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Literature summary extracted from

  • Bergmann, F.H.; Berg, P.; Dieckmann, M.
    The Enzymic synthesis of amino acyl derivatives of ribonucleic acid. II. The preparation of leucyl-, valyl-, isoleucyl-, and methionyl ribonucleic acid synthetases from Escherichia coli (1961), J. Biol. Chem., 236, 1735-1740.
No PubMed abstract available

Activating Compound

EC Number Activating Compound Comment Organism Structure
6.1.1.9 2-mercaptoethanol stimulates Escherichia coli

General Stability

EC Number General Stability Organism
6.1.1.4 unstable in the absence of sulfhydryl containing compound Escherichia coli
6.1.1.9 relatively unstable in the absence of a sulfhydryl-containing compound Escherichia coli

Inhibitors

EC Number Inhibitors Comment Organism Structure
6.1.1.9 D-allo-2-Amino-3-chlorobutyrate
-
 Escherichia coli
6.1.1.9 DL-2-amino-3-chlorobutyrate
-
 Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.1.1.4 0.05
-
 L-Leu
-
 Escherichia coli
6.1.1.9 0.1
-
 valine
-
 Escherichia coli
6.1.1.9 0.33
-
 DL-2-amino-3-chlorobutyrate
-
 Escherichia coli
6.1.1.9 1
-
 DL-allo-2-amino-3-chlorobutyrate
-
 Escherichia coli
6.1.1.9 3.7
-
 DL-2-aminobutyrate
-
 Escherichia coli
6.1.1.9 12
-
 DL-threonine
-
 Escherichia coli

Organism

EC Number Organism UniProt Comment Textmining
6.1.1.4 Escherichia coli
-
 B or ML30
-
6.1.1.9 Escherichia coli
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.1.1.9
-
 Escherichia coli

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
6.1.1.9 0.01
-
-
 Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.1.1.4 Adenylyl beta,gamma-imido diphosphonate + L-leucine + tRNALeu
-
 Escherichia coli Adenylic acid + imido-diphosphate + L-leucyl-tRNALeu
-
 ?
6.1.1.4 ATP + L-leucine + tRNALeu
-
 Escherichia coli AMP + diphosphate + L-leucyl-tRNALeu
-
 ?
6.1.1.4 additional information leucine-dependent ATP-diphosphate exchange, leucine + ATP + enzyme/Ile-AMP-enzyme + diphosphate Escherichia coli ?
-
 ?
6.1.1.9 ATP + DL-2-amino-3-chlorobutyrate + tRNAVal as effective as valine Escherichia coli AMP + diphosphate + DL-2-amino-3-chlorobutyryl-tRNAVal
-
 ?
6.1.1.9 ATP + DL-2-aminobutyrate + tRNAVal 30% of the activity with valine Escherichia coli AMP + diphosphate + DL-2-aminobutyryl-tRNAVal
-
 ?
6.1.1.9 ATP + DL-allo-2-amino-3-chlorobutyrate + tRNAVal 15% of the activity with valine Escherichia coli AMP + diphosphate + DL-allo-2-amino-3-chlorobutyryl-tRNAVal
-
 ?
6.1.1.9 ATP + DL-threonine + tRNAVal
-
 Escherichia coli AMP + diphosphate + DL-threonyl-tRNAVal
-
 ?
6.1.1.9 ATP + L-threonine + tRNAVal 28% of the activity with valine Escherichia coli AMP + diphosphate + L-threonyl-tRNAVal
-
 ?
6.1.1.9 ATP + L-valine + tRNAVal
-
 Escherichia coli AMP + diphosphate + L-valyl-tRNAVal
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
6.1.1.4 6 9 little difference in activity within this range Escherichia coli
6.1.1.9 8.5 9 in Tris glycine or glycine buffer Escherichia coli

pH Range

EC Number pH Minimum pH Maximum Comment Organism
6.1.1.4 6 9 little difference in activity within this range Escherichia coli