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Literature summary extracted from

  • Wang, C.C.; Barker, H.A.
    Purification and properties of L-citramalate hydrolyase (1969), J. Biol. Chem., 244, 2516-2526.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
4.2.1.34 sulfhydryl compounds required Clostridium tetanomorphum

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.2.1.34 D-malate competitive with L-malate Clostridium tetanomorphum
4.2.1.34 L-Tartrate
-
 Clostridium tetanomorphum
4.2.1.34 MnCl2
-
 Clostridium tetanomorphum
4.2.1.34 succinate
-
 Clostridium tetanomorphum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.2.1.34 0.59
-
 mesaconate
-
 Clostridium tetanomorphum
4.2.1.34 0.95
-
 fumarate
-
 Clostridium tetanomorphum
4.2.1.34 2.4
-
 L-citramalate
-
 Clostridium tetanomorphum
4.2.1.34 6.7
-
 L-malate
-
 Clostridium tetanomorphum

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.2.1.34 Fe2+ activates Clostridium tetanomorphum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.2.1.34 additional information
-
 the enzyme consists of two components: component I has MW 45000 and component II has MW 310000, determined by gel filtration. A combination of both components gives the active enzyme. At pH 7.5 or below component II partially dissociates into species with molecular weights of about 165000, 82000, and 37000. At pH 9.0 these species reassociate to form active component II of about the original MW. The observed species may represent a monomer, dimer, tetramer and octamer of a single subunit Clostridium tetanomorphum

Organism

EC Number Organism UniProt Comment Textmining
4.2.1.34 Clostridium tetanomorphum
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.2.1.34 component I and II Clostridium tetanomorphum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.1.34 (L)-citramalate
-
 Clostridium tetanomorphum mesaconate + H2O
-
 ?
4.2.1.34 (S)-malate
-
 Clostridium tetanomorphum fumarate + H2O
-
 ?
4.2.1.34 Citramalate + H2O L-citramalate Clostridium tetanomorphum Mesaconate
-
 ?
4.2.1.34 fumarate + H2O
-
 Clostridium tetanomorphum L-malate
-
 ?
4.2.1.34 mesaconate
-
 Clostridium tetanomorphum citramalate + H2O
-
 ?

Subunits

EC Number Subunits Comment Organism
4.2.1.34 ? the enzyme consists of two components: component I has MW 45000 and component II has MW 310000, determined by gel filtration. A combination of both components gives the active enzyme. At pH 7.5 or below component II partially dissociates into species with molecular weights of about 165000, 82000, and 37000. At pH 9.0 these species reassociate to form active component II of about the original MW. The observed species may represent a monomer, dimer, tetramer and octamer of a single subunit Clostridium tetanomorphum

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
4.2.1.34 7.5
-
 at pH 7.5 or below component II partially dissociates into species with molecular weights of about 165000 Da, 82000 Da, and 37000 Da. At pH 9.0 these species reassociate to form active component II of about the original MW. The observed species may represent a monomer, dimer, tetramer and octamer of a single subunit Clostridium tetanomorphum