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Literature summary extracted from

  • Van der Werf, M.J.; Hartmans, S.; Van der Tweel, W.J.J.
    Effect of maleate counterion on malease activity: production of D-malate in a crystal-liquid two-phase system (1995), Enzyme Microb. Technol., 17, 430-436.
No PubMed abstract available

Application

EC Number Application Comment Organism
4.2.1.31 synthesis biosynthesis of synthons for fine chemicals industry, description of a crystal-liquid two-phase system and soluble metal-organic acid complex system for fermentation which minimizes substrate inhibition and thereby increases efficiency Pseudomonas oleovorans

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.2.1.31 Ba2+ 36% inhibition at 100 mM, formation of substrate/product precipitate Pseudomonas oleovorans
4.2.1.31 Ca2+ 20% inhibition at 250 mM Pseudomonas oleovorans
4.2.1.31 D-malate important for commercial use, liquid/crystal two phase systems developed to minimize effect Pseudomonas oleovorans
4.2.1.31 iodoacetamide
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Pseudomonas oleovorans
4.2.1.31 Maleate important for commercial use, liquid/crystal two phase systems developed to minimize effect Pseudomonas oleovorans
4.2.1.31 Mg2+ 16% inhibition at 250 mM Pseudomonas oleovorans
4.2.1.31 additional information
-
Pseudomonas oleovorans

Organism

EC Number Organism UniProt Comment Textmining
4.2.1.31 Pseudomonas oleovorans
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.1.31 citraconate
-
Pseudomonas oleovorans D-citramalate
-
?
4.2.1.31 additional information maleate counterion greatly affects enzyme activity and solute substrate concentration, Ca2+ at 750 mM optimal, followed by Mg2+ Pseudomonas oleovorans ?
-
?