Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Godar, D.E.; Yang, D.C.H.
    Mammalian high molecular weight and monomeric forms of valyl-tRNA synthetase (1988), Biochemistry, 27, 2181-2186.
    View publication on PubMed

General Stability

EC Number General Stability Organism
6.1.1.9 bovine serum albumin protects from thermal inactivation Rattus norvegicus

Inhibitors

EC Number Inhibitors Comment Organism Structure
6.1.1.9 KCl 50% inhibition at 100 mM, optimal activation at 25 mM Rattus norvegicus
6.1.1.9 Mg2+ 50% inhibition at 7 mM, optimal activation at 4 mM Rattus norvegicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.1.1.9 0.0009
-
 tRNAVal tetrameric enzyme form Rattus norvegicus
6.1.1.9 0.00092
-
 tRNAVal monomeric enzyme form Rattus norvegicus
6.1.1.9 0.0082
-
 valine monomeric enzyme form Rattus norvegicus
6.1.1.9 0.01
-
 valine tetrameric enzyme form Rattus norvegicus
6.1.1.9 0.029
-
 ATP tetrameric enzyme form Rattus norvegicus
6.1.1.9 0.071
-
 ATP monomeric enzyme form Rattus norvegicus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
6.1.1.9 KCl optimal concentration: 25 mM Rattus norvegicus
6.1.1.9 KCl 50% inhibition at 100 mM Rattus norvegicus
6.1.1.9 Mg2+ 50% inhibition at 7 mM Rattus norvegicus
6.1.1.9 Mg2+ optimal concentration: 4 mM Rattus norvegicus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
6.1.1.9 140000
-
 1 * 140000, SDS-PAGE Rattus norvegicus
6.1.1.9 140000
-
 4 * 140000, SDS-PAGE, the high molecular weight valyl-tRNA synthetase is a homotypic tetramer which converts to the monomeric valyl-tRNA synthetase after cleavage of a small peptide Rattus norvegicus
6.1.1.9 145000
-
 sucrose density gradient centrifugation, monomeric enzyme form Rattus norvegicus
6.1.1.9 585000
-
 gel filtration, tetrameric enzyme form Rattus norvegicus

Organism

EC Number Organism UniProt Comment Textmining
6.1.1.9 Rattus norvegicus
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
6.1.1.9 liver
-
 Rattus norvegicus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
6.1.1.9 0.722
-
-
 Rattus norvegicus

Storage Stability

EC Number Storage Stability Organism
6.1.1.9 -180°C, purified enzyme highly unstable, stored immediately after purification at -180°C Rattus norvegicus
6.1.1.9 -20°C, homogenate and ammonium sulfate fraction are stable for 6 months Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.1.1.9 ATP + L-valine + tRNAVal
-
 Rattus norvegicus AMP + diphosphate + L-valyl-tRNAVal
-
 ?

Subunits

EC Number Subunits Comment Organism
6.1.1.9 monomer 1 * 140000, SDS-PAGE Rattus norvegicus
6.1.1.9 monomer the high molecular weight valyl-tRNA synthetase is a homotypic tetramer which converts to the monomeric valyl-tRNA synthetase after cleavage of a small peptide Rattus norvegicus
6.1.1.9 tetramer 4 * 140000, SDS-PAGE, the high molecular weight valyl-tRNA synthetase is a homotypic tetramer which converts to the monomeric valyl-tRNA synthetase after cleavage of a small peptide Rattus norvegicus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
6.1.1.9 37
-
-
 Rattus norvegicus

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
6.1.1.9 additional information
-
 no activity detectable at 60°C Rattus norvegicus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
6.1.1.9 4
-
 half-life: 90 min Rattus norvegicus
6.1.1.9 20
-
 half-life: 30 min, when enzyme is stabilized by 1 mg/ml bovine serum albumin and 50% glycerol Rattus norvegicus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
6.1.1.9 6 7.5
-
 Rattus norvegicus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
6.1.1.9 6 8.5 pH 6-7.5: optimum, pH 8.5: 50% of maximal activity Rattus norvegicus

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
6.1.1.9 7
-
 Mg2+
-
 Rattus norvegicus