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Literature summary extracted from

  • Van der Werf, M.J.; Van der Tweel, W.J.J.; Hartmans, S.
    Purification and characterization of maleate hydratase from Pseudomonas pseudoalcaligenes (1993), Appl. Environ. Microbiol., 59, 2823-2829.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
4.2.1.31 No requirement
-
 Pseudomonas oleovorans

General Stability

EC Number General Stability Organism
4.2.1.31 DL-malate stabilizes Pseudomonas oleovorans

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.2.1.31 2,2-dimethylsuccinate Ki: 0.025 mM Pseudomonas oleovorans
4.2.1.31 CuSO4 60% inhibition at 1 mM Pseudomonas oleovorans
4.2.1.31 D-citramalate Ki: 0.083 mM Pseudomonas oleovorans
4.2.1.31 D-malate Ki: 0.63 mM Pseudomonas oleovorans
4.2.1.31 Fe2(SO4)3 67% inhibition at 1 mM Pseudomonas oleovorans
4.2.1.31 HgCl2 complete inhibition at 1 mM Pseudomonas oleovorans
4.2.1.31 iodoacetamide complete inhibition at 1 mM Pseudomonas oleovorans
4.2.1.31 additional information
-
 Pseudomonas oleovorans
4.2.1.31 p-chloromercuribenzoate complete inhibition at 0.1 mM Pseudomonas oleovorans
4.2.1.31 ZnSO4 complete inhibition at 1 mM Pseudomonas oleovorans

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.2.1.31 0.2
-
 citraconate
-
 Pseudomonas oleovorans
4.2.1.31 0.35
-
 Maleate
-
 Pseudomonas oleovorans

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.2.1.31 24000
-
 alpha1 beta1, 1 * 57000 + 1 * 24000, SDS-PAGE Pseudomonas oleovorans
4.2.1.31 57000
-
 alpha1 beta1, 1 * 57000 + 1 * 24000, SDS-PAGE Pseudomonas oleovorans
4.2.1.31 89000
-
 gel filtration Pseudomonas oleovorans

Organism

EC Number Organism UniProt Comment Textmining
4.2.1.31 Pseudomonas oleovorans
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.2.1.31
-
 Pseudomonas oleovorans

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.2.1.31 108
-
-
 Pseudomonas oleovorans

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.1.31 2,3-dimethylmaleate 0.8% of turnover of maleate, equlibrium constant: 11.2 Pseudomonas oleovorans ?
-
 ?
4.2.1.31 citraconate 54% of turnover of maleate, equlibrium constant: 104 Pseudomonas oleovorans D-citramalate
-
 ?
4.2.1.31 maleate equilibrium constant: 2050 Pseudomonas oleovorans (R)-malate
-
 ?

Subunits

EC Number Subunits Comment Organism
4.2.1.31 dimer alpha1 beta1, 1 * 57000 + 1 * 24000, SDS-PAGE Pseudomonas oleovorans

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.2.1.31 30
-
 enzyme assay at Pseudomonas oleovorans
4.2.1.31 45
-
-
 Pseudomonas oleovorans

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.2.1.31 7.5
-
 enzyme assay at Pseudomonas oleovorans
4.2.1.31 8
-
-
 Pseudomonas oleovorans

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
4.2.1.31 0.025
-
 2,2-dimethylsuccinate
-
 Pseudomonas oleovorans
4.2.1.31 0.083
-
 D-citramalate
-
 Pseudomonas oleovorans