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Literature summary extracted from

  • Ashmarina, L.I.; Robert, M.F.; Elsliger, M.A.; Mitchell, G.A.
    Characterization of the hydroxymethylglutaryl-CoA lyase precursor, a protein targeted to peroxisomes and mitochondria (1996), Biochem. J., 315, 71-75.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.1.3.4 expressed in Escherichia coli Homo sapiens
4.1.3.4 mitochondrial and peroxisomal enzyme Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.1.3.4 0.0448
-
 hydroxymethylglutaryl-CoA both isoenzymes Homo sapiens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
4.1.3.4 peroxisome enzyme differs from mitochondrial enzyme according to immunoreactivity and other properties, mitochondrial leader sequence influences quaternary structure Homo sapiens 5777
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.1.3.4 32000
-
 2 * 32000, mitochondrial enzyme, SDS-PAGE Homo sapiens
4.1.3.4 34100
-
 peroxisomal enzyme, gel filtration Homo sapiens
4.1.3.4 34500
-
 1 * 34500, peroxisomal enzyme, SDS-PAGE Homo sapiens
4.1.3.4 65000
-
 mitochondrial enzyme, gel filtration Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
4.1.3.4 Homo sapiens
-
 human, mitochondrial and peroxisomal enzyme
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.1.3.4 mitochondrial and peroxisomal enzyme Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.1.3.4 220
-
 peroxisomal enzyme Homo sapiens
4.1.3.4 249
-
 mitochondrial enzyme Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.3.4 3-hydroxy-3-methylglutaryl-CoA
-
 Homo sapiens acetoacetate + acetyl-CoA
-
 ?

Subunits

EC Number Subunits Comment Organism
4.1.3.4 dimer 2 * 32000, mitochondrial enzyme, SDS-PAGE Homo sapiens
4.1.3.4 monomer 1 * 34500, peroxisomal enzyme, SDS-PAGE Homo sapiens

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.1.3.4 0.105
-
 (S)-3-hydroxy-3-methylglutaryl-CoA peroxisomal enzyme Homo sapiens
4.1.3.4 0.127
-
 (S)-3-hydroxy-3-methylglutaryl-CoA mitochondrial enzyme Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.1.3.4 9 9.5 both isoenzymes Homo sapiens