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Literature summary extracted from

  • McGee, D.E.; Carroll, S.S.; Bond, M.W.
    Diol dehydratase: N-terminal amino acid sequences and subunit stoichiometry (1982), Biochem. Biophys. Res. Commun., 108, 547-551.
    View publication on PubMed

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
4.2.1.28 cell membrane peripheral membrane protein Klebsiella pneumoniae
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.2.1.28 K+
-
 Klebsiella pneumoniae

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.2.1.28 15000
-
 2 * 60000, 1 * 51000, 2 * 29000, 2 * 15000, SDS-PAGE, subunit stoichiometry confirmed by N-terminal amino acid analysis Klebsiella pneumoniae
4.2.1.28 29000
-
 2 * 60000, 1 * 51000, 2 * 29000, 2 * 15000, SDS-PAGE, subunit stoichiometry confirmed by N-terminal amino acid analysis Klebsiella pneumoniae
4.2.1.28 51000
-
 2 * 60000, 1 * 51000, 2 * 29000, 2 * 15000, SDS-PAGE, subunit stoichiometry confirmed by N-terminal amino acid analysis Klebsiella pneumoniae
4.2.1.28 60000
-
 2 * 60000, 1 * 51000, 2 * 29000, 2 * 15000, SDS-PAGE, subunit stoichiometry confirmed by N-terminal amino acid analysis Klebsiella pneumoniae

Organism

EC Number Organism UniProt Comment Textmining
4.2.1.28 Klebsiella pneumoniae
-
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.2.1.28 82.2
-
-
 Klebsiella pneumoniae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.1.28 1,2-propanediol
-
 Klebsiella pneumoniae propanal
-
 ?

Subunits

EC Number Subunits Comment Organism
4.2.1.28 heptamer 2 * 60000, 1 * 51000, 2 * 29000, 2 * 15000, SDS-PAGE, subunit stoichiometry confirmed by N-terminal amino acid analysis Klebsiella pneumoniae
4.2.1.28 More role of 60000 and 29000 subunits as membrane anchors, role of 51000 subunit for coenzyme binding Klebsiella pneumoniae

Cofactor

EC Number Cofactor Comment Organism Structure
4.2.1.28 cobamide
-
 Klebsiella pneumoniae