | |
4.2.1.44 | L-2-Keto-epi-inositol | - |
Klebsiella aerogenes | ? | - |
? |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
4.2.1.44 | 6 | 6.2 | 2-keto-myo-inositol | Klebsiella aerogenes |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.1.1.18 | NAD+ | preferred hydrogen acceptor | Klebsiella aerogenes | |
1.1.1.18 | NADH | - |
Klebsiella aerogenes |