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Literature summary extracted from

  • Miles, E.W.; Bauerle, R.; Ahmed S.A.
    Tryptophan synthase from Escherichia coli and Salmonella thyphimurium (1987), Methods Enzymol., 142, 398-414.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.2.1.20 6-azido-L-tryptophan
-
 Escherichia coli
4.2.1.20 6-azido-L-tryptophan
-
 Salmonella enterica subsp. enterica serovar Typhimurium
4.2.1.20 trans-L-2-amino-4-methoxy-3-butenoic acid
-
 Escherichia coli
4.2.1.20 trans-L-2-amino-4-methoxy-3-butenoic acid
-
 Salmonella enterica subsp. enterica serovar Typhimurium

Organism

EC Number Organism UniProt Comment Textmining
4.2.1.20 Escherichia coli
-
-
-
4.2.1.20 Salmonella enterica subsp. enterica serovar Typhimurium
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.2.1.20 alpha2beta2 holoenzyme, beta2 holoenzyme, beta2 apoenzyme, alpha subunit Salmonella enterica subsp. enterica serovar Typhimurium
4.2.1.20 alpha2beta2 holoenzyme, beta2 holoenzyme, beta2 apoenzyme, alpha subunit Escherichia coli

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.2.1.20 125
-
 indole-3-glycerol phosphate + L-serine Salmonella enterica subsp. enterica serovar Typhimurium
4.2.1.20 125
-
 indole-3-glycerol phosphate + L-serine Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.1.20 1-(indol-3-yl)glycerol 3-phosphate + L-serine catalyzed by alpha2beta2 holoenzyme Salmonella enterica subsp. enterica serovar Typhimurium L-tryptophan + D-glyceraldehyde 3-phosphate + H2O
-
 ?
4.2.1.20 1-(indol-3-yl)glycerol 3-phosphate + L-serine catalyzed by alpha2beta2 holoenzyme Escherichia coli L-tryptophan + D-glyceraldehyde 3-phosphate + H2O
-
 ?
4.2.1.20 2-amino-3-butenoic acid vinylglycine Salmonella enterica subsp. enterica serovar Typhimurium 2-oxobutyric acid + NH3
-
 ?
4.2.1.20 2-amino-3-butenoic acid vinylglycine Escherichia coli 2-oxobutyric acid + NH3
-
 ?
4.2.1.20 2-mercaptoethanol + L-serine + pyridoxal phosphate
-
 Salmonella enterica subsp. enterica serovar Typhimurium S-pyruvylmercaptoethanol + pyridoxamine phosphate + H2O
-
 ?
4.2.1.20 2-mercaptoethanol + L-serine + pyridoxal phosphate
-
 Escherichia coli S-pyruvylmercaptoethanol + pyridoxamine phosphate + H2O
-
 ?
4.2.1.20 indole + L-serine catalyzed by beta2 subunit Salmonella enterica subsp. enterica serovar Typhimurium L-tryptophan + H2O
-
 ?
4.2.1.20 indole + L-serine catalyzed by beta2 subunit Escherichia coli L-tryptophan + H2O
-
 ?
4.2.1.20 indole + L-serine OH of Ser can be replaced by SCH3, OCH3 and Cl, but not by indole, indole can be replaced by CH3SH, CH2OHCH2SH, thiobenzyl alcohol, 1-propanethiol, 1-butanethiol, selenols, 6-azidoindole Salmonella enterica subsp. enterica serovar Typhimurium L-tryptophan + H2O
-
 ?
4.2.1.20 indole + L-serine OH of Ser can be replaced by SCH3, OCH3 and Cl, but not by indole, indole can be replaced by CH3SH, CH2OHCH2SH, thiobenzyl alcohol, 1-propanethiol, 1-butanethiol, selenols, 6-azidoindole Escherichia coli L-tryptophan + H2O
-
 ?
4.2.1.20 indole-3-glycerol phosphate catalyzed by alpha-subunit Salmonella enterica subsp. enterica serovar Typhimurium indole + D-glyceraldehyde 3-phosphate
-
 ?
4.2.1.20 indole-3-glycerol phosphate catalyzed by alpha-subunit Escherichia coli indole + D-glyceraldehyde 3-phosphate
-
 ?
4.2.1.20 L-serine catalyzed by beta2-subunit Salmonella enterica subsp. enterica serovar Typhimurium pyruvate + NH3
-
 ?
4.2.1.20 L-serine catalyzed by beta2-subunit Escherichia coli pyruvate + NH3
-
 ?
4.2.1.20 L-serine OH of Ser can be replaced by SCH3, OCH3 and Cl, but not by indole Salmonella enterica subsp. enterica serovar Typhimurium pyruvate + NH3
-
 ?
4.2.1.20 L-serine OH of Ser can be replaced by SCH3, OCH3 and Cl, but not by indole Escherichia coli pyruvate + NH3
-
 ?

Cofactor

EC Number Cofactor Comment Organism Structure
4.2.1.20 pyridoxal 5'-phosphate 2 mol bound per mol of beta2 subunit Salmonella enterica subsp. enterica serovar Typhimurium
4.2.1.20 pyridoxal 5'-phosphate 2 mol bound per mol of beta2 subunit Escherichia coli