BRENDA - Enzyme Database show

The metabolism of gamma-hydroxyglutamate in rat liver. 1. Enzymic synthesis of gamma-hydroxy-alpha-ketoglutarate from pyruvate and glyoxylate

Kuratomi, K.; Fukunaga, K.; Biochim. Biophys. Acta 78, 617-628 (1963)

Data extracted from this reference:

Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
4.1.3.16
arsenite
-
Rattus norvegicus
4.1.3.16
Cu2+
93% inhibition at 2 mM
Rattus norvegicus
4.1.3.16
cysteine
65% inhibition at 10 mM
Rattus norvegicus
4.1.3.16
Hg2+
complete inhibition at 2 mM
Rattus norvegicus
4.1.3.16
iodoacetate
10% inhibition at 10 mM
Rattus norvegicus
4.1.3.16
N-ethylmaleimide
64% inhibition at 10 mM
Rattus norvegicus
4.1.3.16
p-chloromercuribenzoate
complete inhibition at 10 mM
Rattus norvegicus
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
4.1.3.16
0.43
-
glyoxylate
-
Rattus norvegicus
4.1.3.16
1.33
-
4-hydroxy-alpha-ketoglutarate
-
Rattus norvegicus
4.1.3.16
10
-
pyruvate
-
Rattus norvegicus
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
4.1.3.16
mitochondrion
-
Rattus norvegicus
5739
-
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
4.1.3.16
additional information
no requirement
Rattus norvegicus
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
4.1.3.16
Rattus norvegicus
-
rat
-
Purification (Commentary)
EC Number
Commentary
Organism
4.1.3.16
-
Rattus norvegicus
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
4.1.3.16
brain
poor activity
Rattus norvegicus
-
4.1.3.16
kidney
-
Rattus norvegicus
-
4.1.3.16
liver
-
Rattus norvegicus
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [Ámol/min/mg]
Specific Activity Maximum [Ámol/min/mg]
Commentary
Organism
4.1.3.16
5.3
-
-
Rattus norvegicus
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4.1.3.16
4-Hydroxy-2-oxoglutarate
-
5237
Rattus norvegicus
Pyruvate + glyoxylate
-
5237
Rattus norvegicus
-
4.1.3.16
Glyoxylate + pyruvate
specific, codensation favoured, Keq: 0.73 mM
5237
Rattus norvegicus
4-Hydroxy-2-ketoglutarate
-
5237
Rattus norvegicus
-
4.1.3.16
additional information
specific for glyoxylate and pyruvate
5237
Rattus norvegicus
?
-
-
-
-
4.1.3.16
additional information
equilibrium favours condensation, Keq: 0.73 1/mM
5237
Rattus norvegicus
?
-
-
-
-
Temperature Optimum [░C]
EC Number
Temperature Optimum [░C]
Temperature Optimum Maximum [░C]
Commentary
Organism
4.1.3.16
37
-
enzyme assay at
Rattus norvegicus
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
4.1.3.16
7.5
8
condensation, elimination
Rattus norvegicus
pH Range
EC Number
pH Minimum
pH Maximum
Commentary
Organism
4.1.3.16
5
9
-
Rattus norvegicus
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
4.1.3.16
arsenite
-
Rattus norvegicus
4.1.3.16
Cu2+
93% inhibition at 2 mM
Rattus norvegicus
4.1.3.16
cysteine
65% inhibition at 10 mM
Rattus norvegicus
4.1.3.16
Hg2+
complete inhibition at 2 mM
Rattus norvegicus
4.1.3.16
iodoacetate
10% inhibition at 10 mM
Rattus norvegicus
4.1.3.16
N-ethylmaleimide
64% inhibition at 10 mM
Rattus norvegicus
4.1.3.16
p-chloromercuribenzoate
complete inhibition at 10 mM
Rattus norvegicus
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
4.1.3.16
0.43
-
glyoxylate
-
Rattus norvegicus
4.1.3.16
1.33
-
4-hydroxy-alpha-ketoglutarate
-
Rattus norvegicus
4.1.3.16
10
-
pyruvate
-
Rattus norvegicus
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
4.1.3.16
mitochondrion
-
Rattus norvegicus
5739
-
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
4.1.3.16
additional information
no requirement
Rattus norvegicus
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
4.1.3.16
-
Rattus norvegicus
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
4.1.3.16
brain
poor activity
Rattus norvegicus
-
4.1.3.16
kidney
-
Rattus norvegicus
-
4.1.3.16
liver
-
Rattus norvegicus
-
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [Ámol/min/mg]
Specific Activity Maximum [Ámol/min/mg]
Commentary
Organism
4.1.3.16
5.3
-
-
Rattus norvegicus
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4.1.3.16
4-Hydroxy-2-oxoglutarate
-
5237
Rattus norvegicus
Pyruvate + glyoxylate
-
5237
Rattus norvegicus
-
4.1.3.16
Glyoxylate + pyruvate
specific, codensation favoured, Keq: 0.73 mM
5237
Rattus norvegicus
4-Hydroxy-2-ketoglutarate
-
5237
Rattus norvegicus
-
4.1.3.16
additional information
specific for glyoxylate and pyruvate
5237
Rattus norvegicus
?
-
-
-
-
4.1.3.16
additional information
equilibrium favours condensation, Keq: 0.73 1/mM
5237
Rattus norvegicus
?
-
-
-
-
Temperature Optimum [░C] (protein specific)
EC Number
Temperature Optimum [░C]
Temperature Optimum Maximum [░C]
Commentary
Organism
4.1.3.16
37
-
enzyme assay at
Rattus norvegicus
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
4.1.3.16
7.5
8
condensation, elimination
Rattus norvegicus
pH Range (protein specific)
EC Number
pH Minimum
pH Maximum
Commentary
Organism
4.1.3.16
5
9
-
Rattus norvegicus