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Literature summary extracted from

  • Roberts, J.R.; Narasimhan, C.; Hruz, P.W.; Mitchell, G.A.; Miziorko, H.M.
    3-Hydroxy-3-methylglutaryl-CoA lyase: expression and isolation of the recombinant human enzyme and investigation of a mechanism for regulation of enzyme activity (1994), J. Biol. Chem., 269, 17841-17846.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.1.3.4 expressed in Escherichia coli Homo sapiens
4.1.3.4 overexpression of wild-type enzyme and synthetic C323 mutant Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.1.3.4 additional information C323 involved in intersubunit cross-linking upon oxidative inactivation, thiol-disulfide exchange mechanism Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.1.3.4 0.024
-
 hydroxymethylglutaryl-CoA wild-type Homo sapiens
4.1.3.4 0.045
-
 hydroxymethylglutaryl-CoA C323S mutant Homo sapiens

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.1.3.4 Mg2+
-
 Homo sapiens
4.1.3.4 Mn2+
-
 Homo sapiens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.1.3.4 31600
-
 x * 31600, estimated from nucleotide sequence Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
4.1.3.4 Homo sapiens
-
 recombinant enzyme
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.1.3.4 recombinant wild-type enzyme and C323S mutant Homo sapiens

Reaction

EC Number Reaction Comment Organism Reaction ID
4.1.3.4 (S)-3-Hydroxy-3-methylglutaryl-CoA = acetyl-CoA + acetoacetate identification of reactive cysteines, possibly regulatory mechanism via thiol/disulfide exchange Homo sapiens
a

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.1.3.4 159
-
 wild-type Homo sapiens
4.1.3.4 348
-
 C323S mutant Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.3.4 3-hydroxy-3-methylglutaryl-CoA
-
 Homo sapiens acetoacetate + acetyl-CoA
-
 ?

Subunits

EC Number Subunits Comment Organism
4.1.3.4 ? x * 31600, estimated from nucleotide sequence Homo sapiens
4.1.3.4 More C323 involved in intersubunit cross-linking upon oxidative inactivation Homo sapiens