Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Natarajan, V.; Gopinathan, K.P.
    Purification and properties of valyl-tRNA synthetase from Mycobacterium smegmatis (1979), Biochim. Biophys. Acta, 568, 253-263.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
6.1.1.9 L-Penicillamine
-
 Mycolicibacterium smegmatis
6.1.1.9 p-hydroxymercuribenzoate
-
 Mycolicibacterium smegmatis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.1.1.9 0.001
-
 valine tRNA Mycolicibacterium smegmatis
6.1.1.9 0.00125
-
 valine
-
 Mycolicibacterium smegmatis
6.1.1.9 0.1
-
 ATP
-
 Mycolicibacterium smegmatis

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
6.1.1.9 Ca2+ at 5 mM, 16% of the activation with Mg2+ Mycolicibacterium smegmatis
6.1.1.9 Mg2+ optimal concentration: 5 mM Mycolicibacterium smegmatis
6.1.1.9 Mn2+ 60% of the activation with Mg2+ Mycolicibacterium smegmatis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
6.1.1.9 30000
-
 4 * 30000, SDS-PAGE Mycolicibacterium smegmatis
6.1.1.9 126000
-
 gel filtration Mycolicibacterium smegmatis

Organism

EC Number Organism UniProt Comment Textmining
6.1.1.9 Mycolicibacterium smegmatis
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.1.1.9
-
 Mycolicibacterium smegmatis

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
6.1.1.9 0.0042
-
-
 Mycolicibacterium smegmatis

Storage Stability

EC Number Storage Stability Organism
6.1.1.9 0°C, stable for several months Mycolicibacterium smegmatis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.1.1.9 ATP + L-valine + tRNAVal tRNA from E. coli or Mycobacterium smegmatis Mycolicibacterium smegmatis AMP + diphosphate + L-valyl-tRNAVal
-
 ?
6.1.1.9 additional information no activity with dATP Mycolicibacterium smegmatis ?
-
 ?

Subunits

EC Number Subunits Comment Organism
6.1.1.9 tetramer 4 * 30000, SDS-PAGE Mycolicibacterium smegmatis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
6.1.1.9 35 40
-
 Mycolicibacterium smegmatis

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
6.1.1.9 60
-
 1 min, stable Mycolicibacterium smegmatis
6.1.1.9 70
-
 1 min, complete inactivation Mycolicibacterium smegmatis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
6.1.1.9 6.8 7
-
 Mycolicibacterium smegmatis