Literature summary extracted from
Dainty, R.H.
Purification and properties of threonine aldolase from Clostridium pasteurianum (1970), Biochem. J., 117, 585-592.
Inhibitors
EC Number |
Inhibitors |
Comment |
Organism |
Structure |
---|
4.1.2.5 |
2-mercaptoethanol |
activity is decreased to 20% of the original by treatment with cysteine plus mercaptoethanol. Most of the loss is regained on incubation with pyridoxal 5'-phosphate |
Clostridium pasteurianum |
|
4.1.2.5 |
cysteine |
activity is decreased to 20% of the original by treatment with cysteine plus mercaptoethanol. Most of the loss is regained on incubation with pyridoxal 5'-phosphate |
Clostridium pasteurianum |
|
4.1.2.5 |
hydroxylamine |
1 mM, complete inhibition |
Clostridium pasteurianum |
|
4.1.2.5 |
Semicarbazide |
1 mM, complete inhibition |
Clostridium pasteurianum |
|
KM Value [mM]
EC Number |
KM Value [mM] |
KM Value Maximum [mM] |
Substrate |
Comment |
Organism |
Structure |
---|
4.1.2.5 |
0.42 |
- |
L-threonine |
pH 7.0, 30°C |
Clostridium pasteurianum |
|
Organism
EC Number |
Organism |
UniProt |
Comment |
Textmining |
---|
4.1.2.5 |
Clostridium pasteurianum |
- |
- |
- |
Purification (Commentary)
EC Number |
Purification (Comment) |
Organism |
---|
4.1.2.5 |
- |
Clostridium pasteurianum |
Storage Stability
EC Number |
Storage Stability |
Organism |
---|
4.1.2.5 |
0-5°C, activity slowly decreases to 50% of the original activity after 10 days |
Clostridium pasteurianum |
Substrates and Products (Substrate)
EC Number |
Substrates |
Comment Substrates |
Organism |
Products |
Comment (Products) |
Rev. |
Reac. |
---|
4.1.2.5 |
L-threonine |
the enzyme is highly specific for L-threonine, no activity with D-threonine, no activity with DL-allo-threonine |
Clostridium pasteurianum |
glycine + acetaldehyde |
- |
r |
|
Synonyms
EC Number |
Synonyms |
Comment |
Organism |
---|
4.1.2.5 |
L-threonine acetaldehyde-lyase |
- |
Clostridium pasteurianum |
pH Optimum
EC Number |
pH Optimum Minimum |
pH Optimum Maximum |
Comment |
Organism |
---|
4.1.2.5 |
6.5 |
7 |
- |
Clostridium pasteurianum |
pH Range
EC Number |
pH Minimum |
pH Maximum |
Comment |
Organism |
---|
4.1.2.5 |
6 |
7.8 |
pH 6.0: about 80% of maximal activity, pH 7.8: about 80% of maximal activity |
Clostridium pasteurianum |
Cofactor
EC Number |
Cofactor |
Comment |
Organism |
Structure |
---|
4.1.2.5 |
pyridoxal 5'-phosphate |
- |
Clostridium pasteurianum |
|