Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Wang, C.C.; Barker, H.A.
    L-Citramalate hydrolyase (1964), Methods Enzymol., 13, 331-344.
No PubMed abstract available

Activating Compound

EC Number Activating Compound Comment Organism Structure
4.2.1.34 2,3-dimercapto-1-propanol about half as effective as L-Cys Clostridium tetanomorphum
4.2.1.34 2-mercaptoethylamine at pH 8.2-8.4 as effective as L-Cys Clostridium tetanomorphum
4.2.1.34 D-Cys at pH 8.2-8.4 as effective as L-Cys Clostridium tetanomorphum
4.2.1.34 DL-homocysteine at pH 8.2-8.4 as effective as L-Cys Clostridium tetanomorphum
4.2.1.34 L-Cys required. The optimal concentration of L-Cys is 0.5-2.0 mM Clostridium tetanomorphum
4.2.1.34 sulfhydryl compounds required Clostridium tetanomorphum

General Stability

EC Number General Stability Organism
4.2.1.34 freezing and thawing does not significantly decrease the activity of component I, 10-15% loss of activity of component II Clostridium tetanomorphum

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.2.1.34 D-malate
-
 Clostridium tetanomorphum
4.2.1.34 L-Tartrate
-
 Clostridium tetanomorphum
4.2.1.34 Mn2+
-
 Clostridium tetanomorphum
4.2.1.34 succinate
-
 Clostridium tetanomorphum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.2.1.34 0.59
-
 mesaconate
-
 Clostridium tetanomorphum
4.2.1.34 0.95
-
 fumarate
-
 Clostridium tetanomorphum
4.2.1.34 2.4
-
 L-citramalate
-
 Clostridium tetanomorphum
4.2.1.34 6.7
-
 L-malate
-
 Clostridium tetanomorphum

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.2.1.34 Fe2+ maximal activity at 0.2-0.9 mM Clostridium tetanomorphum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.2.1.34 37000
-
 enzyme consists of 2 components, neither component alone possesses hydrolase activity, MW of component I: 46000 Da, gel filtration, MW of component I: 310000 Da, gel filtration, 8 * 37000, SDS-PAGE Clostridium tetanomorphum

Organism

EC Number Organism UniProt Comment Textmining
4.2.1.34 Clostridium tetanomorphum
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.2.1.34 component I and II Clostridium tetanomorphum

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.2.1.34 additional information
-
 of component I and II Clostridium tetanomorphum

Storage Stability

EC Number Storage Stability Organism
4.2.1.34 -10°C, pH 8.4, protein concentration above 1 mg/ml in 0.1 M Tris-HCl buffer, component I is stable for months Clostridium tetanomorphum
4.2.1.34 -196°C, activity of component II remains constant for at least 8 months Clostridium tetanomorphum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.1.34 (L)-citramalate
-
 Clostridium tetanomorphum mesaconate + H2O
-
 ?
4.2.1.34 (S)-malate
-
 Clostridium tetanomorphum fumarate + H2O
-
 ?
4.2.1.34 Citramalate + H2O
-
 Clostridium tetanomorphum Mesaconate
-
 ?
4.2.1.34 fumarate + H2O
-
 Clostridium tetanomorphum L-malate
-
 ?
4.2.1.34 mesaconate
-
 Clostridium tetanomorphum citramalate + H2O
-
 ?

Subunits

EC Number Subunits Comment Organism
4.2.1.34 ? enzyme consists of 2 components, neither component alone possesses hydrolase activity, MW of component I: 46000 Da, gel filtration, MW of component I: 310000 Da, gel filtration, 8 * 37000, SDS-PAGE Clostridium tetanomorphum

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
4.2.1.34 37
-
 2 d, component II is stable in presence of mercaptoethanol, Fe2+, and component I Clostridium tetanomorphum
4.2.1.34 55
-
 5 min, component II is stable in presence of mercaptoethanol and Fe2+ Clostridium tetanomorphum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.2.1.34 6.5 7 in presence of Cys Clostridium tetanomorphum
4.2.1.34 8 8.5 in presence of 2-mercaptoethanol Clostridium tetanomorphum

pH Range

EC Number pH Minimum pH Maximum Comment Organism
4.2.1.34 5.7 7.7 about 50% of maximal activity at pH 7.1 and at pH 9.3, in presence of Cys Clostridium tetanomorphum
4.2.1.34 7.1 9.3 about 50% of maximal activity at pH 7.1 and at pH 9.3, in presence of 2-mercaptoethanol Clostridium tetanomorphum

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
4.2.1.34 7 11 component I is stable Clostridium tetanomorphum
4.2.1.34 7 8 4°C, 48 h, about 20% loss of activity of component II Clostridium tetanomorphum