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Literature summary extracted from

  • Sarantoglou, V.; Imbault, P.; Weil, J.H.
    The use of affinity elution from blue dextran Sepharose by yeast tRNA2Val in the complete purification of the cytoplasmic valyl-tRNA synthetase from Euglena gracilis (1980), Biochem. Biophys. Res. Commun., 93, 134-140.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.1.1.9 0.00005
-
 tRNAVal of Euglena gracilis Euglena gracilis
6.1.1.9 0.00008
-
 tRNAVal of Euglena, , cytoplasmic enzyme Euglena gracilis
6.1.1.9 0.00018
-
 tRNAVal substrate from cytoplasm, , cytoplasmic enzyme Euglena gracilis
6.1.1.9 0.05
-
 L-valine
-
 Euglena gracilis
6.1.1.9 0.07
-
 ATP
-
 Euglena gracilis

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
6.1.1.9 cytoplasm
-
 Euglena gracilis 5737
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
6.1.1.9 126000
-
 gel filtration Euglena gracilis

Organism

EC Number Organism UniProt Comment Textmining
6.1.1.9 Euglena gracilis
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.1.1.9
-
 Euglena gracilis

Source Tissue

EC Number Source Tissue Comment Organism Textmining

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
6.1.1.9 0.38
-
-
 Euglena gracilis

Storage Stability

EC Number Storage Stability Organism
6.1.1.9 4°C, 1 month, less than 20% loss of activity Euglena gracilis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.1.1.9 ATP + L-valine + tRNAVal
-
 Euglena gracilis AMP + diphosphate + L-valyl-tRNAVal
-
 ?

Subunits

EC Number Subunits Comment Organism
6.1.1.9 monomer 1 * 126000, urea-SDS-PAGE Euglena gracilis